Optimization of the Tet-On system for regulated gene expression through viral evolution

Gene Therapy

Volumn 13, Issue 19, 2006, Pages 1382-1390

  Zhou, X ^a   Vink, M ^a   Klaver, B ^a   Berkhout, B ^a   Das, A T ^a  

^a UNIVERSITY OF AMSTERDAM   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

DOXYCYCLINE; MINOCYCLINE; RETROVIRUS VECTOR; TETRACYCLINE;

ARTICLE; CONTROLLED STUDY; ESCHERICHIA COLI; GENE EXPRESSION REGULATION; GENE EXPRESSION SYSTEM; GENE MUTATION; GENETIC VARIABILITY; HUMAN; HUMAN CELL; HUMAN IMMUNODEFICIENCY VIRUS 1; MAMMAL CELL; NONHUMAN; PRIORITY JOURNAL; PROCESS OPTIMIZATION; PROMOTER REGION; TET ON SYSTEM; TETRACYCLINE REGULATED GENE EXPRESSION SYSTEM; TRANSCRIPTION INITIATION; VIRUS CULTURE; VIRUS GENOME; VIRUS REPLICATION;

ANTI-BACTERIAL AGENTS; BIOLOGICAL EVOLUTION; BLOTTING, WESTERN; CELL LINE; CLONING, MOLECULAR; COMBINED MODALITY THERAPY; DNA; DOXYCYCLINE; ESCHERICHIA COLI; EVOLUTION; GENE EXPRESSION; GENE EXPRESSION REGULATION, VIRAL; GENE THERAPY; GENETIC ENGINEERING; HELA CELLS; HIV-1; HUMANS; MUTATION; PROTEINS; T-LYMPHOCYTES; TETRACYCLINE; TRANS-ACTIVATORS; TRANSGENES; VIRUS REPLICATION;

ESCHERICHIA COLI; HUMAN IMMUNODEFICIENCY VIRUS 1; MAMMALIA;

EID: 33748854037     PISSN: 09697128     EISSN: 14765462     Source Type: Journal    
DOI: 10.1038/sj.gt.3302780     Document Type: Article

References (37)

