Untangling desmosomal knots with electron tomography

Science

Volumn 302, Issue 5642, 2003, Pages 109-113

  He, Wanzhong ^a   Cowin, Pamela ^a   Stokes, David L ^a,b  

^a NEW YORK UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b NEW YORK STRUCTURAL BIOLOGY CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADHESION; CRYSTAL STRUCTURE; INTERFACES (MATERIALS); MOLECULES; TOMOGRAPHY;

DESMOSOMAL KNOTS;

CELLS;

C CADHERIN; CADHERIN; TRYPTOPHAN; UNCLASSIFIED DRUG;

ELECTRON; TOMOGRAPHY;

AMINO ACID SUBSTITUTION; ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CELL ADHESION; CELL INTERACTION; CELL JUNCTION; CRYSTAL STRUCTURE; DESMOSOME; ELECTRON THERAPY; MOUSE; NEWBORN; NONHUMAN; PRIORITY JOURNAL; PROTEIN INTERACTION; SKIN; THREE DIMENSIONAL IMAGING; X RAY;

ANIMALS; ANIMALS, NEWBORN; CADHERINS; CELL ADHESION; CRYSTALLOGRAPHY, X-RAY; CYTOSKELETAL PROTEINS; DESMOPLAKINS; DESMOSOMES; DIMERIZATION; EPIDERMIS; FREEZE SUBSTITUTION; HYDROPHOBICITY; IMAGE PROCESSING, COMPUTER-ASSISTED; MICE; MICROSCOPY, ELECTRON; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN STRUCTURE, TERTIARY; TOMOGRAPHY; TRYPTOPHAN; XENOPUS PROTEINS;

ANIMALIA;

EID: 0141865704     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1086957     Document Type: Article

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48. We thank Y. Ji, M. Auer, and K. MacDonald for help in preparing samples; F. Macaluso and J. Ault for assistance with high-pressure freezing; and K. Tayl r, E. Branlund, N. Kisseberth, and D. Mastronarde for providing facilities and support for tomographic data collection and image reconstruction. Coordinates for Fig. 2, G to I, have been deposited in the Protein Data Bank with accession codes 1Q55, 1Q5A, 1Q5B, and 1Q5C. Tomographic reconstructions have been submitted to the electron microscopy database of the European Bioinformatics Institute with accession codes EMD-1051, EMD-1052, and EMD-1053. Supported by NIH grant R01 GM47429 (P.C.).