Identification of an ADAMTS-4 cleavage motif using phage display leads to the development of fluorogenic peptide substrates and reveals matrilin-3 as a novel substrate

Journal of Biological Chemistry

Volumn 282, Issue 15, 2007, Pages 11101-11109

  Hills, Robert ^a,c   Mazzarella, Richard ^a   Fok, Kam ^a   Liu, Min ^a   Nemirovskiy, Olga ^a   Leone, Joseph ^a   Zack, Marc D ^a   Arner, Elizabeth C ^a   Viswanathan, Malini ^b   Abujoub, Aida ^b   Muruganandam, Arumugam ^b   Sexton, Daniel J ^b   Bassill, Gary J ^b   Sato, Aaron K ^b   Malfait, Anne Marie ^a   Tortorella, Micky D ^a  

^a PFIZER GLOBAL RESEARCH AND DEVELOPMENT   (United States)

^b Dyax Corp   (United States)

^c NONE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CLEAVAGE SITES; FLUORESCENCE-BASED SUBSTRATES; PEPTIDE SUBSTRATES;

BINDING SITES; CATALYSIS; FLUORESCENCE; ISOMERIZATION; PEPTIDES; SUBSTRATES;

ENZYMES;

ADAMTS 4 PROTEASE; MATRILIN 3; PEPTIDE LIBRARY; PROTEINASE; UNCLASSIFIED DRUG; ADAM PROTEIN; AGGRECANASE 1; AGGRECANASE-1; GLUTAMIC ACID; ISOPROTEIN; PEPTIDE; PROCOLLAGEN N PROTEINASE; RECOMBINANT PROTEIN; SCLEROPROTEIN;

ARTICLE; CATALYSIS; ENZYME DEGRADATION; PHAGE DISPLAY; PRIORITY JOURNAL; AMINO ACID SEQUENCE; CHEMISTRY; ENZYME SPECIFICITY; GENETICS; IC 50; KINETICS; METABOLISM; MOLECULAR GENETICS; PROTEIN MICROARRAY; PROTEIN MOTIF; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY; SPECTROFLUOROMETRY; SYNTHESIS;

ADAM PROTEINS; AMINO ACID MOTIFS; AMINO ACID SEQUENCE; EXTRACELLULAR MATRIX PROTEINS; GLUTAMIC ACID; INHIBITORY CONCENTRATION 50; KINETICS; MOLECULAR SEQUENCE DATA; PEPTIDE LIBRARY; PEPTIDES; PROCOLLAGEN N-ENDOPEPTIDASE; PROTEIN ARRAY ANALYSIS; PROTEIN ISOFORMS; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, AMINO ACID; SPECTROMETRY, FLUORESCENCE; SUBSTRATE SPECIFICITY;

EID: 34249681972     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M611588200     Document Type: Article

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