Lipofection of insulin-producing RINm5F cells: Methodological improvements

Journal of Liposome Research

Volumn 17, Issue 2, 2007, Pages 49-62

  Barbu, Andreea ^a,b   Welsh, Nils ^a  

^a UPPSALA UNIVERSITY   (Sweden)

^b UPPSALA UNIVERSITY   (Sweden)

Author keywords

Cell death; Gene transfer; Liposomes; NLS; RIN5mF cells

Indexed keywords

1,3 DIOLEOYLOXY 2 (6 CARBOXYSPERMYL)PROPYLAMIDE; ACETYLCYSTEINE; CASPASE 3 INHIBITOR; DENDRIMER; DIOLEOYLPHOSPHATIDYLETHANOLAMINE; DOSPER; FORSKOLIN; FUGENE; FUMONISIN B1; GENEPORTER; GREEN FLUORESCENT PROTEIN; INSULIN; LIPID; LIPOFECTAMINE; LIPOTAXI; N,N[BIS(2 HYDROXYETHYL) N METHYL N [2,3 BIS(TETRADECANOYLOXY)PROPYL]AMMONIUM IODIDE; NICKEL CHLORIDE; NICOTINAMIDE; PHORBOL 13 ACETATE 12 MYRISTATE; POLYAMIDOAMINE; SIGNAL PEPTIDE; SUPERFECT; TRANSFAST; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CELL DEATH; CELLULAR DISTRIBUTION; CENTRIFUGATION; CONTROLLED STUDY; DRUG CYTOTOXICITY; DRUG FORMULATION; FLOW CYTOMETRY; GENE TRANSFER; GENETIC TRANSFECTION; INSULIN SYNTHESIS; LIPOFECTION; LIPOSOMAL GENE DELIVERY SYSTEM; NONHUMAN; NONVIRAL GENE DELIVERY SYSTEM; PRIORITY JOURNAL; PROTEIN LOCALIZATION; RAT; SERUM;

ANIMALS; CARCINOGENS; CELL DEATH; CELL LINE; ENZYME INHIBITORS; FORSKOLIN; FUMONISINS; GENE EXPRESSION; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; INSULIN; LIPIDS; RATS; TETRADECANOYLPHORBOL ACETATE; TRANSFECTION;

EID: 34347378432     PISSN: 08982104     EISSN: 15322394     Source Type: Journal    
DOI: 10.1080/01676830701374986     Document Type: Article

References (49)

