Structure and conformational changes in the C-terminal domain of the β2-adrenoceptor: Insights from fluorescence resonance energy transfer studies

Journal of Biological Chemistry

Volumn 282, Issue 18, 2007, Pages 13895-13905

  Granier, Sébastien ^a   Kim, Samuel ^a   Shafer, Aaron M ^a   Ratnala, Venkata R P ^a   Fung, Juan José ^a   Zare, Richard N ^a   Kobilka, Brian ^a,b  

^a STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b STANFORD UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIVATION ENERGY; CELL CULTURE; CONFORMATIONS; ENERGY TRANSFER; FLUORESCENCE; MOLECULAR STRUCTURE;

CYTOPLASMIC END; FLUORESCENCE RESONANCE ENERGY TRANSFER (FRET); TRANSMEMBRANES;

PROTEINS;

3 ISOPROPYLAMINO 1 (7 METHYL 4 INDANYLOXY) 2 BUTANOL; ALPHA 2 ADRENERGIC RECEPTOR; ALPHA 2 ADRENERGIC RECEPTOR STIMULATING AGENT; BETA 2 ADRENERGIC RECEPTOR; BETA ADRENERGIC RECEPTOR BLOCKING AGENT; CYSTEINE; LIGAND; PROPANOLAMINE DERIVATIVE; RECOMBINANT PROTEIN; RETINA S ANTIGEN;

ARTICLE; CARBOXY TERMINAL SEQUENCE; CONFORMATIONAL TRANSITION; FLUORESCENCE RESONANCE ENERGY TRANSFER; LIGAND BINDING; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN MOTIF; PROTEIN STRUCTURE; RECEPTOR BINDING; REGULATORY MECHANISM; SIGNAL TRANSDUCTION; STRUCTURE ANALYSIS; ANIMAL; CHEMISTRY; GENETICS; HUMAN; METABOLISM; PHYSIOLOGY; PROTEIN BINDING; PROTEIN TERTIARY STRUCTURE; STRUCTURE ACTIVITY RELATION;

ALEXA;

ADRENERGIC BETA-ANTAGONISTS; AMINO ACID MOTIFS; ANIMALS; ARRESTIN; CYSTEINE; FLUORESCENCE RESONANCE ENERGY TRANSFER; HUMANS; LIGANDS; PROPANOLAMINES; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; RECEPTORS, ADRENERGIC, BETA-2; RECOMBINANT PROTEINS; SIGNAL TRANSDUCTION; STRUCTURE-ACTIVITY RELATIONSHIP;

EID: 34250376066     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M611904200     Document Type: Article

References (31)

