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o reported for spontaneous dissociation of ligand/receptor bonds range from a fraction of a second to 100 s or more.
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o reported for spontaneous dissociation of ligand/receptor bonds range from a fraction of a second to 100 s or more.
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-10 g wt).
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β), suggesting that the energy landscape changes to increase the length gained in the direction of force when the bond breaks.
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β), suggesting that the energy landscape changes to increase the length gained in the direction of force when the bond breaks.
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Advanced computational methods like the steered molecular dynamics described in the companion review by Sotomayor and Schulten (36) provide valuable tools for investigating how variations in chemical structure affect activation energy barriers and pathways governing bond strength.
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Advanced computational methods like the "steered molecular dynamics" described in the companion review by Sotomayor and Schulten (36) provide valuable tools for investigating how variations in chemical structure affect activation energy barriers and pathways governing bond strength.
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f (pN/nm) to report the force history f(t). Bond events are identified by the cycles showing periods of probe stretch ending in precipitous recoil, as sketched in Fig. 2A.
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f (pN/nm) to report the force history f(t). Bond events are identified by the cycles showing periods of probe stretch ending in precipitous recoil, as sketched in Fig. 2A.
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As lipid material flows onto a tether, bilayer-spanning proteins (especially those that interact with the cytoskeleton) are expected to remain behind in the cell membrane. However, the acylated proteins bound weakly to the bilayer could build up at the base of the tether, causing some to be expelled from the surface when approaching the tether-cell junction
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Supported by grants from the National Institutes of Health
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Supported by grants from the National Institutes of Health.
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