Improved single-chain transactivators of the Tet-On gene expression system

BMC Biotechnology

Volumn 7, Issue , 2007, Pages

  Zhou, Xue ^a   Symons, Jori ^a   Hoppes, Rieuwert ^a   Krueger, Christel ^b   Berens, Christian ^b   Hillen, Wolfgang ^b   Berkhout, Ben ^a   Das, Atze T ^a  

^a ACADEMIC MEDICAL CENTER   (Netherlands)

^b UNIVERSITY OF ERLANGEN NUREMBERG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

EUKARYOTES; GENE REGULATORY SYSTEMS; MULTIPLE GENES; MUTATION;

AMINO ACIDS; CELLS; DNA; GENE EXPRESSION; PROTEINS;

GENETIC ENGINEERING;

DOXYCYCLINE; HETERODIMER; TET OFF PROTEIN; TET ON PROTEIN; TRANSACTIVATOR PROTEIN; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ARTICLE; BINDING SITE; CONTROLLED STUDY; GENE CONTROL; GENE MUTATION; GENETIC VARIABILITY; HUMAN; NONHUMAN; PROTEIN EXPRESSION; REPRESSOR GENE;

GENE EXPRESSION; GENE EXPRESSION REGULATION; PROMOTER REGIONS, GENETIC; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; REPRESSOR PROTEINS; TRANSCRIPTIONAL ACTIVATION; UP-REGULATION;

EUKARYOTA;

EID: 33847181641     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-7-6     Document Type: Article

References (34)

