The effect of a single nucleotide substitution in the splicing silencer in the tat/rev intron on HIV type 1 envelope expression

AIDS Research and Human Retroviruses

Volumn 22, Issue 1, 2006, Pages 76-82

  Paca Uccaralertkun, Saowakon ^a   Damgaard, Christian Kroun ^b   Auewarakul, Prasert ^c   Thitithanyanont, Arunee ^a   Suphaphiphat, Pirada ^d   Essex, Max ^d   Kjems, Jørgen ^b   Lee, Tun Hou ^d  

^a Mahidol University   (Thailand)

^b AARHUS UNIVERSITY   (Denmark)

^c MAHIDOL UNIVERSITY   (Thailand)

^d HARVARD SCHOOL OF PUBLIC HEALTH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BETA GALACTOSIDASE; HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN; MESSENGER RNA; REV PROTEIN; TRANSACTIVATOR PROTEIN; VIRUS ENVELOPE PROTEIN;

ANIMAL CELL; ARTICLE; CELL STRAIN COS7; CONTROLLED STUDY; ENVELOPE GENE; EXON; EXPRESSION VECTOR; GEL MOBILITY SHIFT ASSAY; GENE EXPRESSION; GENETIC TRANSFECTION; HUMAN IMMUNODEFICIENCY VIRUS 1; INTRON; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROVIRUS; RECOMBINANT PLASMID; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA SPLICING; SILENCER ELEMENT; VIRUS MUTATION; WESTERN BLOTTING;

ANIMALS; BASE SEQUENCE; CERCOPITHECUS AETHIOPS; COS CELLS; GENE EXPRESSION; GENE PRODUCTS, REV; GENE SILENCING; GENES, TAT; HIV-1; INTRONS; POINT MUTATION; PROTEIN BINDING; RNA SPLICING; RNA, MESSENGER; RNA, VIRAL;

EID: 32144450808     PISSN: 08892229     EISSN: None     Source Type: Journal    
DOI: 10.1089/aid.2006.22.76     Document Type: Article

References (39)

