Cloning, comparative characterization of porcine SCAP gene, and identification of its two splice variants

Molecular Genetics and Genomics

Volumn 276, Issue 2, 2006, Pages 187-196

  Qiu, Huan ^a   Xia, Tao ^a   Chen, Xiaodong ^a   Zhao, Xuelian ^a   Gan, Li ^a   Feng, Shengqiu ^a   Lei, Ting ^a   Yang, Zaiqing ^a  

^a HUAZHONG AGRICULTURAL UNIVERSITY   (China)

Author keywords

Intron gain; Intron loss; Radiation hybridization mapping; Splicing variants; SREBP

Indexed keywords

AMINO ACID; COMPLEMENTARY DNA; PROTEIN; PROTEIN SCAP; UNCLASSIFIED DRUG;

ANIMAL TISSUE; ARTICLE; CHICKEN; CHROMOSOME 13Q; COW; DATA ANALYSIS; EXON; FAMILY; GENE DELETION; GENE IDENTIFICATION; GENE MAPPING; GENETIC VARIABILITY; GENOME; INTRON; LIPID METABOLISM; LIPID STORAGE; LIVER; MOLECULAR CLONING; MUSCLE TISSUE; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PRIORITY JOURNAL; PROTEIN FAMILY; SOMATIC CELL; SPECIES IDENTIFICATION; SWINE; TESTIS; UNINDEXED SEQUENCE; VERTEBRATE;

ALTERNATIVE SPLICING; ANIMALS; CHOLESTEROL; CHROMOSOME MAPPING; CHROMOSOMES; CLONING, MOLECULAR; EXONS; INTRACELLULAR SIGNALING PEPTIDES AND PROTEINS; INTRONS; LIPID METABOLISM; MEMBRANE PROTEINS; STEROL REGULATORY ELEMENT BINDING PROTEINS; SWINE;

RODENTIA; SUIDAE; SUS SCROFA; TAKIFUGU; TETRAODON; VERTEBRATA;

EID: 33746034601     PISSN: 16174615     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00438-006-0134-8     Document Type: Article

References (30)

