Expression and functional purification of a glycosylation deficient version of the human adenosine 2a receptor for structural studies

Protein Expression and Purification

Volumn 49, Issue 1, 2006, Pages 129-137

  Fraser, Niall J ^a  

^a UNIVERSITY OF GLASGOW   (United Kingdom)

Author keywords

Adenosine 2a receptor; Expression; G protein coupled receptor; Membrane protein; Pichia pastoris; Purification; Structure determination

Indexed keywords

ADENOSINE A2A RECEPTOR; HYBRID PROTEIN;

ARTICLE; CELL MEMBRANE; CHEMISTRY; CIRCULAR DICHROISM; GENE EXPRESSION; GENETIC TRANSFORMATION; GENETICS; GLYCOSYLATION; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; PLASMID; PROTEIN SECONDARY STRUCTURE; SOLUBILITY;

CELL MEMBRANE; CIRCULAR DICHROISM; CLONING, MOLECULAR; GENE EXPRESSION; GLYCOSYLATION; HUMANS; PLASMIDS; PROTEIN STRUCTURE, SECONDARY; RECEPTOR, ADENOSINE A2A; RECOMBINANT FUSION PROTEINS; SOLUBILITY; TRANSFORMATION, GENETIC;

PICHIA PASTORIS;

EID: 33747780439     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2006.03.006     Document Type: Article

References (33)