1. Muesing MA, Smith DH, Cabradilla CD, Benton CV, Lasky LA, and Capon DJ: Nucleic acid structure and expression of the human AIDS/lymphadenopathy retrovirus. Nature 1985;313:450-458.
2. Schwartz S, Felber BK, Benko DM, Fenyo E, and Pavlakis GN: Cloning and functional analysis of multiply spliced mRNA species of human immunodeficiency virus type I. J Virol 1990;64: 2519-2529.
3. Staffa A and Cochrane A: The tat/rev intron of human immunodeficiency virus type 1 is inefficiently spliced because of suboptimal signals in the 3′ splice site. J Virol 1994;68:3071-3079.
4. Dyhr-Mikkelsen H and Kjems J: Inefficient spliceosome assembly and abnormal branch site selection in splicing of an HIV-1 transcript in vitro. J Biol Chem 1995;270:24060-24066.
5. O'Reilly MM, McNally MT, and Beemon KL: Two strong 5′ splice sites and competing, suboptimal 3′ splice sites involved in alternative splicing of human immunodeficiency virus type 1 RNA. Virology 1995;213:373-385.
6. Jacquenet S, Ropers D, Bilodeau PS, Damier L, Mougin A, Stoltzfus CM, and Branlant C: Conserved stem-loop structures in the HIV-1 RNA region containing the A3 3′ splice site and its cis-regulatory element: Possible involvement in RNA splicing. Nucleic Acids Res 2001;29:464-478.
7. Tange TØ and Kjems J: SF2/ASF binds to a splicing enhancer in the third HIV-1 tat exon and stimulates U2AF binding independently of the RS domain. J Mol Biol 2001;312:649-662.
8. Staffa A and Cochrane A: Identification of positive and negative splicing regulatory elements within the terminal tat-rev exon of human immunodeficiency virus type 1. Mol Cell Biol 1995;15: 4597-4605.
9. Amendt BA, Si ZH, and Stoltzfus CM: Presence of exon splicing silencers within human immunodeficiency virus type 1 tat exon 2 and tat-rev exon 3: Evidence for inhibition mediated by cellular factors. Mol Cell Biol 1995;15(8):4606-4615.
10. Mayeda A, Screaton GR, Chandler SD, Fu XD, and Krainer AR: Substrate specificities of SR proteins in constitutive splicing are determined by their RNA recognition motifs and composite pre-mRNA exonic elements. Mol Cell Biol 1999;19(3):1853-1863.
11. Tacke R, Tohyama M, Ogawa S, and Manley J: Human Tra2 proteins are sequence-specific activators of pre-mRNA splicing. Cell 1998;93(1):139-148.
12. Liu H, Zhang M, and Krainer AR: Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins. Genes Dev 1998;12(13):1998-2012.
13. Kamrnler S, Leurs C, Freund M, Krummheuer J, Seidel K, Tange TØ, Lund MK, Kjems J, Scheid A, and Schaal H: The sequence complementarity between HIV-1 5′ splice site SD4 and U1 snRNA determines the steady-state level of an unstable env pre-mRNA. RNA 2001;7:421-434.
14. Tacke R and Manley J: Determinants of SR protein specificity. Curr Opin Cell Biol 1999;11(3):358-362.
15. Blencowe BJ, Bowman JA, McCracken S, and Rosonina E: SR-related proteins and the processing of messenger RNA precursors. Biochem Cell Biol 1999;77(4):277-291.
16. Blanchette M and Chabot B: Modulation of exon skipping by high-affinity hnRNP A1-binding sites and by intron elements that repress splice site utilization. EMBO J 1999;18:1939-1952.
17. Chabot B, Blanchette M, Lapierre I, and La Branche H: An intron element modulating 5′ splice site selection in the hnRNP A1 pre mRNA interacts with hnRNP A1. Mol Cell Biol 1997;17: 1776-1786.
18. Eperon IC, Makarova OV, Mayeda A, Munroe SH, Caceres JF, Hayward DG, and Krainer AR: Selection of alternative 5′ splice sites: Role of U1 snRNP and models for the antagonistic effects of SF2/ASF and hnRNP A1. Mol Cell Biol 2000;20:8303-8318.
19. Gorlach M, Burd CG, Portman DS, and Dreyfuss G: The hnRNP proteins. Mol Biol Rep 1993;18:73-78.
20. Caputi M and Zahler A: SR proteins and hnRNP H regulate the splicing of the HIV-1 tev-specific exon 6D. EMBO J 2002; 21(4):845-855.
21. Del Gatto-Konczak F, Olive M, Gesnel MC, and Breathnach R: hnRNP A1 recruited to an exon in vivo can function as an exon splicing silencer. Mol Cell Biol 1999;19:251-260.
22. Tange TØ, Damgaard CK, Guth S, Valcarcel J, and Kjems J: The hnRNP A1 protein regulates HIV-1 tat splicing via a novel intron silencer element. EMBO J 2001;20:5748-5758.
23. Si ZH, Rauch D, and Stoltzfus CM: The exon splicing silencer in human immunodeficiency virus type 1 Tat exon 3 is bipartite and acts early in spliceosome assembly. Mol Cell Biol 1998;18:5404-5413.
24. Caputi M, Mayeda A, Krainer AR, and Zahler AM: hnRNP A/B proteins are required for inhibition of HIV-1 pre-mRNA splicing. EMBO J 1999;18:4060-4067.
25. Zhu J, Mayeda A, and Krainer AR: Exon identity established through differential antagonism between exonic splicing silencer-bound hnRNP A1 and enhancer-bound SR proteins. Mol Cell 2001;8(6):1351-1361.
26. Damgaard CK, Tange TØ, and Kjems J: hnRNP A1 controls HIV-1 mRNA splicing through cooperative binding to intron and exon splicing silencers in the context of a conserved secondary structure. RNA 2002;8:1401-1415.
27. Marchand V, Mereau A, Jacquenet S, Thomas D, Mougin A, Gattoni R, Stevenin J, and Branlant C: A janus splicing regulatory element modulates HIV-1 tat and rev mRNA production by coordination of hnRNP A1 cooperative binding. J Mol Biol 2002; 323:629-652.
28. Suptawiwat O, Sutthent S, Lee T, and Auewarakul P: Intragenic HIV-1 env sequences that enhance Gag expression. Virology 2003;309:1-9.
29. Hoffman TL and Doms RW: HIV-1 envelope determinants for cell tropism and chemokine receptor use. Mol Membr Biol 1999; 16:57-65.
30. Berger EA: HIV entry and tropism: The chemokine receptor connection. AIDS 1997;11(Suppl. A):S3-S16.
31. Wei X, Decker JM, Wang S, Hui H, Kappes JC, Wu X, Salazar-Gonzalez JF, Salazar MG, Kilby JM, Saag MS, Komarova NL, Nowak MA, Hahn BH, Kwong PD, and Shaw GM: Antibody neutralization and escape by HIV-1. Nature 2003;422:307-312.
32. Bjorndal A, Deng H, Jansson M, Fiore JR, Colognesi C, Karlsson A, Albert J, Scarlatti G, Littman DR, and Fenyo EM: Coreceptor usage of primary human immunodeficiency virus type 1 isolates varies according to biological phenotype. J Virol 1997;71:7478-7487.
33. Hope TJ: The ins and outs of HIV Rev Arch Biochem Biophys 1999;365(2):186-191.
34. Pollard VW and Malim MH: The HIV-1 Rev protein. Annu Rev Microbiol 1998;52:491-532.
35. Hammarskjold ML, Heimer J, Hammarskjold B, Sangwan I, Albert L, and Rekosh D: Regulation of human immunodeficiency virus env expression by the rev gene product. J Virol 1989; 63:1959-1966.
36. Pongoski J, Asai K, and Cochrane A: Positive and negative modulation of human immunodeficiency virus type 1 Rev function by cis and trans regulators of viral RNA splicing. J Virol 2002;76 (10):5108-5120.
37. Purcell D and Martin MA: Alternative splicing of human immunodeficiency virus type 1 mRNA modulates viral protein expression, replication, and infectivity. J Virol 1993;67:6365-6378.
38. Cleghorn FR, Jack N, Carr JK, Edwards J, Mahabir B, Sill A, McDanal CB, Connolly SM, Goodman D, Bennetts RQ, O'Brien TR, Weinhold KJ, Bartholomew C, Blattner WA, and Greenberg ML: A distinctive clade B HIV type 1 is heterosexually transmitted in Trinidad and Tobago. Proc Natl Acad Sci USA 2000;97: 10532-10537.
39. Carr JK: Diverse BF recombinants have spread widely since the introduction of HIV-1 into South America. AIDS 2001;15:F41-47.