Selection of alternative 5' splice sites: Role of U1 snRNP and models for the antagonistic effects of SF2/ASF and hnRNP A1

Molecular and Cellular Biology

Volumn 20, Issue 22, 2000, Pages 8303-8318

  Eperon, I C ^a   Makarova, O V ^a   Mayeda, A ^a   Munroe, S H ^a   Caceres, J F ^a   Hayward, D G ^a   Krainer, A R ^a  

^a UNIVERSITY OF LEICESTER   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

MUTANT PROTEIN; SMALL NUCLEAR RIBONUCLEOPROTEIN;

ALTERNATIVE RNA SPLICING; ARTICLE; COMPETITIVE INHIBITION; CROSS LINKING; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN RNA BINDING;

ALTERNATIVE SPLICING; BINDING SITES; BINDING, COMPETITIVE; HETEROGENEOUS-NUCLEAR RIBONUCLEOPROTEIN GROUP A-B; HETEROGENEOUS-NUCLEAR RIBONUCLEOPROTEINS; MODELS, BIOLOGICAL; NUCLEAR PROTEINS; RIBONUCLEOPROTEIN, U1 SMALL NUCLEAR; RIBONUCLEOPROTEINS; RNA PRECURSORS; RNA SPLICE SITES; RNA, MESSENGER; RNA-BINDING PROTEINS;

EID: 0033762534     PISSN: 02707306     EISSN: None     Source Type: Journal    
DOI: 10.1128/MCB.20.22.8303-8318.2000     Document Type: Article

References (97)