1. Bockaert J., and Pin J.P. Molecular tinkering of G-protein-coupled receptors: an evolutionary success. EMBO J. 18 (1999) 1723-1729
2. Unger V.M., Hargrave P.A., Baldwin J.M., and Schertler G.F.X. Arrangement of rhodopsin transmembrane alpha-helices. Nature 389 (1997) 203-206
3. Palczewski K., Kumasaka T., Hori T., Behnke C.A., Motoshima H., Fox B.A., Le Trong I., Teller D.C., Okada T., Stenkamp R.E., Yamamoto M., and Miyano M. Crystal structure of rhodopsin: a G-protein-coupled receptor. Science 289 (2000) 739-745
4. Edwards P.C., Li J., Burghammer M., McDowell J.H., Villa C., Hargrave P.A., and Schertler G.F.X. Crystals of native and modified bovine rhodopsins and their heavy atom derivatives. J. Mol. Biol. 343 (2004) 1439-1450
5. Li J., Edwards P.C., Burghammer M., Villa C., and Schertler G.F.X. Structure of bovine rhodopsin in a trigonal crystal form. J. Mol. Biol. 343 (2004) 1409-1438
6. Baneres J.L., Martin A., Hullot P., Girard J.P., Rossi J.C., and Parello J. Structure-based analysis of GPCR function: conformational adaptation of both agonist and receptor upon leukotriene B4 binding to recombinant BLT1. J. Mol. Biol. 329 (2003) 801-814
7. Baneres J.L., Mesnier D., Martin A., Joubert L., Dumuis A., and Bockaert J. Molecular characterization of a purified 5-HT4 receptor. J. Biol. Chem. 280 (2005) 20253-20260
8. Tucker J., and Grisshammer R. Purification of a rat neurotensin receptor expressed in Escherichia coli. Biochem. J. 317 (1996) 891-899
9. 2A receptor functionally expressed in Escherichia coli. Eur. J. Biochem. 269 (2002) 82-91
10. White J.F., Trinh L.B., Shiloach J., and Grisshammer R. Automated large-scale purification of a G protein-coupled receptor for neurotensin. FEBS Lett. 564 (2004) 289-293
11. Andersen B., and Stevens R.C. The human D1A dopamine receptor : heterologous expression in Saccharomyces cerevisiae and purification of the functional receptor. Protein Expr. Purif. 13 (1998) 111-119
12. Weiss H.M., Haase W., Michel H., and Reilander H. Expression of functional mouse 5-HT5A serotonin receptor in the methylotrophic yeast Pichia pastoris: pharmacological characterisation and localisation. FEBS Lett. 337 (1995) 451-456
13. 2-adrenergic receptor produced in the methylotrophic yeast Pichia pastoris. Biochem. J. 330 (1998) 1137-1147
14. Schiller H., Haase W., Molsberger E., Janssen P., Michel H., and Reilander H. The human ETB endothelin receptor heterologously produced in the methylotrophic yeast Pichia pastoris shows high-affinity binding and induction of stacked membranes. Receptor Channel 7 (2000) 93-107
15. Schiller H., Molsberger E., Janssen P., Michel H., and Reilander H. Solubilisation and purification of the human ETB endothelin receptor produced by high-level fermentation in Pichia pastoris. Receptor Channel 7 (2001) 453-469
16. Sarramegna V., Demange P., Milon A., and Talmont F. Optimising functional versus total expression of the human mu-opioid receptor in Pichia pastoris. Protein Expr. Purif. 24 (2002) 212-220
17. Feng W., Cai H., Pierce W.M., and Song Z.H. Expression of CB2 cannabinoid receptor in Pichia pastoris. Protein Expr. Purif. 26 (2002) 496-505
18. De Jong L.A.A., Grunewald S., Franke J.P., Uges D.R.A., and Bischoff R. Purification and characterisation of the recombinant human dopamine D2S receptor from Pichia pastoris. Protein Expr. Purif. 33 (2004) 176-184
19. Kim T.K., Zhang R.D., Feng W., Cai H., Pierce W., and Song Z.H. Expression and characterisation of human CB1 cannabinoid receptor in methylotropic yeast Pichia pastoris. Protein Expr. Purif. 40 (2005) 60-70
20. Massotte D., Baroche L., Simonin F., Yu L., Kieffer B., and Pattus F. Characterization of delta, kappa, and mu human opioid receptors overexpressed in baculovirus-infected cells. J. Biol. Chem. 272 (1997) 19987-19992
21. Warne T., Chirnside J., and Schertler G.F.X. Expression and purification of truncated, non-glycosylated turkey beta-adrenergic receptors for crystallization. Biochim. Biophys. Acta 1610 (2003) 133-140
22. Perret B.G., Wagner R., Lecat S., Brillet K., Rabut G., Bucher B., and Pattus F. Expression of EGFP-amino-tagged human mu opioid receptor in Drosophila Schneider 2 cells : a potential expression system for large-scale production of G-protein coupled receptors. Protein Expr. Purif. 31 (2003) 123-132
23. Ratnala V.R.P., Swarts H.G.P., van Oostrum J., Leurs R., de Groot H.J.M., Bakker R.A., and de Grip W.J. Large-scale overproduction, functional purification and ligand affinities of the His-tagged human histamine H1 receptor. Eur. J. Biochem. 271 (2004) 2636-2646
24. Lundström K. Semliki Forest virus vectors for rapid and high-level expression of integral membrane proteins. Biochim. Biophys. Acta - Biomembranes 1610 (2003) 90-96
25. Ohta A., and Sitkovsky M. Role of G protein-coupled adenosine receptors in downregulation of inflammation and protection from tissue damage. Nature 414 (2001) 916-920
26. Sitkovsky M.V., Lukashev D., Apasov S., Kojima H., Koshiba M., Caldwell C., Ohta A., and Thiel M. Physiological control of immune response and inflammatory tissue damage by hypoxia-inducible factors and adenosine A2A receptors. Annu. Rev. Immunol. 22 (2004) 657-682
27. Sreerama N., and Woody R.W. Estimation of protein secondary structure from CD spectra: Comparison of CONTIN, SELCON, and CDSSTR methods with an expanded reference set. Anal. Biochem. 287 (2000) 252-260
28. 2a adenosine receptors. Biochem. Pharmacol. 60 (2000) 661-668
29. 50) of an enzymatic reaction. Biochem. Pharmacol. 22 (1973) 3099-3108
30. 3H]ZM241385-an antagonist radioligand for adenosine A2a receptors in rat brain. Eur. J. Pharmacol. 411 (2001) 205-210
31. 2a adenosine receptor. Mol. Pharmacol. 48 (1995) 970-974
32. Ladds G., Goddard A., and Davey J. Functional analysis of heterologous GPCR signalling pathways in yeast. Trends Biotechnol. 23 (2005) 367-373
33. Linden J. Molecular approach to adenosine receptors : receptor-mediated mechanisms of tissue protection. Ann. Rev. Pharmacol. Toxicol. 41 (2001) 775-787