The kinesin-like protein CENP-E is kinetochore-associated throughout poleward chromosome segregation during anaphase-A

Journal of Cell Science

Volumn 109, Issue 5, 1996, Pages 961-969

  Brown, Kevin D ^a,c   Wood, Kenneth W ^a,b   Cleveland, Don W ^a,b  

^a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c NATIONAL INSTITUTES OF HEALTH   (United States)

Author keywords

Anaphase A; Anaphase B; CENP E; Kinetochore; Microtubule motor; Mitosis

Indexed keywords

ANTIBODY; KINESIN;

ANAPHASE; ANIMAL CELL; ARTICLE; CENTROMERE; CHROMOSOME SEGREGATION; HUMAN; HUMAN CELL; MICROTUBULE; MITOSIS SPINDLE; NONHUMAN; PRIORITY JOURNAL; PROTEIN LOCALIZATION;

ANAPHASE; ANIMALS; CELL POLARITY; CHROMOSOMAL PROTEINS, NON-HISTONE; FEMALE; HUMANS; KINESIN; KINETOCHORES; MALE; MITOSIS; MUNTJACS; VIDEO RECORDING;

ANIMALIA; MUNTIACUS MUNTJAK;

EID: 0029903059     PISSN: 00219533     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (36)

1. Balczon, R. B., Bao, L. and Zimmer, W. E. (1994). PCM-1, a 228-kD centrosome autoantigen with a distinct cell cycle distribution. J. Cell Biol. 124, 783-793.
2. Brinkley, B. R., Valdivia, M. M., Tousson, A. and Brenner, S. R. (1984). Compound kinetochores of the Indian muntjac. Evolution by linear fusion of unit kinetochores. Chromosoma 91, 1-11.
3. Brown, K. D., Coulson, R. M. R., Yen, T. J. and Cleveland, D. W. (1994). Cyclin-like accumulation and loss of the putative kinetochore motor CENP-E results from coupling continuous synthesis with specific degradation at the end of mitosis. J. Cell Biol. 125, 1303-1312.
4. Cooke, C. A., Bernat, R. L. and Earnshaw, W. C. (1990). CENP-B: a major human centromere protein located beneath the kinetochore. J. Cell Biol. 110, 1475-1488.
5. Endow, S. A., Kang, S. J., Satterwhite, L. L., Rose, M. D., Skeen, V. P. and Salmon, E. D. (1994). The yeast Kar3 is a minus-end microtubule motor protein that destabilizes microtubules preferentially at the minus ends. EMBO J. 13, 2708-2713.
6. Euteneur, U. and McIntosh, J. R. (1981). Structural polarity of kinetochore microtubules in PtK1 cells. J. Cell Biol. 89, 338-345.
7. Fan, H. and Penman, S. (1970). Regulation of protein synthesis in mammalian cells. J. Mol. Biol. 50, 655-670.
8. Goldstein, L. S. B. (1993). With apologies to Scheherazade: tails of 1001 kinesin motors. Annu. Rev. Genetics. 27, 319-351.
9. Gorbsky, G. J., Sammak, P. J. and Borisy, G. G. (1987). Chromosomes move poleward in anaphase along stationary microtubules that coordinately disassemble from their kinetochore ends. J. Cell Biol. 104, 9-18.
10. Harlow, E. and Lane, D. (1988). Antibodies: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
11. Hayden, J. H., Bowser, S. S. and Rieder, C. L. (1990). Kinetochores capture astral microtubules during chromosome attachment to the mitotic spindle: direct visualization in live newt cells. J. Cell Biol. 111, 1039-1045.
12. Hyman, A. A. and Mitchison, T. J. (1991). Two different microtubule-based motor activities with opposite polarities in kinetochores. Nature 351, 206-211.
13. Koshland, D., Mitchison, T. J. and Kirschner, M. W. (1988). Polewards chromosome movement driven by microtubule depolymerization in vitro. Nature 331, 499-501.
14. Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the bacteriophage T4. Nature 337, 650-655.
15. Li, Y. Y., Yeh, E., Hays, T. and Bloom, K. (1993). Disruption of mitotic spindle orientation in a yeast dynein mutant. Proc. Nat. Acad. Sci. USA 90, 10096-10100.
16. Liao, H., Li, G. and Yen, T. J. (1994). Mitotic regulation of microtubule cross-linking activity of CENP-E kinetochore protein. Science 265, 394-398.
