A novel fibrinogen-clotting enzyme, TL-BJ, from the venom of the snake Bothrops jararaca: Purification and characterization

Thrombosis and Haemostasis

Volumn 83, Issue 3, 2000, Pages 438-444

  Serrano, Solange M T ^a   Sampaio, Claudio A M ^b   Mentele, Reinhard ^c   Camargo, Antonio C M ^a   Fink, Edwin ^c  

^a INSTITUTO BUTANTAN   (Brazil)

^b FEDERAL UNIVERSITY OF SÃO PAULO   (Brazil)

^c UNIVERSITY OF MUNICH   (Germany)

Author keywords

Fibrinogen clotting; Purification; Serine peptidase; Snake venom

Indexed keywords

AMMONIUM SULFATE; BENZAMIDINE DERIVATIVE; BENZYLSULFONYL FLUORIDE; FIBRINOGEN; FIBRINOPEPTIDE A; HIRUDIN; SERINE PROTEINASE; VENOM;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; BLOOD CLOTTING; CHROMATOGRAPHY; COMPETITIVE INHIBITION; ENZYME INHIBITION; ENZYME PURIFICATION; MOLECULAR WEIGHT; NONHUMAN; PRIORITY JOURNAL; SNAKE;

EID: 0034098978     PISSN: 03406245     EISSN: None     Source Type: Journal    
DOI: 10.1055/s-0037-1613834     Document Type: Article

References (41)

