Crystal structure of the motor domain of the human kinetochore protein CENP-E

Journal of Molecular Biology

Volumn 340, Issue 5, 2004, Pages 1107-1116

  Garcia Saez, Isabel ^a   Yen, Tim ^b   Wade, Richard H ^a   Kozielski, Frank ^a  

^a INSTITUT DE BIOLOGIE STRUCTURALE   (France)

^b FOX CHASE CANCER CENTER   (United States)

Author keywords

CENP E; CENP E, centromere associated protein E; crystal structure; EM, electron microscopy; kinesin; kinetochore; MAP, mitogen activated protein; MCAK, mitotic centromere associated kinesin; mitosis; MT, microtubule

Indexed keywords

ADENOSINE TRIPHOSPHATASE (MAGNESIUM); CENTROMERE PROTEIN E; KINESIN; UNCLASSIFIED DRUG;

ARTICLE; BINDING SITE; CENTROMERE; COMPARATIVE STUDY; CRYSTAL STRUCTURE; DRUG TARGETING; HUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN STRUCTURE;

EID: 3242771433     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jmb.2004.05.053     Document Type: Article

References (54)

1. Yen T.J., Schaar B.T. Kinetochore function: molecular motors, switches and gates. Curr. Biol. 8:1996;381-388
2. Rieder C.L., Salmon E.D. The vertebrate cell kinetochore and its role during mitotis. Trends Cell Biol. 8:1998;310-318
3. Grancell A., Sorger P.K. Kinetochores motor along. Curr. Biol. 8:1998;R382-R385
4. Kapoor T.M., Compton D.A. Searching for the middle ground: mechanisms of chromosome alignment during mitosis. J. Cell Biol. 157:2002;551-556
5. Cleveland D.W., Mao Y., Sullivan K.F. Centromers and kinetochores: from epigenetics to mitotic checkpoint signaling. Cell. 112:2003;407-421
6. Steuer E.R., Wordeman L., Schroer T.A., Sheetz M.P. Localization of cytoplasmic dynein to mitotic spindles and kinetochores. Nature. 345:1990;266-268
7. Pfarr C.M., Coue M., Grissom P.M., Hays T.S., Porter M.E., McIntosh J.R. Cytoplasmic dynein is localized to kinetochores during mitosis. Nature. 345:1990;263-265
8. Yen T.J., Compton D.A., Wise D., Zinkowski R.P., Brinkley B.R., Earnshaw W.C., Cleveland D.W. CENP-E, a novel human centromere-associated protein required for progression from metaphase to anaphase. EMBO J. 10:1991;1245-1254
9. Yen T.J., Li G., Schaar B.T., Szilak I., Cleveland D.W. CENP-E is a putative kinetochore motor that accumulates just before mitosis. Nature. 359:1992;536-539
10. Wordeman L., Mitchison T.J. Identification and partial characterization of mitotic centromere-associated kinesin, a kinesin-related protein that associates with centromeres during mitosis. J. Cell Biol. 128:1995;95-104
11. Wood K.W., Sakowicz R., Goldstein L.S., Cleveland D.W. CENP-E is a plus end-directed kinetochore motor required for metaphase chromosome alignment. Cell. 91:1997;357-366
12. Yucel J.K., Marszalek J.D., McIntosh J.R., Goldstein L.S., Cleveland D.W., Philp A.V. CENP-meta, an essential kinetochore kinesin required for the maintenance of metaphase chromosome alignment in Drosophila. J. Cell Biol. 150:2000;1-11
13. Miki M., Setou M., Kaneshiro K., Hirokawa N. All kinesin superfamily protein, KIF, genes in mouse and human. Proc. Natl Acad. Sci. USA. 98:2001;7004-7011
14. Dagenbach E.M., Endow S.A. A new kinesin tree. J. Cell Sci. 117:2004;3-7
15. Brown K.D., Coulson R.M., Yen T.J., Cleveland D.W. Cyclin-like accumulation and loss of the putative kinetochore motor CENP-E results from coupling continuous synthesis with specific degradation at the end of mitosis. J. Cell Biol. 125:1994;1303-1312
16. Brown K.D., Wood K.W., Cleveland D.W. The kinesin-like protein CENP-E is kinetochore-associated throughout poleward chromosome segregation during anaphase-A. J. Cell Sci. 109:1996;961-969
