Purification and partial characterization of a thrombin-like/gyroxin enzyme from bushmaster (Lachesis muta rhombeata) venom

Toxicon

Volumn 34, Issue 5, 1996, Pages 555-565

  Aguiar, A S ^a   Alves, C R ^b   Melgarejo, A ^a   Giovanni De Simone, S ^b,c  

^a Instituto Vital Brazil   (Brazil)

^b INSTITUTO OSWALDO CRUZ   (Brazil)

^c FLUMINENSE FEDERAL UNIVERSITY   (Brazil)

Author keywords

[No Author keywords available]

Indexed keywords

ENZYME; GLYCOPROTEIN; SNAKE VENOM;

ARTICLE; ENZYME ANALYSIS; GEL FILTRATION CHROMATOGRAPHY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PURIFICATION; TOXIN ANALYSIS;

EID: 0029945766     PISSN: 00410101     EISSN: None     Source Type: Journal    
DOI: 10.1016/0041-0101(95)00159-X     Document Type: Article

References (41)

1. Baramova, E. N., Shannon, J. D., Bjarnason, J. B. S. L. and Fox, J. W. (1990) Interaction of hemorrhagic metalloproteinases with human α2-macroglobulin. Biochemistry 29, 1069-1074.
2. Barret, A. J. and Starkey, P. M. (1973) The interaction of α2-macroglobulin with proteinases: characteristics and specificity of the reaction, and a hypothesis concerning its molecular mechanism. Biochem. J. 133, 709-724.
3. Earlanger, B. F., Kokowsky, N. and Cohen, W. (1961) The preparation and properties of two new chromogenic substrates of trypsin. Archs Biochem. Biophys. 95, 271-278.
4. Esnouf, M. P. and Tunnah, G. W. (1967) The isolation and properties of the thrombin-like principle from Agkistrodon rhodostoma venom. Br. J. Haemat. 13, 581-590.
5. Haad, J.S. (1980) Accidentes humans por las sierpentes de los generos Bothrops y Lachesis. Mem. Inst. Butantan 44/45, 403-423.
6. Holleman, W. H. and Coen, L. J. (1970) Characterization of peptides released from human fibrinogen by arvin. Biochim. biophys. Acta 200, 587-589.
7. Holleman, W. H. and Weiss, L. J. (1976) The thrombin-like enzyme from Bothrops atrox snake venom. Properties of the enzyme purified by affinity chromatography on p-aminobenzamidine substituted agarose. J. biol. Chem. 251, 1663-1669.
8. Itoh, N., Tamaka, N., Mihashi, S. and Yamashina, I. (1987) Molecular cloning and sequence analysis of cDNAS for batroxobin, a thrombin-like snake venom enzyme. J. biol. Chem. 262, 3132-3135.
9. Komori, Y., Nakai, T. and Sugihara, H. (1988) Biochemical and physiological studies on a kallikrein-like enzyme from the venom of Crotalus viridis viridis (Prairie rattlesnake). Biochim. biophys. Acta 967, 92-102.
10. Kress, L. F. and Catanesse, J. J. (1981) Effects of human plasma α2-macroglobulin on the proteolytic activity of snake venoms. Toxicon 19, 501-507.
11. Laemmli, U. K. (1970) Cleavage of structural protein during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
12. Lomonte, B. (1985) Edema-forming activity of bushmaster (Lachesis muta stenophrys) and Central America rattlesnake (Crotalus durissus durissus) venoms and neutralization by a polyvalent antivenom. Toxicon 23, 173-176.
13. Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Randall, R. J. (1951) Protein measurement with Folin phenol reagent. J. biol. Chem. 193, 265-275.
14. Magalhães, A., De Oliveira, G. J. and Diniz, C. R. (1981) Purification and partial characterization of a thrombin-like enzyme from the venom of the Bushmaster snake, Lachesis muta noctivaga. Toxicon 19, 279-294.
15. Magalhães, A., Fonseca, B. C. B., Diniz, C. R., Gilroy, J. and Richardson, M. (1993) The complete amino acid sequence of a thrombin-like enzyme/gyroxin analogue from venom of the bushmaster snake (Lachesis muta muta). FEBS 329, 116-120.
16. Markland, F. S. (1976) Crotalase. In: Methods in Enzymology, vol. 45, pp. 223-236 (Lorand, L., Ed.). New York: Academic Press.
17. Markland, F. S. and Damus, P. S. (1971) Purification and properties of a thrombin-like enzyme from the venom of Crotalus adamanteus (Eastern Diammondback Rathesnake). J. biol. Chem. 246, 6460-6473.
18. Ménard, R. and Storer, A. C. (1992) Oxyanion hole interaction in serine and cysteine proteinases. Biol. Chem. Hoppe Seyler 373, 393-400.
19. Niellsen, B. L. and Brow, L. R. (1984) The basis for colored silver protein complex formation in stained polyacrylamide gel. Analyt. Biochem. 141, 311-315.