1. Macfarlane RG, Russell's viper venom. Br J Haematol 1967; 13: 437-51.
2. Stocker KF. In: Medical use of snake venom proteins, Stocker KF (ed.). Boca Raton: CRC Press 1990: 97-160.
3. Hutton RA, Warrel DA. Action of venom components on the haemostatic system. Blood Rev 1993; 7: 176-89.
4. Pirkle H. Thrombin-like enzymes from snake venoms: an updated inventory. Thromb Haemost 1998; 79:675-83.
5. von Klobusitzky D, König P. Estudos biochimicos sobre os venenos das serpentes do gênero Bothrops. Mem. Inst. Butantan (São Paulo) 1935/36; 10: 223-36.
6. Denson KWE, Rousseau WE. Separation of the coagulant components of Bothrops jararaca venom. Toxicon 1970; 8:15-9.
7. Furukawa Y, Hayashi K. Factor X converting and thrombin-like activities of Bothrops jararaca snake venom. Toxicon 1977; 15: 107-14.
8. Nahas L, Kamiguti AS, Barros MAR. Thrombin-like and factor X-activator components of Bothrops snake venoms. Thromb Haemost 1979; 41: 314-28.
9. Nishida S, Fujimura Y, Miura S, Ozaki Y, Usami Y, Suzuki M, Titani K, Yoshida E, Sugimoto M, Yoshioka A, Fukui H. Purification and characterization of bothrombin, a fibrinogen-clotting serine protease from the venom of Bothrops jararaca. Biochemistry 1994; 33: 1843-9.
10. Serrano SMT, Mentele R, Sampaio CAM, Fink E. Purification, characterization, and amino acid sequence of a serine endopeptidase, PA-BJ, with platelet-aggregating activity from the venom of Bothrops jararaca. Biochemistry 1995; 34: 7186-93.
11. Serrano SMT, Hagiwara Y, Murayama N, Higuchi S, Mentele R, Sampaio CAM, Camargo ACM, Fink E. Purification and characterization of a kinin-releasing and fibrinogen-clotting serine endopeptidase (KN-BJ) from the venom of Bothrops jararaca, and molecular cloning and sequence analysis of its cDNA. Eur J Biochem 1998; 251: 845-53.
12. Henriques OB, Fichman M, Henriques SB. Partial purification and some properties of the blood-clotting factor from the venom of Bothrops jararaca. Biochem J 1960; 75: 551-6.
13. Fink E, Schill W-B, Fiedler F, Krasnigg G, Geiger R, Shimamoto K. Tissue kallikrein of human seminal plasma is secreted by the prostate gland. Biol Chem Hoppe-Seyler 1985; 366: 917-24.
14. Mustard JF, Perry DW, Ardlie NG, Packham MA. Preparation of suspensions of washed platelets from humans. Br J Haematol 1972; 22: 193-204.
15. Fiedler F, Geiger R, Hirschauer C, Leysath G. Peptide esters and nitroanilides as substrates for the assay of human urinary kallikrein. Hoppe-Seyler's Z Physiol Chem 1978; 259: 1667-73.
16. Jameson GW, Roberts DV, Adams RW, Kyle WSA, Elmore DT. Determination of the operational molarity of solutions of bovine α-chymotrypsin, trypsin, thrombin and factor Xa by spectrofluorimetric titration. Biochem J 1973; 131: 107-17.
17. Stürzebecher J, Stürzebecher U, Markwardt F. Inhibition of batroxobin, a serine endopeptidase from Bothrops snake venom, by derivatives of benzamidine. Toxicon 1986; 24: 585-95.
18. Dixon M. The determination of enzyme inhibitor constants. Biochem J 1953; 55: 170-1.
19. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteoriophage T4. Nature 1970; 227: 680-5.
20. Heukeshoven J, Dernick R. Simplified method for silver staining of proteins in polyacrylamide gels and the mechanism of silver staining. Electrophoresis 1985; 6: 103-12.
21. Friedman M, Krull LH, Cavins JF. The Chromatographic determination of cystine and cysteine residues in proteins as S-beta-(4-pyridylethyl)cysteine. J Biol Chem 1970; 245: 3868-71.
22. Lis H, Sharon N. Protein glycosylation. Structural and functional aspects. Eur J Biochem 1993; 218:1-27.
23. Pirkle H, Thrombin-like enzymes from snake venoms: an updated inventory. Thromb Haemost 1998; 79: 675-83.
24. Tanaka N, Nakada H, Itoh N, Mizuno Y, Takanishi M, Kawasaki T, Tate S, Inagaki F, Yamashina I. Novel structure of the N-acetylgalactosamine containing N-glycosidic carbohydrate chain of batroxobin, a thrombin-like snake venom enzyme. J. Biochem. 1992; 112: 68-74.
25. Blombäck B, Blombäck M, Hessel B, Iwanaga S. Structure of N-terminal fragments of fibrinogen and specificity of thrombin. Nature 1967; 215: 1445-8.
26. Nolan C, Hall LS, Barlow GH. Ancrod, the coagulant enzyme from Malayan pit viper (Agkistrodon rhodostoma) venom. Meth Enzymol 1976; 45B: 205-13.
27. Stocker K, Barlow GH. The coagulant enzyme from Bothrops atrox venom (batroxobin). Meth Enzymol 1976; 45B: 214-23.
28. Hung CC, Huang KF, Chiou SH. Characterization of one novel venom protease with beta fibrinogenase activity from the Taiwan habu (Trimeresurus mucrosquamatus): purification and cDNA sequence analysis. Biochem Biophys Res Commun 1994; 205: 1707-15.
29. Iwasaki A, Shieh TC, Shimohigashi Y, Waki M, Kihara H, Ohno M. Purification and characterization of a coagulant enzyme, okinaxobin I, from the venom of Trimeresurus okinavensis (Himehabu snake) which releases fibrinopeptide. B J Biochem 1990; 108: 822-8.
30. Faure G, Bon C. Crotoxin, a phospholipase A2 neurotoxin from the South American rattlesnake Crotalus durissus terrificus: purification of several isoforms and comparison of their molecular structure and of their biological activities. Biochemistry 1988; 27: 730-8.
31. Faure G, Guillaume J-L, Camoin L, Saliou B, Bon C. Multiplicity of acidic subunit isoforms of crotoxin, the phospholipase A2 neurotoxin from Crotalus durissus terrificus venom, results from posttranslational modifications. Biochemistry 1991; 30: 8074-83.
32. Tokunaga F, Nagasawa K, Tamura S, Miyata T, Iwanaga S, Kisiel W. The factor V-activating enzyme (RVV-V) from Russell's viper venom. Identification of isoproteins RVV-V alpha, -V beta, and V-gamma and their complete amino acid sequences. J Biol Chem 1988; 263: 17471-81.
33. Randolph A, Chamberlain SH, Chu H-LC, Retzios AD, Markland FS, Masiarz FR. Amino acid sequence of fibrolase, a direct-acting fibrinolytic enzyme from Agkistrodon contortrix contortrix venom. Protein Sci 1992; 1: 590-600.
34. Deshimaru M, Ogawa T, Nakashima K-I, Nobuhisa I, Chijiwa T, Shimohigashi Y, Furumaki Y, Niwa M, Yamashina I, Hattori S, Ohno M. Accelerated evolution of crotalinae snake venom gland serine proteases. FEBS Letters 1996; 397: 83-8.
35. Hahn B-S, Yang K-Y, Park E-M, Chang I-M, Kim Y-S. Purification and molecular cloning of calobin, a thrombin-like enzyme from Agkistrodon caliginosus (Korean viper). J Biochem 1996; 119: 835-43.
36. Shich T-C, Kawabata S-I, Kihara H, Ohno M, Iwanaga S. Amino acid sequence of a coagulant enzyme, flavoxobin, from Trimeresurus flavoviridis venom. J Biochem 1988, 103: 596-605.
37. Pirkle H, Theodor I, Lopez R. Catroxobin, a weakly thrombin-like enzyme from the venom of Crotalus atrox. NH2-terminal and active site amino acid sequences. Thromb Res 1989; 56: 159-68.
38. Pirkle H, Theodor I, Miyada D, Simmons G. Thrombin-like enzyme from the venom of Bitis gabonica. Purification, properties and coagulant actions. J Biol Chem 1986; 261: 8830-5.
39. Au L-C, Lin S-B, Chou J-S, Teh G-W, Chang K-J, Shih C-M. Molecular cloning and sequence analysis of the cDNA for ancrod, a thrombin-like enzyme from the venom of Calloselasma rhodostoma. Biochem J 1993; 294: 387-90.
40. Bode W, Mayr I, Baumann U, Huber R, Stone SR, Hofsteenge J. The refined 1.9 Å X-ray crystal structure of human α-thrombin: interaction with D-Phe-Pro-Arg chloromethylketone and significance of the Tyr-Pro-Pro-Trp insertion segment. Embo J 1989; 8: 3467-75.
41. Mandelbaum FR, Henriques OB. Purification and properties of Bothrops protease A. Arch Biochem Biophys 1964; 104: 369-73.