1. Origins of binding specificity of the A1 heterogeneous nuclear ribonucleoprotein
2. hnRNP A1 binds promiscuously to oligoribonucleotides: Utilization of random and homo-oligonucleotides to discriminate sequence from base-specific binding
3. Cross-intron bridging interactions in the yeast commitment complex are conserved in mammals
4. Purification of small nuclear ribonucleoprotein particles with antibodies against modified nucleosides of small nuclear RNAs
5. A sensitive procedure for mapping the boundaries of RNA elements binding in vitro translated proteins defines a minimal hepatitis B virus encapsidation signal
6. Differential binding of heterogeneous nuclear ribonucleoproteins to mRNA precursors prior to spliceosome assembly in vitro
7. Analysis of the RNA-recognition motif and RS and RGG domains: Conservation in metazoan pre-mRNA splicing factors
8. Activation of c-src neuron-specific splicing by an unusual RNA element in vivo and in vitro
9. A highly stable duplex structure sequesters the 5' splice site region of hnRNP A1 alternative exon 7B
10. Modulation of exon skipping by high-affinity hnRNP Al-binding sites and by intron elements that repress splice site utilization
11. Identification of a bidirectional splicing enhancer: Differential involvement of SR proteins in 5' or 3' splice site activation
12. RNA binding specificity of hnRNP A1: Significance of hnRNP A1 high-affinity binding sites in pre-mRNA splicing
13. Interaction of hnRNP A1 with snRNPs and pre-mRNAs: Evidence for a possible role of A1 RNA annealing activity in the first steps of spliceosome assembly
14. Functional analysis of pre-mRNA splicing factor SF2/ASF structural domains
15. Mammalian pre-mRNA splicing factors
16. Role of the modular domains of SR proteins in subnuclear localization and alternative splicing specificity
17. Regulation of alternative splicing in vivo by overexpression of antagonistic splicing factors
18. Both phosphorylation and dephosphorylation of ASF/SF2 are required for pre-mRNA splicing in vitro
19. hnRNP A/B proteins are required for inhibition of HIV-1 pre-mRNA splicing
20. hnRNP A1 selectively interacts through its Gly-rich domain with different RNA-binding proteins
21. Mammalian heterogeneous ribonucleoprotein A1 and its constituent domains: Nucleic acid interaction, structural stability and self-association
22. Characterization and cloning of the human splicing factor 9G8: A novel 35 kDa factor of the serine/arginine protein family
23. An intron element modulating 5' splice site selection in the hnRNP A1 pre-mRNA interacts with hnRNP A1
24. Recognition of mutant and cryptic 5' splice sites by the U1 small nuclear ribonucleoprotein in vitro
25. Identification of proteins that interact with exon sequences, splice sites, and the branchpoint sequence during each stage of spliceosome assembly
26. Mammalian heterogeneous nuclear ribonucleoprotein complex protein A1: Large-scale overproduction in Escherichia coli and cooperative binding to single-stranded nucleic acids
27. Mutations in the conserved loop of human U5 snRNA generate use of novel cryptic 5' splice sites in vivo
28. Influences of separation and adjacent sequences on the use of alternative 5' splice sites
29. hnRNP A1 recruited to an exon in vivo can function as an exon splicing silencer
30. hnRNP proteins and the biogenesis of mRNA
31. A short sequence within two purine-rich enhancers determines 5' splice site specificity
32. Pathways for selection of 5' splice sites by U1 snRNPs and SF2/ASF
33. Effects of RNA secondary structure on alternative splicing of pre-mRNA: Is folding limited to a region behind the transcribing RNA polymerase?
34. Luc7p, a novel yeast U1 snRNP protein with a role in 5' splice site recognition
35. Specific commitment of different pre-mRNAs to splicing by single SR proteins
36. General splicing factors SF2 and SC35 have equivalent activities in vitro, and both affect alternative 5' and 3' splice site selection
37. A protein factor, ASF, controls cell-specific alternative splicing of SV40 early pre-mRNA in vitro
38. Regulated tissue-specific expression of antagonistic pre-mRNA splicing factors
39. U1-snRNA promotes the selection of nearby 5' splice sites by U6 snRNA in mammalian cells
40. A role for the M9 transport signal of hnRNP A1 in mRNA nuclear export
41. U1 snRNP-ASF/SF2 interaction and 5' splice site recognition - Characterization of required elements
42. Protein-protein interactions and 5'-splice-site recognition in mammalian mRNA precursors
43. The essential pre-mRNA splicing factor SF2 influences 5' splice site selection by activating proximal sites
44. Functional expression of cloned human splicing factor SF2: Homology to RNA-binding proteins, U1 70K, and Drosophila splicing regulators
45. A splicing enhancer in the human fibronectin alternate ED1 exon interacts with SR proteins and stimulates U2 snRNP binding
46. Hierarchy for 5' splice site preference determined in vivo
47. Early commitment of yeast pre-mRNA to the spliceosome pathway
48. A nuclear cap-binding complex facilitates association of U1-snRNP with the cap-proximal 5' splice site
49. Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins
50. Assembly of specific SR protein complexes on distinct regulatory elements of the Drosophila doublesex splicing enhancer
51. Synergistic interactions between 2 distinct elements of a regulated splicing enhancer
52. SR proteins and splicing control
53. Modulation of exon skipping and inclusion by heterogeneous nuclear ribonucleoprotein A1 and pre-mRNA splicing factor SF2/ASF
54. Regulation of alternative pre-mRNA splicing by hnRNP A1 and splicing factor SF2
55. Function of conserved domains of hnRNP A1 and other hnRNP A/B proteins
56. Distinct functions of the closely related tandem RNA-recognition motifs of hnRNP A1
57. Beta-globin transcripts carrying a single intron with three adjacent nucleotides of 5' exon are efficiently spliced in vitro irrespective of intron position or surrounding exon sequences
58. Short donor site sequences inserted within the intron of β-globin pre-mRNA serve for splicing in vitro
59. Substrate specificities of SR proteins in constitutive splicing are determined by their RNA recognition motifs and composite pre-mRNA exonic elements
60. An ATP-independent complex commits pre-mRNA to the mammalian spliceosome assembly pathway
61. A functional association between the 5' and 3' splice sites is established in the earliest prespliceosome complex (E) in mammals
62. The dynamics of a pre-mRNA splicing factor in living cells
63. Heterogeneous nuclear ribonucleo-protein A1 catalyzes RNA·RNA annealing
64. Flipping the switch to an active spliceosome
65. Mechanism for cryptic splice site activation during pre-mRNA splicing
66. Splice site selection and ribonucleoprotein complex assembly during in vitro pre-messenger RNA splicing
67. Mutations in yeast U5 snRNA alter the specificity of 5' splice-site cleavage
68. The pre-mRNA 5' cap determines whether U6 small nuclear RNA succeeds U1 small nuclear ribonucleoprotein particle at 5' splice sites
69. hnRNP proteins: Localization and transport between the nucleus and the cytoplasm
70. Rapid assembly and disassembly of complementary DNA strands through an equilibrium intermediate state mediated by A1 hnRNP protein
71. Interaction of the U1 snRNP with nonconserved intronic sequences affects 5' splice site selection
72. A subset of SR proteins activates splicing of the cardiac troponin T alternative exon by direct interactions with an exonic enhancer
73. A role for exon sequences and splice-site proximity in splice-site selection
74. Multiple distinct splicing enhancers in the protein-coding sequences of a constitutively spliced pre-mRNA
75. Selection and characterization of pre-mRNA splicing enhancers: Identification of novel SR protein-specific enhancer sequences
76. Identification and characterization of 3 members of the human SR family of pre-mRNA splicing factors
77. 3' Splice site recognition in S. cerevisiae does not require base pairing with U1 snRNA
78. Identification of functional U1 snRNA-pre-mRNA complexes committed to spliceosome assembly and splicing
79. Exon skipping by overexpression of a Drosophila heterogeneous nuclear ribonucleoprotein in vivo
80. Reduction of enzyme adsorption to polypropylene surfaces in the presence of a nonionic detergent
81. Nuclear RNA export
82. General splicing factor SF2/ASF promotes alternative splicing by binding to an exonic splicing enhancer
83. Modulation of 5' splice site choice in pre-messenger RNA by 2 distinct steps
84. A splicing enhancer complex controls alternative splicing of doublesex pre-mRNA
85. A splicing enhancer exhibits both constitutive and regulated activities
86. The SR protein family: Pleiotropic functions in pre-mRNA splicing
87. Overexpression of the SR proteins ASF/SF2 and SC35 influences alternative splicing in vivo in diverse ways
88. Genetic analysis of the SR protein ASF/SF2: Interchangeability of RS domains and negative control of splicing
89. Phosphorylation of the ASF/SF2 RS domain affects both protein-protein and protein-RNA interactions and is necessary for splicing
90. The A1 and A1(B) proteins of heterogeneous nuclear ribonucleoparticles modulate 5' splice site selection in vivo
91. Distinct functions of SR proteins in alternative pre-mRNA splicing
92. Distinct functions of SR proteins in recruitment of U1 small nuclear ribonucleoprotein to alternative 5' splice sites
93. Identification of eight proteins that cross-link to pre-mRNA in the yeast commitment complex
94. Functional properties of p54, a novel SR protein active in constitutive and alternative splicing
95. A compensatory base change in U1 snRNA suppresses a 5' splice site mutation
96. Functional domains of the human splicing factor ASF/SF2
97. The human splicing factor ASF/SF2 can specifically recognize pre-mRNA 5' splice sites