20. Nokihara, K., Morita, N., Yamaguchi, M. and Watanable, T. (1992) Sequence analysis of cysteine and cystine containing peptides and proteins by a gas-phase sequencer with isocratic separation of PTH-amino acids. Anal. Lett. 25, 513-533.
21. Nolan, C., Hall, L. S. and Barlow, G. H. (1976) Ancrod, the coagulating enzyme from Malayan pit viper (Agkistrodon rhodostoma) venom. In: Methods in Enzymology, vol. 45, pp. 205-213 (Lorand, L., Ed.). New York: Academic Press.
22. Ouchterlony, O. and Nilson, L. A. (1973) In: Handbook of Experimental Immunology, 2nd Edn, Chap. 19 (Weiss, D. M., Ed.). Oxford: Blackwell Scientific.
23. Ouyang, C., Hong, J. S. and Teng, C. M. (1971) Purification and properties of the thrombin-like principle of Agkistrodon acutus venom and its comparison with bovine thrombin. Thromb. Diath. Haemorrh. 26, 224-234.
24. Ouyang, C. and Yang, F. Y. (1974) Purification and properties of the thrombin-like enzyme from Trimeresurus gramineus venom. Biochim. biophys. Acta 351, 354-363.
25. Pirkle, H., Markland, F. S., Theodor, I., Baumgartener, R., Bajwa, S. S. and Kirakossian, H. (1981) The primary structure of crotalase, a thrombin-like venom enzyme, exhibts closer homology to kallikrein than to other serine proteases. Biochem. biophys. Res. Commun. 99, 715-721.
26. Pirkle, H., Theodor, I., Miyada, D. and Simons, G. (1986) Thrombin-like enzyme from the venom of Bitis gabonica. Purification, properties, and coagulant actions. J. biol. Chem. 261, 8830-8835.
27. Polgár, L. and Halás, P. (1982) Current problems in mechanistic studies of serine and cysteine proteinases. Biochem. J. 207, 1-10.
28. Rosing, J. and Tass, G. (1992) Structural and functional properties of snake venoms prothrombin activators. Toxicon 30, 1515-1527.
29. Sanchez, E. F., Magalhaes, A. and Diniz, C. R. (1987) Purification of a hemorrhagic factor (LHF-1) from the venom of the bushmaster snake, Lachesis muta muta. Toxicon 25, 611-619.
30. Selistre, H. S. and Giglio, J. R. (1987) Isolation and characterization of a thrombin-like enzyme from the venom of the snake Bothrops insularis (jararaca ilhoa). Toxicon 25, 1134-1144.
31. Shieh, T. C., Kawabata, S., Kihara, H., Ohno, M. and Iwanaga, S. (1988) Amino acid sequence of a coagulant enzyme, Flavoxobin, from Trimeresurus flavoviridis venom. J. Biochem. 103, 596-605.
32. Silva, N. J., Aird, S. D., Seebart, C. and Kaiser, I.I. (1989) A gyroxin analog from the venom of the Bushmaster (Lachesis muta muta). Toxicon 27, 763-771.
33. Silveira, A. M. V., Magalhaes, A., Diniz, C. R. and De Oliveira, E. B. (1989) Purification and properties of the thrombin-like enzyme from the venom of Lachesis muta muta. Int. J. Biochem. 21, 863-871.
34. Stocker, K., Fischer, H. and Meier, J. (1982) Thrombin-like snake venom proteinases. Toxicon 20, 265-273.
35. Tamanour, Y., Takahashi, T. and Takahashi, K. (1993) Purification and characterization of two isoforms of serine proteinase from the microsomal membrane of rat liver. J. Biochem. 113, 229-235.
36. Teng, C. M., Wang, J. P., Huang, T. F. and Liau, M. Y. (1989) Effects of venom proteases on peptide chromogenic substrates and bovine prothrombin. Toxicon 27, 161-167.
37. Tschesche, H., Mair, G., Godec, G., Fiedler, F., Ehret, W., Hirschaver, C., Lemon, M. and Fritz, H. (1979) Ad. Exp. Med. Biol. (Knins II: Biochemistry Pathophysiology and Clinical Aspects) 120 (part A), 245.
38. Yarleque, A., Campos, S., Escobar, E., Lazo, F., Sanchez, N., Hyslop, S., Marsh, N. A., Butterworth, P. J. and Price, R. G. (1989) Isolation and characterization of a fibrinogen-clotting enzyme from venom of the snake Lachesis muta muta (Peruvian Bushmaster). Toxicon 27, 1189-1197.
39. Warren, L. (1959) The thiobarbituric acid assay of sialic acids. J. biol. Chem. 234, 1971-1975.
40. Werb, Z., Burleigh, M. C., Barret, A. J. and Starkey, P. M. (1974) The interaction of α2-macroglobulin with proteinases: binding and inhibition of mammalian collagenases and other metal proteinases. Biochem. J. 139, 359-368.
41. Winzler, R. J. (1961) Determination of serum glycoprotein. In: Methods of Biochemical Analysis, pp. 290-292 (Glick, D., Ed.). New York: Interscience.