Structure of the 70S ribosome complexed with mRNA and tRNA

Science

Volumn 313, Issue 5795, 2006, Pages 1935-1942

  Selmer, Maria ^a   Dunham, Christine M ^a   Murphy IV, Frank V ^a   Weixlbaumer, Albert ^a   Petry, Sabine ^a   Kelley, Ann C ^a   Weir, John R ^a   Ramakrishnan, V ^a  

^a MRC LABORATORY OF MOLECULAR BIOLOGY   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

CRYSTAL STRUCTURE; GENETIC ENGINEERING; INTERFACES (MATERIALS); IONS;

ARCHAEAL RIBOSOME; MESSENGER RNA (MRNA); METAL IONS; RIBOSOME;

RNA;

MAGNESIUM ION; MESSENGER RNA; PAROMOMYCIN; PEPTIDYLTRANSFERASE; TRANSFER RNA;

CRYSTAL STRUCTURE; ION; RNA; SPATIAL RESOLUTION; STABILIZATION;

70S RIBOSOME SUBUNIT; ARCHAEBACTERIUM; ARTICLE; CONFORMATIONAL TRANSITION; CRYOELECTRON MICROSCOPY; CRYSTAL STRUCTURE; DEINOCOCCUS RADIODURANS; ESCHERICHIA COLI; FOURIER ANALYSIS; HALOARCULA; HYDROGEN BOND; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; RIBOSOME SUBUNIT; RNA TRANSLATION; THERMUS THERMOPHILUS;

ANTICODON; BACTERIAL PROTEINS; CODON; CRYSTALLIZATION; CRYSTALLOGRAPHY, X-RAY; MAGNESIUM; MODELS, MOLECULAR; NUCLEIC ACID CONFORMATION; PEPTIDYL TRANSFERASES; PROTEIN BIOSYNTHESIS; PROTEIN CONFORMATION; RIBOSOMAL PROTEINS; RIBOSOMES; RNA, BACTERIAL; RNA, MESSENGER; RNA, TRANSFER; RNA, TRANSFER, MET; RNA, TRANSFER, PHE; THERMUS THERMOPHILUS;

ARCHAEA; BACTERIA (MICROORGANISMS);

EID: 33749354360     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.1131127     Document Type: Article

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48. Crystals were screened at Daresbury Labs, UK, or at European Synchrotron Radiation Facility (ESRF), Grenoble, France. Data were collected at the Swiss Light Source, Paul Scherrer Institut, Villigen, Switzerland, and at ESRF. We thank C. Schulze-Briese and R. Ravelli for help and advice with data collection; W. Kabsch for advice with the data integration program XDS; P. Adams for advice and for modifying the refinement program CNS; D. Gohara for optimally compiling an operating system X version of this modified CNS; P. Emsley for advice with the graphics program COOT; K. Nierhaus and E. Schmitt for gifts of overproducing clones of Phe- and fMet-tRNAs, respectively; and T. M. Schmeing for critical comments. This work was supported by the Medical Research Council (UK), NIH grant GM67624, the Agouron Institute and fellowships from the Wenner-Gren Foundations (M.S.), the American Cancer Society (C.M.D.), European Molecular Biology Organization (F.V.M.), Austrian Academy of Sciences (A.W.), and the Boehringer-lngelheim Fond (S.P.). Coordinates and structure factors have been deposited with the Protein Data Bank (PDB) with accession codes 2j00 (305-1), 2j01 (505-1), 2j02 (305-2), and 2j03 (505-2). V.R. holds stock options in and is on the Scientific Advisory Board of Rib-X Pharmaceuticals, a company that develops antibacterial drugs that target the ribosome.