1. Berens C Hillen W Gene regulation by tetracyclines. Constraints of resistance regulation in bacteria shape TetR for application in eukaryotes Eur J Biochem 2003 270 3109-3121 10.1046/ j.1432-1033.2003.03694.x 12869186
2. Gossen M Bujard H Tetracyclines in the control of gene expression in eukaryotes Tetracyclines in biology, chemistry and medicine Basel, Birkhäuser Verlag Nelson M, Hillen W and Greenwald RA 2001 139-157
3. Baron U Bujard H Tet repressor-based system for regulated gene expression in eukaryotic cells: Principles and advances Methods Enzymol 2000 327 401-421 11044999
4. Gossen M Bujard H Tight control of gene expression in mammalian cells by tetracycline-responsive promoters Proc Natl Acad Sci USA 1992 89 5547-5551 49329 1319065 10.1073/pnas.89.12.5547
5. Gossen M Freundlieb S Bender G Müller G Hillen W Bujard H Transcriptional activation by tetracyclines in mammalian cells Science 1995 268 1766-1769 10.1126/science.7792603 7792603
6. Urlinger S Baron U Thellmann M Hasan MT Bujard H Hillen W Exploring the sequence space for tetracycline-dependent transcriptional activators: novel mutations yield expanded range and sensitivity Proc Natl Acad Sci USA 2000 97 7963-7968 16653 10859354 10.1073/pnas.130192197
7. Deuschle U Meyer WK Thiesen HJ Tetracycline-reversible silencing of eukaryotic promoters Mol Cell Biol 1995 15 1907-1914 230416 7891684
8. Forster K Helbl V Lederer T Urlinger S Wittenburg N Hillen W Tetracycline-inducible expression systems with reduced basal activity in mammalian cells Nucleic Acids Res 1999 27 708-710 148237 9863002 10.1093/ nar/27.2.708
9. Freundlieb S Schirra-Müller C Bujard H A tetracycline controlled activation/repression system with increased potential for gene transfer into mammalian cells J Gene Med 1999 1 4-12 10.1002/ (SICI)1521-2254(199901/02)1:1<4::AID-JGM4>3.0.CO;2-Y 10738580
10. Rossi FM Guicherit OM Spicher A Kringstein AM Fatyol K Blakely BT Blau HM Tetracycline-regulatable factors with distinct dimerization domains allow reversible growth inhibition by p16 Nat Genet 1998 20 389-393 10.1038/3871 9843215
11. Krueger C Danke C Pfleiderer K Schuh W Jack HM Lochner S Gmeiner P Hillen W Berens C A gene regulation system with four distinct expression levels J Gene Med 2006 8 1037-1047 10.1002/jgm.932 16779863
12. Helbl V Hillen W Stepwise selection of TetR variants recognizing tet operator 4C with high affinity and specificity J Mol Biol 1998 276 313-318 10.1006/jmbi.1997.1540 9512703
13. Helbl V Tiebel B Hillen W Stepwise selection of TetR variants recognizing tet operator 6C with high affinity and specificity J Mol Biol 1998 276 319-324 10.1006/jmbi.1997.1539 9512704
14. Henssler EM Scholz O Lochner S Gmeiner P Hillen W Structure-based design of Tet repressor to optimize a new inducer specificity Biochemistry 2004 43 9512-9518 10.1021/bi049682j 15260494
15. Scholz O Köstner M Reich M Gastiger S Hillen W Teaching TetR to recognize a new inducer J Mol Biol 2003 329 217-227 10.1016/ S0022-2836(03)00427-3 12758071
16. Berens C Lochner S Löber S Usai I Schmidt A Drueppel L Hillen W Gmeiner P Subtype selective tetracycline agonists and their application for a two-stage regulatory system Chembiochem 2006 7 1320-1324 10.1002/ cbic.200600226 16871602
17. Krueger C Schmidt A Danke C Hillen W Berens C Transactivator mutants with altered effector specificity allow selective regulation of two genes by tetracycline variants Gene 2004 331 125-131 10.1016/ j.gene.2004.02.006 15094198
18. Krueger C Berens C Schmidt A Schnappinger D Hillen W Single-chain Tet transregulators Nucleic Acids Res 2003 31 3050-3056 162254 12799431 10.1093/nar/gkg421
19. Ameres SL Drueppel L Pfleiderer K Schmidt A Hillen W Berens C Inducible DNA-loop formation blocks transcriptional activation by an SV40 enhancer EMBO J 2005 24 358-367 545818 15650749 10.1038/sj.emboj.7600531
20. Drueppel L Pfleiderer K Schmidt A Hillen W Berens C A short autonomous repression motif is located within the N-terminal domain of CTCF FEBS Lett 2004 572 154-158 10.1016/j.febslet.2004.07.027 15304340
21. Verhoef K Marzio G Hillen W Bujard H Berkhout B Strict control of human immunodeficiency virus type 1 replication by a genetic switch: Tet for Tat J Virol 2001 75 979-987 113994 11134311 10.1128/JVI.75.2.979-987.2001
22. Das AT Verhoef K Berkhout B A conditionally replicating virus as a novel approach toward an HIV vaccine Methods Enzymol 2004 388 359-379 15289083
23. Marzio G Verhoef K Vink M Berkhout B In vitro evolution of a highly replicating, doxycycline-dependent HIV for applications in vaccine studies Proc Natl Acad Sci USA 2001 98 6342-6347 33470 11353837 10.1073/ pnas.111031498
24. Das AT Zhou X Vink M Klaver B Verhoef K Marzio G Berkhout B Viral evolution as a tool to improve the tetracycline-regulated gene expression system J Biol Chem 2004 279 18776-18782 10.1074/ jbc.M313895200 14761948
25. Zhou X Vink M Klaver B Verhoef K Marzio G Das AT Berkhout B The genetic stability of a conditional-live HIV-1 variant can be improved by mutations in the Tet-On regulatory system that restrain evolution J Biol Chem 2006 281 17084-17091 10.1074/jbc.M513400200 16627480
26. Zhou X Vink M Klaver B Berkhout B Das AT Optimization of the Tet-On system for regulated gene expression through viral evolution Gene Ther 2006 13 1382-1390 10.1038/sj.gt.3302780 16724096
27. Zhou X Vink M Berkhout B Das AT Modification of the Tet-On regulatory system prevents the conditional-live HIV-1 variant from losing doxycycline-control Retrovirology 2006 3 82 1637113 17094796 10.1186/ 1742-4690-3-82
28. Baron U Gossen M Bujard H Tetracycline-controlled transcription in eukaryotes: Novel transactivators with graded transactivation potential Nucleic Acids Res 1997 25 2723-2729 146828 9207017 10.1093/nar/25.14.2723
29. Baron U Schnappinger D Helbl V Gossen M Hillen W Bujard H Generation of conditional mutants in higher eukaryotes by switching between the expression of two genes Proc Natl Acad Sci USA 1999 96 1013-1018 15342 9927685 10.1073/pnas.96.3.1013
30. Müller G Hecht B Helbl V Hinrichs W Saenger W Hillen W Characterization of non-inducible Tet repressor mutants suggests conformational changes necessary for induction Nat Struct Biol 1995 2 693-703 10.1038/nsb0895-693 7552732
31. Takahashi M Degenkolb J Hillen W Determination of the equilibrium association constant between Tet repressor and tetracycline at limiting Mg2+ concentrations: A generally applicable method for effector-dependent high-affinity complexes Anal Biochem 1991 199 197-202 10.1016/0003-2697(91)90089-C 1812784
32. Mikaelian I Sergeant A A general and fast method to generate multiple site directed mutations Nucleic Acids Res 1992 20 376 310390 1311073 10.1093/nar/20.2.376
33. Auersperg N Long-term cultivation of hypodiploid human tumor cells J Natl Cancer Inst 1964 32 135-163 14114965
34. Ruijter JM Thygesen HH Schoneveld OJ Das AT Berkhout B Lamers WH Factor correction as a tool to eliminate between-session variation in replicate experiments: Application to molecular biology and retrovirology Retrovirology 2006 3 2 1368993 16398936 10.1186/1742-4690-3-2