CENP-E is essential for reliable bioriented spindle attachment, but chromosome alignment can be achieved via redundant mechanisms in mammalian cells

Molecular Biology of the Cell

Volumn 12, Issue 9, 2001, Pages 2776-2789

  McEwen, B F ^a   Chan, G K T ^a   Zubrowski, B ^a   Savoian, M S ^a   Sauer, M T ^a   Yen, T J ^a  

^a WADSWORTH CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELL PROTEIN; CENTROMERE PROTEIN E; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CENTROMERE; CHROMOSOMAL LOCALIZATION; CHROMOSOME; CONTROLLED STUDY; MICROTUBULE; MITOSIS INHIBITION; MITOSIS SPINDLE; NONHUMAN; OSCILLATION; PRIORITY JOURNAL; PROTEIN DEPLETION; PROTEIN LOCALIZATION; SEQUENCE HOMOLOGY;

EID: 0035172929     PISSN: 10591524     EISSN: None     Source Type: Journal    
DOI: 10.1091/mbc.12.9.2776     Document Type: Article

References (42)

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4. 1 cells
5. Human Zw10 and ROD are mitotic checkpoint proteins that bind to kinetochores
6. Human BUBR1 is a mitotic checkpoint kinase that monitors CENP-E functions at the kinetochore and binds the cyclosome/APC
7. Characterization of the kinetochore binding domain of CENP-E reveals interactions with the kinetochore proteins CENP-F and hBUBR1
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13. The hBUB1 and hBUBR1 kinases sequentially assemble onto kinetochores during prophase with hBUBR1 concentrating at the kinetochore plates in mitosis
14. hBUB1 mediates assembly of hBUBR1, hMAD2, and hROD checkpoint proteins onto kinetochores
15. Kinetochores moving away from their associated pole do not exert a significant pushing force on the chromosome
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18. Antibodies to the kinesin motor domain and CENP-E inhibit microtubule depolymerization-dependent motion of chromosomes in vitro
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26. Correlative light and electron microscopy of mitotic cells in monolayer cultures
27. Oscillatory movements of monooriented chromosomes and their position relative to the spindle pole result from the ejection properties of the aster and half-spindle
28. Cleavage furrows formed between centrosomes lacking an intervening spindle and chromosomes contain microtubule Bundles, INCENP, and CHO1 but not CENP-E
29. The rate of poleward chromosome motion is attenuated in Drosaphila zw10 and rod mutants
30. CENP-E function at kinetochores is essential for chromosome alignment
31. Directional instability of kinetochore motility during chromosome congression and segregation in mitotic newt lung cells: A push-pull mechanism
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40. CENP-E is a putative kinetochore motor that accumulates just before mitosis
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42. Kinetochore microtubule dynamics and the metaphase-anaphase transition