1. Fussenegger M. The impact of mammalian gene regulation concepts on functional genomic research, metabolic engineering, and advanced gene therapies. Biotechnol Prog 2001; 17: 1-51.
2. Baron U, Bujard H. Tet repressor-based system for regulated gene expression in eukaryotic cells: Principles and advances. Methods Enzymol 2000; 327: 401-421.
3. Gossen M, Bujard H. Tetracyclines in the control of gene expression in eukaryotes. In: Nelson M, Hillen W, Greenwald RA (eds). Tetracyclines in Biology, Chemistry and Medicine. Birkhäuser Verlag: Basel, 2001, pp 139-157.
4. Berens C, Hillen W. Gene regulation by tetracyclines. Constraints of resistance regulation in bacteria shape TetR for application in eukaryotes. Eur J Biochem 2003; 270: 3109-3121.
5. Gossen M, Bujard H. Tight control of gene expression in mammalian cells by tetracycline-responsive promoters. Proc Natl Acad Sci USA 1992; 89: 5547-5551.
6. Gossen M, Freundlieb S, Bender G, Muller G, Hillen W, Bujard H. Transcriptional activation by tetracyclines in mammalian cells. Science 1995; 268: 1766-1769.
7. Mohammadi S, Alvarez-Vallina L, Ashworth LJ, Hawkins RE. Delay in resumption of the activity of tetracycline-regulatable promoter following removal of tetracycline analogues. Gene Therapy 1997; 4: 993-997.
8. Mizuguchi H, Hayakawa T. The tet-off system is more effective than the tet-on system for regulating transgene expression in a single adenovirus vector. J Gene Med 2002; 4: 240-247.
9. Vogel R, Amar L, Thi AD, Saillour P, Mallet J. A single lentivirus vector mediates doxycycline-regulated expression of transgenes in the brain. Hum Gene Ther 2004; 15: 157-165.
10. Baron U, Gossen M, Bujard H. Tetracycline-controlled transcription in eukaryotes: Novel transactivators with graded transactivation potential. Nucleic Acids Res 1997; 25: 2723-2729.
11. Baron U, Schnappinger D, Helbl V, Gossen M, Hillen W, Bujard H. Generation of conditional mutants in higher eukaryotes by switching between the expression of two genes. Proc Natl Acad Sci USA 1999; 96: 1013-1018.
12. Urlinger S, Baron U, Thellmann M, Hasan MT, Bujard H, Hillen W. Exploring the sequence space for tetracycline-dependent transcriptional activators: Novel mutations yield expanded range and sensitivity. Proc Natl Acad Sci USA 2000; 97: 7963-7968.
13. Das AT, Zhou X, Vink M, Klaver B, Verhoef K, Marzio G et al. Viral evolution as a tool to improve the tetracycline-regulated gene expression system. J Biol Chem 2004; 279: 18776-18782.
14. Verhoef K, Marzio G, Hillen W, Bujard H, Berkhout B. Strict control of human immunodeficiency virus type 1 replication by a genetic switch: Tet for Tat. J Virol 2001; 75: 979-987.
15. Das AT, Verhoef K, Berkhout B. A conditionally replicating virus as a novel approach toward an HIV vaccine. Methods Enzymol 2004; 388: 359-379.
16. Marzio G, Verhoef K, Vink M, Berkhout B. In vitro evolution of a highly replicating, doxycycline-dependent HIV for applications in vaccine studies. Proc Natl Acad Sci USA 2001; 98: 6342-6347.
17. Marzio G, Vink M, Verhoef K, de Ronde A, Berkhout B. Efficient human immunodeficiency virus replication requires a fine-tuned level of transcription. J Virol 2002; 76: 3084-3088.
18. Smith SM, Khoroshev M, Marx PA, Orenstein J, Jeang KT. Constitutively dead, conditionally live HIV-1 genomes. Ex vivo implications for a live virus vaccine. J Biol Chem 2001; 276: 32184-32190.
19. Berkhout B, Verhoef K, Marzio G, Klaver B, Vink M, Zhou X et al. Conditional virus replication as an approach to a safe live attenuated human immunodeficiency virus vaccine. J Neurovirol 2002; 8 (Suppl 2): 134-137.
20. Das AT, Zhou X, Vink M, Klaver B, Berkhout B. Conditional live virus as a novel approach towards a safe live attenuated HIV vaccine. Expert Rev Vaccines 2002; 1: 293-301.
21. Hinrichs W, Kisker C, Duvel M, Muller A, Tovar K, Hillen W et al. Structure of the Tet repressor-tetracycline complex and regulation of antibiotic resistance. Science 1994; 264: 418-420.
22. Kisker C, Hinrichs W, Tovar K, Hillen W, Saenger W. The complex formed between Tet repressor and tetracycline-Mg2+ reveals mechanism of antibiotic resistance. J Mol Biol 1995; 247: 260-280.
23. Orth P, Cordes F, Schnappinger D, Hillen W, Saenger W, Hinrichs W. Conformational changes of the Tet repressor induced by tetracycline trapping. J Mol Biol 1998; 279: 439-447.
24. Orth P, Schnappinger D, Hillen W, Saenger W, Hinrichs W. Structural basis of gene regulation by the tetracycline inducible Tet repressor-operator system. Nat Struct Biol 2000; 7: 215-219.
25. Henssler EM, Scholz O, Lochner S, Gmeiner P, Hillen W. Structure-based design of Tet repressor to optimize a new inducer specificity. Biochemistry 2004; 43: 9512-9518.
26. Scholz O, Kintrup M, Reich M, Hillen W. Mechanism of Tet repressor induction by tetracyclines: Length compensates for sequence in the alpha8-alpha9 loop. J Mol Biol 2001; 310: 979-986.
27. Muller G, Hecht B, Helbl V, Hinrichs W, Saenger W, Hillen W. Characterization of non-inducible Tet repressor mutants suggests conformational changes necessary for induction. Nat Struct Biol 1995; 2: 693-703.
28. Schubert P, Schnappinger D, Pfleiderer K, Hillen W. Identification of a stability determinant on the edge of the Tet repressor four-helix bundle dimerization motif. Biochemistry 2001; 40: 3257-3263.
29. Smith SD, Shatsky M, Cohen PS, Warnke R, Link MP, Glader BE. Monoclonal antibody and enzymatic profiles of human malignant T-lymphoid cells and derived cell lines. Cancer Res 1984; 44 (Part 1): 5657-5660.
30. Auersperg N. Long-term cultivation of hypodiploid human tumor cells. J Natl Cancer Inst 1964; 32: 135-163.
31. Das AT, Klaver B, Klasens BI, van Wamel JL, Berkhout B. A conserved hairpin motif in the R-U5 region of the human immunodeficiency virus type 1 RNA genome is essential for replication. J Virol 1997; 71: 2346-2356.
32. Mikaelian I, Sergeant A. A general and fast method to generate multiple site directed mutations. Nucleic Acids Res 1992; 20: 376.
33. Das AT, Klaver B, Berkhout B. A hairpin structure in the R region of the human immunodeficiency virus type 1 RNA genome is instrumental in polyadenylation site selection. J Virol 1999; 73: 81-91.
34. Ruijter JM, Thygesen HH, Schoneveld OJ, Das AT, Berkhout B, Lamers WH. Factor correction as a tool to eliminate between-session variation in replicate experiments:aPplication to molecular biology and retrovirology. Retrovirology 2006; 3: 2.
35. Krueger C, Berens C, Schmidt A, Schnappinger D, Hillen W. Single-chain Tet transregulators. Nucleic Acids Res 2003; 31: 3050-3056.
36. Kraulis PJ. MOLSCRIPT: A program to produce both detailed and schematic plots of protein structures. J Appl Crystallogr 1991; 24: 946-950.
37. Merritt EA, Bacon DJ. Raster3D: Photorealistic molecular graphics. Methods Enzymol 1997; 277: 505-524.