Crucial step in cholesterol homeostasis: Sterols promote binding of SCAP to INSIG-1, a membrane protein that facilitates retention of SREBPs in ER

Cell

Volumn 110, Issue 4, 2002, Pages 489-500

  Yang, Tong ^a   Espenshade, Peter J ^a   Wright, Michael E ^b   Yabe, Daisuke ^a   Gong, Yi ^a   Aebersold, Ruedi ^b   Goldstein, Joseph L ^a   Brown, Michael S ^a  

^a UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

^b INSTITUTE FOR SYSTEMS BIOLOGY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

PROTEIN; PROTEIN INSIG 1; PROTEIN SCAP; UNCLASSIFIED DRUG;

ARTICLE; CHOLESTEROL SYNTHESIS; CONTROLLED STUDY; CORRELATION ANALYSIS; ENDOPLASMIC RETICULUM; GENE OVEREXPRESSION; GOLGI COMPLEX; HOMEOSTASIS; HUMAN; IMMUNOPRECIPITATION; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN BINDING; TANDEM MASS SPECTROMETRY;

EID: 0037162719     PISSN: 00928674     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0092-8674(02)00872-3     Document Type: Article

References (27)

1. Bergmann J.E. Using temperature-sensitive mutants of VSV to study membrane protein biogenesis. Methods Cell Biol. 32:1989;85-110.
2. Brown M.S., Goldstein J.L. A proteolytic pathway that controls the cholesterol content of membranes, cells, and blood. Proc. Natl. Acad. Sci. USA. 96:1999;11041-11048.
3. Brown M.S., Ye J., Rawson R.B., Goldstein J.L. Regulated intramembrane proteolysis. a control mechanism conserved from bacteria to humans Cell. 100:2000;391-398.
4. Brown A.J., Sun L., Feramisco J.D., Brown M.S., Goldstein J.L. Cholesterol addition to ER membranes alters conformation of SCAP, the SREBP escort protein that regulates cholesterol metabolism. Mol. Cell. in press:2002.
5. DeBose-Boyd R.A., Brown M.S., Li W.-P., Nohturfft A., Goldstein J.L., Espenshade P.J. Transport-dependent proteolysis of SREBP. relocation of site-1 protease from Golgi to ER obviates the need for SREBP transport to Golgi Cell. 99:1999;703-712.
6. Diamond R.H., Du K., Lee V.M., Mohn K.L., Haber B.A., Tewari D.S., Taub R. Novel delayed-early and highly insulin-induced growth response genes. identification of HRS, a potential regulator of alternative pre-mRNA splicing J. Biol. Chem. 268:1993;15185-15192.
7. Goldstein J.L., Rawson R.B., Brown M.S. Mutant mammalian cells as tools to delineate the sterol regulatory element-binding protein pathway for feedback regulation of lipid synthesis. Arch. Biochem. Biophys. 397:2002;139-148.
8. Hua X., Nohturfft A., Goldstein J.L., Brown M.S. Sterol resistance in CHO cells traced to point mutation in SREBP cleavage activating protein (SCAP). Cell. 87:1996;415-426. a.
9. Hua X., Sakai J., Brown M.S., Goldstein J.L. Regulated cleavage of sterol regulatory element binding proteins (SREBPs) requires sequences on both sides of the endoplasmic reticulum membrane. J. Biol. Chem. 271:1996;10379-10384. b.
10. Kyte J., Doolittle R.F. A simple method for displaying the hydropathic character of a protein. J. Mol. Biol. 157:1982;105-132.
11. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:1970;680-685.
12. Lippincott-Schwartz J., Yuan L.C., Bonifacino J.S., Klausner R.D. Rapid redistribution of Golgi proteins into the ER in cells treated with brefeldin A. evidence for membrane cycling from Golgi to ER Cell. 56:1989;801-813.
13. Luong, A. (2000). Identification of Three Novel SREBP-Activated Target Genes: Acetyl CoA Synthetase, 3-β-Hydroxysterol Dehydrogenase, and CL-6/INSIG1. Ph.D. Dissertation, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas, p. 1-137.
14. Metherall J.E., Goldstein J.L., Luskey K.L., Brown M.S. Loss of transcriptional repression of three sterol-regulated genes in mutant hamster cells. J. Biol. Chem. 264:1989;15634-15641.
15. Mohn K.L., Laz T.M., Hsu J.-C., Melby A.E., Bravo R., Taub R. The immediate-early growth response in regenerating liver and insulin-stimulated H-35 cells. comparison with serum-stimulated 3T3 cells and identification of 41 novel immediate-early genes Mol. Cell. Biol. 11:1991;381-390.
16. Nohturfft A., Brown M.S., Goldstein J.L. Sterols regulate processing of carbohydrate chains of wild-type SREBP cleavage-activating protein (SCAP), but not sterol-resistant mutants Y298C or D443N. Proc. Natl. Acad. Sci. USA. 95:1998;12848-12853. a.
17. Nohturfft A., Brown M.S., Goldstein J.L. Topology of SREBP cleavage-activating protein, a polytopic membrane protein with a sterol-sensing domain. J. Biol. Chem. 273:1998;17243-17250. b.
18. Nohturfft A., Yabe D., Goldstein J.L., Brown M.S., Espenshade P.J. Regulated step in cholesterol feedback localized to budding of SCAP from ER membranes. Cell. 102:2000;315-323.
19. Peng Y., Schwarz E.J., Lazar M.A., Genin A., Spinner N.B., Taub R. Cloning, human chromosomal assignment, and adipose and hepatic expression of the CL-6/INSIG1 gene. Genomics. 43:1997;278-284.
20. Rawson R.B., DeBose-Boyd R., Goldstein J.L., Brown M.S. Failure to cleave sterol regulatory element-binding proteins (SREBPs) causes cholesterol auxotrophy in Chinese hamster ovary cells with genetic absence of SREBP cleavage-activating protein. J. Biol. Chem. 274:1999;28549-28556.
21. Reeves P.J., Thurmond R.L., Khorana H.G. Structure and function in rhodopsin. high level expression of a synthetic bovine opsin gene and its mutants in stable mammalian cell lines Proc. Natl. Acad. Sci. USA. 93:1996;11487-11492.
22. Sakai J., Nohturfft A., Cheng D., Ho Y.K., Brown M.S., Goldstein J.L. Identification of complexes between the COOH-terminal domains of sterol regulatory element binding proteins (SREBPs) and SREBP cleavage-activating protein (SCAP). J. Biol. Chem. 272:1997;20213-20221.
23. Sakai J., Nohturfft A., Goldstein J.L., Brown M.S. Cleavage of sterol regulatory element binding proteins (SREBPs) at site-1 requires interaction with SREBP cleavage-activating protein. Evidence from in vivo competition studies. J. Biol. Chem. 273:1998;5785-5793.
24. Schagger H., Cramer W.A., von Jagow G. Analysis of molecular masses and oligomeric states of protein complexes by blue native electrophoresis and isolation of membrane protein complexes by two-dimensional native electrophoresis. Anal. Biochem. 217:1994;220-230.
25. Tolleshaug H., Goldstein J.L., Schneider W.J., Brown M.S. Posttranslational processing of the LDL receptor and its genetic disruption in familial hypercholesterolemia. Cell. 30:1982;715-724.
26. Yang T., Goldstein J.L., Brown M.S. Overexpression of membrane domain of SCAP prevents sterols from inhibiting SCAP/SREBP exit from endoplasmic reticulum. J. Biol. Chem. 275:2000;29881-29886.
27. ATP channels. Neuron. 22:1999;537-548.