The exon splicing silencer in human immunodeficiency virus type 1 tat exon 3 is bipartite and acts early in spliceosome assembly

Molecular and Cellular Biology

Volumn 18, Issue 9, 1998, Pages 5404-5413

  Si, Zhi Hai ^a   Rauch, Dan ^a   Martin Stoltzfus, C ^a  

^a UNIVERSITY OF IOWA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

TRANSACTIVATOR PROTEIN; VIRUS RNA;

ARTICLE; GENE EXPRESSION REGULATION; HUMAN IMMUNODEFICIENCY VIRUS 1; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; RNA SPLICING; RNA STRUCTURE; RNA TRANSLATION; SPLICEOSOME; TRANSLATION REGULATION;

HUMAN IMMUNODEFICIENCY VIRUS; HUMAN IMMUNODEFICIENCY VIRUS 1; RNA VIRUSES;

EID: 0031841948     PISSN: 02707306     EISSN: None     Source Type: Journal    
DOI: 10.1128/MCB.18.9.5404     Document Type: Article

References (52)

1. Adams, M. D., R. S. Tarng, and D. C. Rio. 1997. The alternative splicing factor PSI regulates P-element third intron splicing in vivo. Genes Dev. 11:129-138.
2. Amendt, B. A., D. Hesslein, L.-J. Chang, and C. M. Stoltzfus. 1994. Presence of negative and positive cis-acting RNA splicing elements within and flanking the first tat coding exon of human immunodeficiency virus type 1. Mol. Cell. Biol. 14:3960-3970.
3. Amendt, B. A., Z.-H. Si, and C. M. Stoltzfus. 1995. Presence of exon splicing silencers within human immunodeficiency virus type 1 tat exon 2 and tat-rev exon 3: evidence for inhibition mediated by cellular factors. Mol. Cell. Biol. 15:4606-4615.
4. Arrigo, S., and K. Beemon. 1988. Regulation of Rous sarcoma virus RNA splicing and stability. Mol. Cell. Biol. 8:4858-4867.
5. Bennett, M., M. S. Michaud, J. Kingston, and R. Reed. 1992. Protein components specifically associated with pre-spliceosome and spliceosome com plexes. Genes Dev. 6:1986-2000.
6. Bennett, M., S. Piñol-Roma, D. Staknis. G. Dreyfuss, and R. Reed. 1992. Differential binding of heterogeneous nuclear ribonucleoproteins to mRNA precursors prior to spliceosome assembly in vitro. Mol. Cell. Biol. 12:3165-3175.
7. Bouck, J., X.-D. Fu, A. M. Skalka, and R. A. Katz. 1996. Genetic selection for balanced retroviral splicing: novel regulation involving the second step can be mediated by transitions in the polypyrimidine tract. Mol. Cell. Biol. 15:2663-2671.
8. Caputi, M., G. Casari, S. Guenzi, R. Tagliabue, A. Sidoli, C. A. Melo, and F. E. Baralle. 1994. A novel bipartite splicing enhancer modulates the differential processing of the human fibronectin EDA exon. Nucleic Acids Res. 22:1018-1022.
9. Champion-Arnaud, P., O. Gozani, L. Palandjian, and R. Reed. 1995. Accumulation of a novel spliceosomal complex on pre-mRNAs containing branch I site mutations. Mol. Cell. Biol. 15:5750-5756.
10. Chiara, M. D., O. Gozani, M. Bennett, P. Champion-Arnaud, L. Palandjian, and R. Reed. 1996. Identification of proteins that interact with exon se- quences, splice sites, and the branchpoint sequence during each stage of spliceosome assembly. Mol. Cell. Biol. 16:3317-3326.
11. Coffin, J. M. 1990. Retroviridae and their replication, p. 1437-1500. In B. N. Fields and D. M. Knipe (ed.), Fields virology. Raven Press, New York, N.Y.
12. Cullen, B. R. 1991. Regulation of human immunodeficiency virus replication. Annu. Rev. Microbiol. 45:219-250.
13. Damier, L., L. Domenjoud, and C. Branlant. 1997. The D1-A2 and D2-A2 pairs of splice sites from HIV-1 are highly efficient in vitro, in spite of an unusual branch site. Biochem. Biophys. Res. Commun. 237:182-187.
14. Del Gatto, F., and R. Breathnach. 1995. Exon and intron sequences, respectively, repress and activate splicing of a fibroblast growth factor receptor 2 alternative exon. Mol. Cell. Biol. 15:4825-4834.
15. Del Gatto, F., M.-C. Gesnel, and R. Breathnach. 1996. The exon sequence TAGG can inhibit splicing. Nucleic Acids Res. 24:2017-2021.
16. Dignam, J. D., R. M. Lebovitz, and R. G. Roeder. 1983. Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei. Nucleic Acids Res. 11:1475-1489.
17. Dyhr-Mikkelsen, H., and J. Kjems. 1995. Inefficient spliceosome assembly and abnormal branch site selection in splicing of an HIV-1 transcript in vitro. J. Biol. Chem. 270:24060-24066.
18. Felber, B. K., C. M. Drysdale, and G. N. Pavlakis. 1990. Feedback regulation of human immunodeficiency virus type 1 expression by the Rev protein. J. Virol. 64:3734-3741.
19. Fischer, U., S. Meyer, M. Teufel, C. Heckel, R. Luhrmann, and G. Rautmann. 1994. Evidence that HIV-1 Rev directly promotes the nuclear export of unspliced RNA. EMBO J. 13:4105-4112.