1. Birney E, Andrews D, Caccamo M, Chen Y, Clarke L, Coates G, Cox T, Cunningham F, Curwen V, Cutts T, Down T, Durbin R, Fernandez-Suarez XM, Flicek P, Graf S, Hammond M, Herrero J, Howe K, Iyer V, Jekosch K, Kahari A, Kasprzyk A, Keefe D, Kokocinski F, Kulesha E, London D, Longden I, Melsopp C, Meidl P, Overduin B, Parker A, Proctor G, Prlic A, Rae M, Rios D, Redmond S, Schuster M, Sealy I, Searle S, Severin J, Slater G, Smedley D, Smith J, Stabenau A, Stalker J, Trevanion S, Ureta-Vidal A, Vogel J, White S, Woodwark C, Hubbard TJ (2006) Ensembl 2006. Nucleic Acids Res 34:D556-D561
2. Brambilla G, Cantafora A (2004) Metabolic and cardiovascular disorders in highly inbred lines for intensive pig farming: how animal welfare evaluation could improve the basic knowledge of human obesity. Ann Ist Super Sanita 40:241-244
3. Carver EA, Stubbs L (1997) Zooming in on the human-mouse comparative map: genome conservation re-examined on a high-resolution scale. Genome Res 7:1123-1137
4. Feiber AL, Rangarajan J, Vaughn JC (2002) The evolution of single-copy Drosophila nuclear 4f-rnp genes: spliceosomal intron losses create polymorphic alleles. J Mol Evol 55:401-413
5. Feramisco JD, Radhakrishnan A, Ikeda Y, Reitz J, Brown MS, Goldstein JL (2005) Intramembrane aspartic acid in SCAP protein governs cholesterol-induced conformational change. Proc Natl Acad Sci USA 102:3242-3247
6. Fink GR (1987) Pseudogenes in yeast? Cell 49:5-6
7. Goureau A, Yerle M, Schmitz A, Riquet J, Milan D, Pinton P, Frelat G, Gellin J (1996) Human and porcine correspondence of chromosome segments using bidirectional chromosome painting. Genomics 36:252-262
8. Horton JD, Goldstein JL, Brown MS (2002) SREBPs: activators of the complete program of cholesterol and fatty acid synthesis in the liver. J Clin Invest 109:1125-1131
9. Hughes AL, Todd BL, Espenshade PJ (2005) SREBP pathway responds to sterols and functions as an oxygen sensor in fission yeast. Cell 25:831-842
10. Jin S, Hu GA, Qian YH, Zhang L, Zhang J, Qiu G, Zeng QT, Gui JF (2005) Identification of one intron loss and phylogenetic evolution of Dfak gene in the Drosophila melanogaster species group. Genetica 125:223-230
11. Kuwabara PE, Labouesse M (2002) The sterol-sensing domain: multiple families, a unique role? Trends Genet 18:193-201
12. Loewen CJ, Levine TP (2002) Cholesterol homeostasis: not until the SCAP lady INSIGs. Curr Biol 12:R779-R781
13. Matsuda M, Korn BS, Hammer RE, Moon YA, Komuro R, Horton JD, Goldstein JL, Brown MS, Shimomura I (2001) SREBP cleavage-activating protein (SCAP) is required for increased lipid synthesis in liver induced by cholesterol deprivation and insulin elevation. Genes Dev 15:1206-1216
14. Milan D, Hawken R, Cabau C, Leroux S, Genet C, Lahbib Y, Tosser G, Robic A, Hatey F, Alexander L, Beattie C, Schook L, Yerle M, Gellin J (2000) IMpRH server: an RH mapping server available on the web. Bioinformatics 16:558-559
15. Nakajima T, Hamakubo T, Kodama T, Inazawa J, Emi M (1999) Genomic structure and chromosomal mapping of the human sterol regulatory element binding protein (SREBP) cleavage-activating protein (SCAP) gene. J Hum Genet 44:402-407
16. Neer EJ, Schmidt CJ, Nambudripad R, Smith TF (1994) The ancient regulatory-protein family of WD-repeat proteins. Nature 371:297-300
17. Nie T, Zhao XL, Qiu H, Xia T, Chen XD, Gan L, Feng SQ, Lei T, Dai MH, Yang ZQ (2005) Sequence analysis and map assignment of pig SREBF2 and ADFP. Anim Genet 36:455-457
18. Nohturfft A, Brown MS, Goldstein JL (1998) Topology of SREBP cleavage-activating protein, a polytopic membrane protein with a sterol-sensing domain. J Biol Chem 273:17243-17250
19. Osborne TF (2001) CREating a SCAP-less liver keeps SREBPs pinned in the ER membrane and prevents increased lipid synthesis in response to low cholesterol and high insulin. Genes Dev 15:1873-1878
20. Qiu H, Xia T, Chen XD, Feng SQ, Gan L, Lei T, Peng Y, Zhang GD, Nie T, Yue GP, Zhao XL, Yang ZQ (2005a) Sequencing and chromosome mapping of pig INSIG 2 and a related pseudogene. Anim Genet 36:188-189
21. Qiu H, Xia T, Chen XD, Gan L, Feng SQ, Lei T, Dai MH, Yang ZQ (2005b) Characterization of pig INSIG1 and assignment to SSC18. Anim Genet 36:284-286
22. Rawson RB, DeBose-Boyd R, Goldstein JL, Brown MS (1999) Failure to cleave sterol regulatory element-binding proteins (SREBPs) causes cholesterol auxotrophy in Chinese hamster ovary cells with genetic absence of SREBP cleavage-activating protein. J Biol Chem 274:28549-28556
23. Roy SW, Fedorov A, Gilbert W (2003) Large-scale comparison of intron positions in mammalian genes shows intron loss but no gain. Proc Natl Acad Sci USA 100:7158-7162
24. Seegmiller AC, Dobrosotskaya I, Goldstein JL, Ho YK, Brown MS, Rawson RB (2002) The SREBP pathway in Drosophila: regulation by palmitate, not sterols. Dev Cell 2:229-238
25. Wittenburg H, Lammert F, Wang DQ, Churchill GA, Li R, Bouchard G, Carey MC, Paigen B (2002) Interacting QTLs for cholesterol gallstones and gallbladder mucin in AKR and SWR strains of mice. Physiol Genomics 11:67-77
26. Yabe D, Brown MS, Goldstein JL (2002a) Insig-2, a second endoplasmic reticulum protein that binds SCAP and blocks export of sterol regulatory element-binding proteins. Proc Natl Acad Sci USA 99:12753-12758
27. Yabe D, Xia ZP, Adams CM, Rawson RB (2002b) Three mutations in sterol-sensing domain of SCAP block interaction with insig and render SREBP cleavage insensitive to sterols. Proc Natl Acad Sci USA 99:16672-16677
28. Yang T, Goldstein JL, Brown MS (2000) Overexpression of membrane domain of SCAP prevents sterols from inhibiting SCAP. SREBP exit from endoplasmic reticulum. J Biol Chem 275:29881-29886
29. Yang T, Espenshade PJ, Wright ME, Yabe D, Gong Y, Aebersold R, Goldstein JL, Brown MS (2002) Crucial step in cholesterol homeostasis: sterols promote binding of SCAP to INSIG-1, a membrane protein that facilitates retention of SREBPs in ER. Cell 23:489-500
30. Yerle M, Pinton P, Robic A, Alfonso A, Palvadeau Y, Delcros C, Hawken R, Alexander L, Beattie C, Schook L, Milan D, Gellin J (1998) Construction of a whole-genome radiation hybrid panel for high-resolution gene mapping in pigs. Cytogenet Cell Genet 82:182-188