BJ-48, a novel thrombin-like enzyme from the Bothrops jararacussu venom with high selectivity for Arg over Lys in P1: Role of N-glycosylation in thermostability and active site accessibility

Toxicon

Volumn 50, Issue 1, 2007, Pages 18-31

  Silva Junior, Floriano P ^a   Guedes, Herbert L M ^a   Garvey, Laura C ^c   Aguiar, Aniesse S ^d   Bourguignon, Saulo C ^b   Di Cera, Enrico ^c   Giovanni De Simone, Salvatore ^a,b  

^a INSTITUTO OSWALDO CRUZ   (Brazil)

^b FLUMINENSE FEDERAL UNIVERSITY   (Brazil)

^c WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

^d Instituto Vital Brazil   (Brazil)

Author keywords

Carbohydrate; Clotting; Serine protease; Snake venom; Toxin

Indexed keywords

4 AMINOBENZAMIDINE; AGAROSE; AMINOCAPROIC ACID; ANTITHROMBIN; APROTININ; ARGININE; BATROXOBIN; CARBOHYDRATE DERIVATIVE; DITHIOTHREITOL; ESTERASE; FIBRINOGEN; FIBRINOPEPTIDE A; HIRUDIN; KETONE DERIVATIVE; LYSINE; N [N (3 CARBOXYOXIRANE 2 CARBONYL)LEUCYL]AGMATINE; PROTEIN C; PROTEINASE ACTIVATED RECEPTOR 1; SERINE PROTEASE BJ 48; SERINE PROTEINASE; SIALIC ACID DERIVATIVE; SIALIDASE; SOYBEAN TRYPSIN INHIBITOR; THROMBIN; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; ALPHA CHAIN; ARTICLE; BETA CHAIN; CATALYSIS; ELECTROSPRAY MASS SPECTROMETRY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; GEL FILTRATION CHROMATOGRAPHY; GLYCOSYLATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYDROLYSIS; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN FUNCTION; PURIFICATION; THERMOSTABILITY;

ANIMALS; ARGININE; BOTHROPS; CATALYTIC DOMAIN; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, GEL; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CROTALID VENOMS; ENZYME STABILITY; FIBRINOGEN; GLYCOSYLATION; HYDROGEN-ION CONCENTRATION; LYSINE; SERINE ENDOPEPTIDASES; SERINE PROTEINASE INHIBITORS; SUBSTRATE SPECIFICITY; TEMPERATURE; THROMBIN;

BOTHROPS JARARACUSSU; GLYCINE MAX;

EID: 34249744642     PISSN: 00410101     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.toxicon.2007.02.018     Document Type: Article

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