1. Abe, A., Miyanohara, A., Friedmann, T. (1998). Polybrene increases the efficiency of gene transfer by lipofection. Gene Ther. 5:708-711.
2. Aronshon, A. I., Hughes, J. A. (1998). Nuclear localisation signal peptides enhance cationic liposome-mediated gene therapy. J. Drug Targeting 5(3):163-169.
3. Barbu, A. R., Akusjärvi, G., Welsh, N. (2002). Adenoviral induced islet cell cytotoxicity is not counteracted by Bcl-2 overexpression. Mol. Med. 8(11):733-741.
4. Barbu, A. R., Akusjärvi, G., Welsh, N. (2005). Adenoviral-mediated transduction of human pancreatic islets: importance of adenoviral genome for cell viability and association with a deficient antiviral response. Endocrinology 146:2406-2414.
5. Barbu, A. R., Bodin, B., Welsh, M., Jansson, L., Welsh, N. (2006). Aperfusion protocol for highly efficient transduction of intact pancreatic islets of Langerhans. Diabetologia 49(10):2388-2391.
6. Bilbao, G., Contreras, J. L., Dmitriev, I., Smyth, C. A., Jenkins, S., Eckhoff, D., Thomas, F., Thomas, J., Curiel D. T. (2002). Genetically modified adenovirus vector containing an RDG peptide in the HI loop of the fiber knob improves gene transfer to nonhuman primate isolated pancreatic islets. Am. J. Transpl. 2:237-243.
7. Branden, L. J., Mohamed, A. J., Smith, C. I. E. (1999) A peptide nucleic acid-nuclear localization signal fusion that meiates nuclear transport of DNA. Nat. Biotechnol. 17:784-787.
8. Brunner, S., Sauer, T., Carotta, S., Cotten, M., Saltik, M., Wagner, E. (2000). Cell cycle dependence of gene transfer by lipoplex polyplex and recombinant adenovirus. Gene Ther. 7:401-407.
9. Chick, W. L., Warren, S., Chute, R. N., Like, A. A., Lauris, V., Kitchen, K. C. (1977). A transplantable insulinoma in the rat. Proc. Natl. Acad. Sci. USA 74(2):628-632.
10. Chuck, A. S., Clarke, M. F., Palsson, B. O. (1996). Retroviral infection is limited by Brownian motion. Hum. Gene. Ther. 7(13):1527-1534.
11. Davis, M. E. (2002). Non-viral gene delivery systems. Curr. Opin. Biotechnol. 13:128-131.
12. Dowty, M. E., Williams, P., Zhang, G., Hagstrom, J. E., Wolff, J. A. (1995). Plasmid DNA entry into postmitotic nuclei of primary rat myotubes. Proc. Natl. Acad. Sci. USA 92:4572-4576.
13. Fuller, M., Anson, D. S. (2004). Can the use of HIV-1 derived gene transfer vectors for clinical application be justified? Curr. Gene Ther. 4(1):65-77.
14. Gainer, A. L., Korbutt, G. S., Rajotte, R. V., Warnock, G. L., Elliot, J. F. (1996). Successful biolistic transformation of mouse pancreatic islets while preserving cellular function. Transplantation 61(11):1567-1571.
15. Godbey, W. T., Mikos, A. G. (2001). Recent progress in gene delivery using non-viral transfer complexes. J. Control. Release 72:115-125.
16. Jans, D. A. (1994). Nuclear signaling pathways for polypeptides ligands and their membrane receptors. FASEB J. 8(11):841-847.
17. Ju, Q., Edelstein, D., Brendel, M. D., Bradhorst, D. (1998). Transduction of non-dividing adult human pancreatic beta cells by an integrating lentiviral vector. Diabetologia 41(6):736-739.
18. Kaneto, H., Kajimoto, Y., Miyagawa et al. (1999). Beneficial effects of antioxidants in diabetes: possible protection of pancreatic beta-cells against glucose toxicity. Diabetes 48(12):2398-2406.
19. Kwon, G., Pappan, K. L., Marshall, C. A., Schaffer, J. E., McDaniel, M. L. (2004). cAMP Dose-dependently prevents palmitate-induced apoptosis by both protein kinase A- and cAMP-guanine exchange factor-dependent pathways in beta-cells. J. Biol. Chem. 279(10):8938-8945.
20. Lechardeur, D., Lukacs, G. L. (2002). Intracellular barriers to non-viral gene transfer. Curr. Gene Ther. 2:183-194.
21. Lendley, F. D. (1997). Nonviral gene therapy: The promise of genes as pharmaceutical products. Hum. Gene Ther. 6(9):1129-1144.
22. Liu, G., Li D., Pasumarthy, M. K., Kowalczyk, T. H., Gedeon, C. R. et al. (2003). Nanoarticles of compacted DNA transfect postmitotic cells. J. Biol. Chem. 278:32578-32586.
23. Maedler, K., Fontana, A., Ris, F., Sergeev, P., Toso, C., Oberholzer, J., Lehmann, R., Bachmann, F., Tasinato, A., Spinas, G. A., Halban, P. A., Donath, M. Y. (2002). FLIP switches Fas-mediated glucose signaling in human pancreatic beta cells from apoptosis to cell replication. Proc. Natl. Acad. Sci. USA 99(12):8236-8241.
24. Maedler, K., Spinas, G. A., Dyntar, D., Moritz, W., Kaiser, N., Donath, M. Y. (2001). Distinct effects of saturated and monosaturated fatty acids on beta-cell turnover and function. Diabetes 50(1):69-76.
25. Mortimer, I., Tam P., MacLachlan, I., Graham, R. W., Saravolac, E. G., Joshi, P. B. (1999). Cationic lipid-mediated transfection of cells in culture requires mitotic activity. Gene Ther. 6:403-411.