17. Lombillo, V. A., Nislow, C., Yen, T. J., Gelfand, V. I. and McIntosh, J. R. (1995). Antibodies to the kinesin motor domain and CENP-E inhibit microtubule depolymerization-dependent motion of chromosomes in vitro. J. Cell Biol. 128, 107-115.
18. McDonald, H. B., Stewart, R. J. and Goldstein, L. S. B. (1990) The kinesin like ncd protein is a minus end directed microtubule motor. Cell 63, 1159-1165.
19. McIntosh, J. R. (1985). Spindle structure and the mechanisms of chromosome movement. In Aneuploidy: Etiology and Mechanisms (ed. V. Dellarco, P. E. Voytek and A. Hollaender), pp. 197-229. Plenum Publishing, New York.
20. Mitchison, T. J. and Kirschner, M. W. (1985a). Properties of the kinetochore in vitro. 1. Microtubule nucleation and tubulin binding. J. Cell Biol. 101, 755-765.
21. Mitchison, T. J. and Kirschner, M. W. (1985b). Properties of the kinetochore in vitro. 2. Microtubule capture and ATP translocation. J. Cell Biol. 101, 767-777.
22. Mitchison, T. J. and Salmon, E. D. (1992). Poleward kinetochore fiber movement occurs during both metaphase and anaphase-A in newt lung cell mitosis. J. Cell Biol. 119, 569-582.
23. Nicklas, R. B. (1989). The motor for poleward chromosome movement in anaphase is in or near the kinetochore. J. Cell Biol. 109, 2245-2255.
24. Pfarr, C. M., Coue, M., Grissom, P. M., Hays, T. S., Porter, M. E. and McIntosh, J. R. (1990). Cytoplasmic dynein is localized to the kinetochores during mitosis. Nature 345, 263-265.
25. Rieder, C. L. and Alexander, S. P. (1990). Kinetochores are transported poleward along a single astral microtubule during chromosome attachment to the spindle in newt lung cells. J. Cell Biol. 110, 81-95.
26. Saunders, W. S., Koshland, D., Eshel, D., Gibbons, I. R. and Hoyt, M. A. (1995). Saccharomyces cerevisiae kinesin- and dynein-related proteins required for anaphase chromosome segregation. J. Cell Biol. 128, 617-624.
27. Smith, P. K., Krhon, P. I., Hermanson, G. T., Malia, A. K., Gartner, F. H., Provenzaus, M. D., Fujimoto, E. K., Goeke, N. M., Olson, B. J. and Klenk, D. C. (1985). Measurement of protein using bicinchonic acid. Anal. Biochem. 150, 76-85.
28. Steuer, E. R., Wordeman, L., Schroer, T. A. and Sheetz, M. P. (1990). Localization of cytoplasmic dynein to mitotic spindles and kinetochores. Nature 345, 266-268.
29. Studier, F. W., Rosenberg, A. H., Dunn, J. J. and Dubendorff, J. W. (1990). Use of T7 RNA polymerase to direct expression of cloned genes. Meth. Enzymol. 185, 60-89.
30. Thrower, D. A., Jordan, M. A., Schaar, B., Yen, T. J. and Wilson, L. (1995). Mitotic HeLa cells contain a CENP-E-associated minus end-directed microtubule motor. EMBO J. 14, 918-926.
31. Towbin, H., Staehelin, T. and Gordon, J. (1979). Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Nat. Acad. Sci. USA 76, 4350-4354.
32. Vaisberg, E. A., Koonce, M. P. and McIntosh, J. R. (1993). Cytoplasmic dynein plays a role in mammalian mitotic spindle formation. J. Cell Biol. 123, 849-858.
33. Walker, R. A., Salmon, E. D. and Endow, S. A. (1990). The Drosophila claret segregation protein is a minus-end directed motor molecule. Nature 347, 780-782.
34. Yen, T. J., Compton, D. A., Wise, D., Zinkowski, R. P., Brinkley, B. R., Earnshaw, W. C. and Cleveland, D. W. (1991). CENP-E, a human centromere associated protein required for progression from metaphase to anaphase. EMBO J. 10, 1245-1254.
35. Yen, T. J., Li, G., Schaar, B. T., Szilak, I. and Cleveland, D. W. (1992). CENP-E is a putative kinetochore motor that accumulates just before mitosis. Nature 359, 536-539.
36. Zinkowski, R. P., Meyne, J. and Brinkley, B. R. (1991). The centromere-kinetochore complex: a repeat subunit model. J. Cell Biol. 113, 1091-1110.