1. Ji, T. H., Grossmann, M., and Ji, I. (1998) J. Biol. Chem. 273, 17299-17302
2. Ballesteros, J. A., Shi, L., and Javitch, J. A. (2001) Mol. Pharmacol. 60, 1-19
3. Farrens, D. L., Altenbach, C., Yang, K., Hubbell, W. L., and Khorana, H. G. (1996) Science 274, 768-770
4. Altenbach, C., Cai, K., Khorana, H. G., and Hubbell, W. L. (1999) Biochemistry 38, 7931-7937
5. Cai, K., Klein-Seetharaman, J., Farrens, D., Zhang, C., Altenbach, C., Hubbell, W. L., and Khorana, H. G. (1999) Biochemistry 38, 7925-7930
6. Altenbach, C., Klein-Seetharaman, J., Cai, K., Khorana, H. G., and Hubbell, W. L. (2001) Biochemistry 40, 15493-15500
7. Swaminath, G., Xiang, Y., Lee, T. W., Steenhuis, J., Parnot, C., and Kobilka, B. K. (2004) J. Biol. Chem. 279, 686-691
8. Ghanouni, P., Gryczynski, Z., Steenhuis, J. J., Lee, T. W., Farrens, D. L., Lakowicz, J. R., and Kobilka, B. K. (2001) J. Biol. Chem. 276, 24433-24436
9. Ghanouni, P., Steenhuis, J. J., Farrens, D. L., and Kobilka, B. K. (2001) Proc. Natl. Acad. Sci. U. S. A. 98, 5997-6002
10. Benovic, J. L. (2002) J. Allergy Clin. Immunol. 110, Suppl. 6, S229-S235
11. Reiter, E., and Lefkowitz, R. J. (2006) Trends Endocrinol. Metab. 17, 159-165
12. Lefkowitz, R. J., and Shenoy, S. K. (2005) Science 308, 512-517
13. Ren, X. R., Reiter, E., Ahn, S., Kim, J., Chen, W., and Lefkowitz, R. J. (2005) Proc. Natl. Acad. Sci. U. S. A. 102, 1448-1453
14. Azzi, M., Charest, P. G., Angers, S., Rousseau, G., Kohout, T., Bouvier, M., and Pineyro, G. (2003) Proc. Natl. Acad. Sci. U. S. A. 100, 11406-11411
15. Okada, T., Sugihara, M., Bondar, A. N., Elstner, M., Entel, P., and Buss, V. (2004) J. Mol. Biol. 342, 571-583
16. Gether, U., Lin, S., Ghanouni, P., Ballesteros, J. A., Weinstein, H., and Kobilka, B. K. (1997) EMBO J. 16, 6737-6747
17. Swaminath, G., Deupi, X., Lee, T. W., Zhu, W., Thian, F. S., Kobilka, T. S., and Kobilka, B. (2005) J. Biol. Chem. 280, 22165-22171
18. Ha, T., Ting, A. Y., Liang, J., Caldwell, W. B., Deniz, A. A., Chemla, D. S., Schultz, P. G., and Weiss, S. (1999) Proc. Natl. Acad. Sci. U. S. A. 96, 893-898
19. Chen, R. F. (1965) Science 150, 1593-1595
20. Magde, D., Wong, R., and Seybold, P. G. (2002) Photochem. Photobiol. 75, 327-334
21. O'Dowd, B. F., Hnatowich, M., Caron, M. G., Lefkowitz, R. J., and Bouvier, M. (1989) J. Biol. Chem. 264, 7564-7569
22. Dohlman, H. G., Caron, M. G., DeBlasi, A., Frielle, T., and Lefkowitz, R. J. (1990) Biochemistry 29, 2335-2342
23. Griffin, B. A., Adams, S. R., and Tsien, R. Y. (1998) Science 281, 269-272
24. Griffin, B. A., Adams, S. R., Jones, J., and Tsien, R. Y. (2000) Methods Enzymol. 327, 565-578
25. Adams, S. R., Campbell, R. E., Gross, L. A., Martin, B. R., Walkup, G. K., Yao, Y., Llopis, J., and Tsien, R. Y. (2002) J. Am. Chem. Soc. 124, 6063-6076
26. Trester-Zedlitz, M., Burlingame, A., Kobilka, B., and von Zastrow, M. (2005) Biochemistry 44, 6133-6143
27. Yao, X., Parnot, C., Deupi, X., Ratnala, V. R., Swaminath, G., Farrens, D., and Kobilka, B. (2006) Nat. Chem. Biol. 2, 417-422
28. Lakowicz, J. R. (2006) Principles of Fluorescence Spectroscopy, 3rd Ed., Springer Science+Business Media, LLC, New York
29. Dyson, H. J., and Wright, P. E. (2005) Nat. Rev. Mol. Cell. Biol. 6, 197-208
30. Tompa, P., Szasz, C., and Buday, L. (2005) Trends Biochem. Sci. 30, 484-489
31. Hoffmann, C., Gaietta, G., Bunemann, M., Adams, S. R., Oberdorff-Maass, S., Behr, B., Vilardaga, J. P., Tsien, R. Y., Ellisman, M. H., and Lohse, M. J. (2005) Nat. Methods 2, 171-176