17. Schaar B.T., Chan G.K., Maddox P., Salmon E.D., Yen T.J. CENP-E function at kinetochores is essential for chromosome alignment. J. Cell Biol. 139:1997;1373-1382
18. Yao X., Abrieu A., Zheng Y., Sullivan K.F., Cleveland D.W. CENP-E forms a link between attachment of spindle microtubules to kinetochores and the mitotic checkpoint. Nature Cell Biol. 2:2000;484-491
19. Liu S.T., Chan G.K., Hittle J.C., Fujii G., Lees E., Yen T.J. Human MPS1 kinase is required for mitotic arrest induced by the loss of CENP-E from kinetochores. Mol. Biol. Cell. 14:2003;1638-1651
20. Weaver B.A., Bonday Z.Q., Putkey F.R., Kops G.J., Silk A.D., Cleveland D.W. Centromere-associated protein-E is essential for the mammalian mitotic checkpoint to prevent aneuploidy due to single chromosome loss. J. Cell Biol. 162:2003;551-563
21. McEwen B.F., Chan G.K., Zubrowski B., Savoian M.S., Sauer M.T., Yen T.J. CENP-E is essential for reliable bioriented spindle attachment, but chromosome alignment can be achieved via redundant mechanisms in mammalian cells. Mol. Biol. Cell. 12:2001;2776-2789
22. Liao H., Li G., Yen T.J. Mitotic regulation of microtubule cross-linking activity of CENP-E kinetochore protein. Science. 265:1994;394-398
23. Chan G.K., Schaar B.T., Yen T.J. Characterization of the kinetochore binding domain of CENP-E reveals interactions with the kinetochore proteins CENP-F and hBUBR1. J. Cell Biol. 143:1998;49-63
24. Zecevic M., Catling A.D., Eblen S.T., Renzi L., Hittle J.C., Yen T.J., et al. Active MAP kinase in mitosis: localization at kinetochores and association with the motor protein CENP-E. J. Cell Biol. 142:1998;1547-1558
25. Endow S.A., Fletterick R.J. Reversing a "backwards" motor. BioEssays. 20:1998;108-112
26. Woelke G., Schliwa M. Directional motility of kinesin motor proteins. Biochim. Biophys. Acta. 1496:2000;117-127
27. Thrower D.A., Jordan M.A., Wilson L. Modulation of CENP-E organization at kinetochores by spindle microtubule attachment. Cell Motil. Cytoskel. 35:1996;121-133
28. DeLuca J.G., Newton C.N., Himes R.H., Jordan M.A., Wilson L. Purification and characterization of native conventional kinesin, HSET, and CENP-E from mitotic HeLa cells. J. Biol. Chem. 276:2001;28014-28021
29. Hirel P.H., Schmitter M.J., Dessen P., Fayat G., Blanquet S. Extent of N-terminal methionine excision from Escherichia coli proteins is governed by the side-chain length of the penultimate amino acid. Proc. Natl Acad. Sci. USA. 85:1989;8247-8251
30. Kozielski F., Sack S., Marx A., Thormählen M., Biou V., Thompson A., et al. The crystal structure of dimeric kinesin and implications for microtubule-dependent motility. Cell. 91:1997;985-994
31. Kozielski F., De Bonis S., Buhrmeister W.P., Cohen-Addad C., Wade R.H. The crystal structure of the minus-end-directed microtubule motor protein ncd reveals variable dimer conformations. Structure. 7:1999;1407-1416
32. Sack S., Muller J., Marx A., Thormahlen M., Mandelkow E.M., Brady S.T., Mandelkow E. X-ray structure of motor and neck domains from rat brain kinesin. Biochemistry. 36:1997;16155-16165
33. Sindelar C.V., Budny M.J., Rice S., Naber N., Fletterick R., Cooke R. Two conformations in the human kinesin power stroke defined by X-ray crystallography and EPR spectroscopy. Nature Struct. Biol. 9:2002;844-848
34. Lombillo V.A., Stewart R.J., McIntosh J.R. Minus-end-directed motion of kinesin-coated microspheres driven by microtubule depolymerization. Nature. 373:1995;161-164
35. Turner J., Anderson R., Guo J., Beraud C., Fletterick R., Sakowicz R. Crystal structure of the mitotic spindle kinesin Eg5 reveals a novel conformation of the neck-linker. J. Biol. Chem. 276:2001;25496-25502
