Improved mutants from directed evolution are biased to orthologous substitutions

Protein Engineering, Design and Selection

Volumn 19, Issue 6, 2006, Pages 245-253

  Cochran, Jennifer R ^a,b,c   Kim, Yong Sung ^a,b,d   Lippow, Shaun M ^a,b   Rao, Balaji ^a,b   Wittrup, K Dane ^a,b,c  

^a MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

^b Massachusetts Institute of Technology Cambridge   (United States)

^c Stanford University   (United States)

^d Ajou University   (South Korea)

Author keywords

Epidermal growth factor; Epidermal growth factor receptor; In vitro evolution; Protein engineering; Yeast display

Indexed keywords

DNA; GENES; GROWTH KINETICS; MUTAGENESIS; PROTEINS; STATISTICAL METHODS; YEAST;

EPIDERMAL GROWTH FACTOR; EPIDERMAL GROWTH FACTOR RECEPTOR; IN VITRO EVOLUTION; PROTEIN ENGINEERING; YEAST DISPLAY;

LIVING SYSTEMS STUDIES;

EPIDERMAL GROWTH FACTOR; EPIDERMAL GROWTH FACTOR RECEPTOR; INTERLEUKIN 2; SUBTILISIN;

ARTICLE; DIRECTED MOLECULAR EVOLUTION; DNA SHUFFLING; GENE ISOLATION; GENE MUTATION; GENETIC VARIABILITY; HUMAN; MOLECULAR LIBRARY; NONHUMAN; ORTHOLOGY; PHYLOGENY; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN DOMAIN; RECEPTOR AFFINITY; SHOTGUN ORTHOLOG SCANNING MUTAGENESIS; SITE DIRECTED MUTAGENESIS; STATISTICAL ANALYSIS; WILD TYPE;

BASE SEQUENCE; BIOSENSING TECHNIQUES; DIRECTED MOLECULAR EVOLUTION; EPIDERMAL GROWTH FACTOR; FIBROBLASTS; GENE LIBRARY; HUMANS; INTERLEUKIN-2; MUTAGENESIS; MUTATION; PHYLOGENY; PROTEIN BINDING; RECEPTOR, EPIDERMAL GROWTH FACTOR; SUBTILISINS; TRANSFECTION; YEASTS;

EID: 33745726188     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzl006     Document Type: Article

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