Effect of lyoprotectants on ascorbate oxidase activity after freeze-drying and storage

Process Biochemistry

Volumn 31, Issue 2, 1996, Pages 173-178

  D'Andrea, G ^a   Salucci, M L ^a   Avigliano, L ^b  

^a UNIVERSITY OF L'AQUILA   (Italy)

^b UNIVERSITY OF ROME TOR VERGATA   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0029174702     PISSN: 13595113     EISSN: None     Source Type: Journal    
DOI: 10.1016/0032-9592(95)00045-3     Document Type: Article

References (27)

1. Fee, J. A., In Structure and Bonding, Vol. 23. Springer, Heidelberg, 1975, pp. 2-60.
2. Marchesini, A., Cappelletti, P., Canonica, L., Danieli, B. & Tollari, S., Evidence about the catecholoxidase activity of the enzyme ascorbate oxidase extracted from Cucurbita pepo medullosa. Biochim. Biophys. Acta, 44 (1977) 290-300.
3. Butt, V. S., In The Biochemistry of Plants. A Comprehensive Treatise. Metabolism and Respiration, Vol. 2, ed. A. Dies. Academic Press, London, 1980, pp. 85-95.
4. Lin, L. S. & Varner, J. E., Expression of ascorbic acid oxidase in zucchini squash (Cucurbita pepo L.). Plant Physiol. 96 (1991) 159-65.
5. Messerschmidth, A., Ladenstein, R., Huber, R., Bolognesi, M., Avigliano, L., Petruzzelli, R., Rossi, A. & Finazzi-Agrò, A., Refined crystal structure of ascorbate oxidase at 1.9 Å resolution. J. Mol. Biol., 224 (1992) 170-205.
6. D'Andrea, G., Bouwstra, J. B., Kamerling, J. P. & Vliegenthart, J. F. G., Primary structure of the xylose-containing N-linked carbohydrate moiety from ascorbic acid oxidase of Cucurbita pepo medullosa. Glycoconj. J., 5 (1988) 151-7.
7. Stevanato, R., Avigliano, L., Finazzi-Agrò, A. & Rigo, A., Determination of ascorbic acid with immobilized green zucchini ascorbate oxidase. Anal. Biochem., 149 (1985) 537-42.
8. Greenway, G. M. & Ongomo, P., Determination of L-ascorbic acid in fruit and vegetable juices by flow injection with immobilized ascorbate oxidase. Analyst, 115 (1990) 1297-9.
9. Crawford, G. A., Mahoney, J. F. & Györy, A. Z., Measurement of urinary oxalate in the presence of ascorbic acid. Clin. Chim. Acta, 147 (1985) 51-7.
10. Suzuky, M. & Yoshida, M., A new enzymatic determination of serum creatine. Clin. Chim. Acta, 140 (1984) 289-94.
11. Hanafusa, N., In Freezing and Drying of Microorganisms, ed. T. Nei. University Park Press, Baltimore, 1969, pp. 117-29.
12. Lee, J. C. & Timasheff, S. N., The stabilization of proteins by sucrose. J. Biol. Chem., 256 (1981) 7193-201.
13. Brands, J. F., Fu, J. & Nordin, J. H., In The Frozen Cell, ed. G. E. W. Wolstenholme & M. O'Conner. J. & A. Churchill, London, 1970, pp. 189-212.
14. Nagy, G., Rice, M. E. & Adams, R. N., A new type of enzyme electrode: the ascorbic acid eliminator electrode. Life Sci., 31 (1982) 2611-6.
15. Esaka, M., Suzuki, K. & Kubota, K., Determination method for L-ascorbic acid in foods with immobilized ascorbate oxidase. Agric. Biol. Chem., 49 (1985) 2955-60.
16. Tono, T. & Fujita, S., Spectrophotometric determination based on difference spectra of L-ascorbic acid in plants and animal foods. Agric. Biol. Chem., 46 (1982) 2953-9.
17. Avigliano, L., Vecchini, P., Sirianni, P., Marcozzi, G., Marchesini, A. & Mondovì, B., A reinvestigation on the quaternary structure of ascorbate oxidase from Cucurbita pepo medullosa. Mol. Cell. Biochem., 56 (1983) 107-12.
18. Haselbeck, A. & Hösel, W., Studies on the effect of the incubation conditions, various detergents and protein concentration on the enzymatic activity of N-glycosidase F (glycopeptidase F) and endoglycosidase F. Topics Biochem., 8 (1988) 1-4.
19. Bradford, M. M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72 (1976) 248-54.
20. Hellman, K., Miller, D. S. & Cammack, K. A., The effect of freeze-drying on the quaternary structure of L-asparaginase from Erwinia carotovora. Biochim. Biophys. Acta, 749 (1983) 133-42.
21. Carpenter, J. F., Crowe, L. M. & Crowe, J. H., Stabilization of phosphofructokinase with sugars during freeze-drying: characterization of enhanced protection in the presence of divalent cations. Biochim. Biophys. Acta, 923 (1987) 109-15.
22. Pikal, M. J., Freeze-drying of proteins. Part I: Process design. Pharm. Technol. Int., 3 (1) (1991) 37-43.
23. Pikal, M. J., Freeze-drying of proteins. Part II: Formulation selection. Pharm. Technol. Int., 3 (2) (1991) 40-3.
24. Lis, H. & Sharon, N., Protein glycosylation. Structural and functional aspects. Eur. J. Biol., 218 (1993) 1-27.
25. Schägger, H. & von Jagow, G., Blue native electrophoresis for isolation of membrane protein in enzymatically active form. Anal. Biochem., 199 (1991) 223-31.
26. Schägger, H., Cramer, W. A. & von Jagow, G., Analysis of molecular masses and oligomeric state of protein complexes by blue native electrophoresis and isolation of membrane protein complexes by two-dimensional native electrophoresis. Anal. Biochem., 217 (1994) 220-30.
27. Dabulis, K. & Klibanov, A. M., Dramatic enhancement of enzymatic activity in organic solvents by lyoprotectants. Biotechnol. Bioeng., 41 (1993) 566-71.