36. Wade R.H. Sequence landmark patterns identify and characterize protein families. Structure. 10:2002;1329-1336
37. Blangy A., Lane H.A., d'Herin P., Harper M., Kress M., Nigg E.A. Phosphorylation by p34cdc2 regulates spindle association of human Eg5, a kinesin-related motor essential for bipolar spindle formation in vivo. Cell. 83:1995;1159-1169
38. Mayer T.U., Kapoor T.M., Haggarty S.J., King R.W., Schreiber S.L., Mitchison T.J. Small molecule inhibitor of mitotic spindle bipolarity identified in a phenotype-based screen. Science. 286:1999;971-974
39. Bergens, G. et al. (2002). Mitotic kinesin-targeted antitumor agents: discovery, lead optimization and anti-tumor activity of a series of novel quinazolinones as inhibitors of kinesin spindle protein (KSP). Meeting abstracts, American Association for Cancer Research. 3648, AACR, San Francisco, CA.
40. Johnson, R.K. et al. (2002). SB-715992, a potent and selective inhibitor of the mitotic kinesin KSP, demonstrates broad-spectrum activity in advanced murine tumors and human tumor xenografts. Meeting abstracts, American Association for Cancer research. 1335, AACR, San Francisco, CA.
41. Miyamoto D.T., Perlman Z.E., Mitchison T.J., Shirasu-Hiza M. Dynamics of the mitotic spindle - potential therapeutic targets. Prog. Cell Cycle Res. 5:2003;349-360
42. Jablonski S.A., Liu S.T., Yen T.J. Targeting the kinetochore for mitosis-specific inhibitors. Cancer Biol. Ther. 2:2003;236
43. DeBonis S., Simorre J.P., Crevel I., Lebeau L., Skoufias D.A., Blangy A., et al. Interaction of the mitotic inhibitor monastrol with human kinesin Eg5. Biochemistry. 42:2003;338-349
44. Garcia-Saez I., Blot D., Kahn R., Kozielski F. Crystallization and preliminary crystallographic analysis of the motor domain of human kinetochore-associated protein CENP-E, using an automated crystallization procedure. Acta. Crystallog. sect. D, Biol. Crystallog. 60:2004;1158-1160
45. Otwinowsky Z., Minor W. Processing of X-ray diffraction data collected in oscillation mode. Methods Enzymol. 276:1997;307-326
46. Collaborative Computational Project, Number 4 (1994). The CCP4 Suite: programs for protein crystallography. Acta Crystallog. sect. D, Biol. Crystallog. 50:1994;760-763
47. Navaza J., Saludjian P. AMoRe: an automated molecular replacement program package. Methods Enzymol. 276:1997;581-594
48. Roussel, A. & Cambillau, C. (1991). The TURBO-FRODO graphics pachage. Silicon Graphics Geometry Partners Directory, vol. 81, Mountain View, CA.
49. Brünger A.T., Adams P.D., Clore G.M., DeLano W.L., Gros P., Grosse-Kunstleve R.W., et al. Crystallography and NMR system: a new software suite for macromolecular structure determination. Acta Crystallog. sect. D, Biol. Crystallog. 54:1998;905-921
50. Laskowski R., MacArthur M., Moss D., Thornton J. PROCHECK: a program to check the stereochemical quality of protein structures. J. Appl. Crystallog. 26:1993;91-97
51. Kraulis P.J. MOLSCRIPT: a program for producing both detailed and schematic plots of protein structures. J. Appl. Crysallog. 24:1991;946-950
52. Esnouf R.M. Further additions to MolScript version 1.4, including reading and contouring of electron-density maps. Acta Crystallog. sect. D, Biol. Crystallog. 55:1999;938-940
53. Gouet P., Courcell E., Stuart D.I., Metoz F. ESPript: multiple sequence alignments in PostScript. Bioinformatics. 15:1999;305-308
54. Kull F.J., Sablin E.P., Lau R., Fletterick R.J., Vale R.D. Crystal structure of the kinesin motor domain reveals a structural similarity to myosin. Nature. 380:1996;550-555