26. Navarro-Tableros, V., Sanchez-Soto, M. C., Garcia, S., Hiriart, M. (2004). Autocrine regulation of single pancreatic beta-cell survival. Diabetes 53(8):2018-2023.
27. Nishikawa, M., Huang, L. (2001). Nonviral vectors in the new millennium: delivery barriers in gene transfer. Hum. Gene Ther. 12:861-870.
28. Piro, S., Anello, M., Di Poetro, C. et al. (2002). Chronic exposure to free fatty acids or high glucose induces apoptosis in rat pancreatic islets: possible role of oxidative stress. Metabolism 51(10):1340-1347.
29. Pollard, H., Remy, J. S., Loussouarn, G., Demolombe, S., Behr, J. P., Escande, D. (1998). Polyethilenimine but not cationic lipids promotes transgene delivery to the nucleus in mammalian cells. J. Biol. Chem. 273:7507-7511.
30. Prasad, K. M., Yang, Z., Bleich, D., Nadler, J. L. (2000). Adeno-associated virus vector mediated gene transfer to pancreatic beta cells. Gene Ther. 7(18):1553-1561.
31. Rabinovitch, A., Suarez-Pinzon, W., Strynadka, K., Ju, Q., Edelstein, D., Brownlee, M., Korbutt, G. S., Rajotte, R. V. (1999). Transfection of human pancreatic islets with an anti-apoptotic gene (bcl-2) protects beta-cells from cytokine-induced destruction. Diabetes 48(6):1223-1229.
32. Roth, C. M., Sundaram, S. (2004). Engineering synthetic vectors for improved DNA dlivery: Insights from intracellular pathways. Annu. Rev. Biomed. Eng. 6:397-426.
33. Schwartz, B., Ivanov, M. A., Pitard, B., Escriou, V., Rangara, R., Byk G., Wils, P., Crouzet, J., Sherman, D. Synthetic DNA-compacting peptides derived from human sequence enhance cationic lipid-mediated gene transfer in vitro and in vivo. Gene Ther. 6:282-292.
34. Sebestyen, M. G. et al. (1998). DNA vector chemistry: the covalent attachment of signal peptides to plasmid DNA. Nat. Biotechnol. 16:80-85.
35. Subramanian, A., Ranganathan, P., Diamond, S. L. (1999). Nuclear targeting peptide scafolds for lipofection of nondividing mamalian cells. Nat. Biotechnol. 17:873-877.
36. Trivedi, R. A., Dickson, G. (1995). Liposome-mediated gene transfer into normal and dystrophin-deficient mouse myoblasts. J. Neurochem. 64(5):2230-2238.
37. Uchida, E., Mizuguchi, H., Ishii-Watabe, A., Hayakawa, T. (2002). Comparison of the efficiency and safety of non-viral vector-mdiated gene transfer into a wide range of human cells. Biol. Pharm. Bull. 25(7):891-897.
38. Varga, C. M., Wickham, T. J., Lauffenburger, D. A. (2000). Recptor-mediated targeting of gene delivery vectors: insights from molecular mechanisms for improved vehicle design. Biotechnol. Bioeng. 70:593-605.
39. Verma, R. S., Giannola, D., Shlomchik, W., Emerson, S. G. (1998). Increased efficiency of liposome-mediated transfection by volume reduction and centrifugation. BioTechniques 25(1):46-49.
40. Wang, L., Bhattacharjee, A., Zuo, Z. et al. (1999). A low voltage-activated Ca2+ current mediates cytokine-induced pancreatic beta-cell death. Endocrinology 140(3):1200-1204.
41. Welsh, N., Öberg, C., Hellerström, C., Welsh, M. (1990). Liposome mediated in vitro transfection of pancreatic islet cells. Biomed. Biophys. Acta 49(12):1255-1262.
42. Wiethoff, C. M., Gill, M. L., Koe, G. S., Koe J. G., Middaugh C. R. (2002). The structural organization of cationic lipid-DNA complexes. J. Biol. Chem. 277:44980-44987.
43. Yang, J., Huang, L. Overcomming the inhibitory effect of serum on lipofection by increasing the charge ratio of cationic liposome to DNA. Gene Ther. 4:891-900.
44. Zanta, M. A., Belguise-Valladier, P., Behr J. P. (1999). Gene delivery: A single localisation signal peptide is sufficient to carry DNA to the cell nucleus. Proc. Natl. Acad. Sci. USA 96(1):91-96.
45. Zelphati, O., Szoka, F. C. (1996). Mechanism of oligonucleotide release from cationic liposomes. Proc. Natl. Acad. Sci. USA 93(21):11493-11498.
46. Zhang, S., Liu, J., Dragunow, M., Cooper, G. J. (2003). Fibrillogenic amylin evokes islet ceta-cell apoptosis through linked activation of a caspase cascade and JNK1. J. Biol. Chem. 278(52):52810-52819.
47. Zhdanov, R. I., Podobed, O. V., Vlassov, V. V. (2002). Cationic lipid-DNA complexes-lipoplexes for gene transfer and therapy. Biochemistry 58:53-64.
48. Zhuang, S., Lynch, M. C., Kochevar, I. E. (1998). Activation of protein kinase C is required for protection of cells against apoptotsis induced by singlet oxygen. FEBS Lett. 437(1-2):158-162.
49. Zuhorn, I. S., Kalicharan, R., Hoekstra, D. (2002). Lipoplex-mediated transfection of mammalian cells occurs through the cholesterol-dependent clathrin-mediated pathway of endocytosis. J. Biol. Chem. 277(20):18021-18028.