20. Fu, X.-D., R. A. Katz, A. M. Skalka, and T. Maniatis. 1991. The role of branchpoint and 3′-exon sequences in the control of balanced splicing of avian retrovirus RNA. Genes Dev. 5:211-220.
21. Guatelli, J. C., T. R. Gingeras, and D. D. Richman. 1990. Alternative splice acceptor utilization during human immunodeficiency virus type 1 infection of cultured cells. J. Virol. 64:4093-4098.
22. Katz, R. A., and A. M. Skalka. 1990. Control of retroviral RNA splicing through maintenance of suboptimal processing signals. Mol. Cell. Biol. 10: 696-704.
23. Konarska, M. M. 1989. Analysis of splicing complexes and small ribonucleoprotein particles by native gel electrophoresis. Methods Enzymol. 180:442-453.
24. Konarska, M. M., and P. A. Sharp. 1986. Electrophoretic separation of complexes involved in the splicing of precursors to mRNA. Cell 46:845-855.
25. Krainer, A. R., T. Maniatis, B. Ruskin, and M. R. Green. 1984. Normal and mutant human beta-globin pre-mRNAs are faithfully and efficiently spliced in vitro. Cell 36:993-1005.
26. Kramer, A. 1996. The structure and function of proteins involved in mammalian pre-mRNA splicing. Annu. Rev. Biochem. 65:367-409.
27. Liaw, G. 1994. Improved protocol for directional multimerization of a DNA fragment. BioTechniques 17:668-670.
28. Luciw, P. A. 1996. Human immunodeficiency viruses and their replication, p. 1881-1952. In B. N. Fields, D. M. Knipe, and P. M. Howley (ed.). Fields virology. Lippincott-Raven Publishers, Philadephia, Pa.
29. Michaud, S., and R. Reed. 1991. An ATP-independent complex commits pre-mRNA to the mammalian spliceosome assembly pathway. Genes Dev. 5:2534-2546.
30. Michaud, S., and R. Reed. 1993. A functional association between the 5' and 3' splice site is established in the earliest prespliceosome (E) complex in mammals. Genes Dev. 7:1008-10020.
31. Moore, M. J., C. C. Query, and P. A. Sharp. 1993. Splicing of precursors to mRNAs by the spliceosome, p. 303-357. In R. F. Gesteland and J. R. Atkins (ed.), The RNA world. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
32. Muesing, M. A., D. H. Smith, C. D. Cabradilla, C. V. Benton, L. A. Lasky, and D. J. Capon. 1985. Nucleic acid structure and expression of the human AIDS/lymphadenopathy retrovirus. Nature 313:450-458.
33. O'Reilly, M. M., M. T. McNally, and K. L. Beemon. 1995. Two strong 5′ splice sites and competing suboptimal 3′ splice sites involved in alternative splicing of human immunodeficiency virus type 1 RNA. Virology 213:373-385.
34. Purcell, D. F. J., and M. A. Martin. 1993. Alternative splicing of human immunodeficiency virus type 1 mRNA modulates viral protein expression, replication, and infectivity. J. Virol. 67:6365-6378.
35. Robert-Guroff, M., M. Popovic, S. Gartner, P. Markham, R. C. Gallo, and M. S. Reitz. 1990. Structure and expression of tat-, rev-, and nef-specific transcripts of human immunodeficiency virus type 1 in infected lymphocytes and macrophages, J. Virol. 64:3391-3398.
36. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
37. Schwartz, S., B. Felber, and G. N. Pavlakis. 1991. Expression of human immunodeficiency virus type 1 vif and vpr mRNAs is rev-dependent and regulated by splicing. Virology 183:677-686.
38. Schwartz, S., B. K. Felber, D. M. Benko, E.-M. Fenyö, and G. N. Pavlakis. 1990, Cloning and functional analysis of multiply spliced mRNA species of human immunodeficiency virus type 1. J. Virol. 64:2519-2529.
39. Si, Z., B. A. Amendt, and C. M. Stoltzfus. 1997. Splicing efficiency of HIV-1 tat RNA is determined by both a suboptimal 3′ splice site and a 10 nucleotide exon splicing silencer element located within tat exon 2. Nucleic Acids Res. 25:861-867.
40. Siebel C. W., A. Admon, and D. C. Rio. 1995. Soma-specific expression and cloning of PSI, a negative regulator of P element pre-mRNA splicing. Genes Dev. 9:269-283.
41. Siebel, C. W., R. Kanaar, and D. C. Rio. 1994. Regulation of tissue-specific P-element pre-mRNA splicing requires the RNA-binding protein PSI. Genes Dev. 8:1713-1725.
42. Staffa, A., and A. Cochrane. 1995. Identification of positive and negative splicing regulatory elements within the terminal tat-rev exon of human immunodeficiency virus type 1. Mol. Cell. Biol. 15:4597-4605.
43. Staffa, A., and A. Cochrane. 1994. The tat/rev intron of human immunodeficiency virus type 1 is inefficiently spliced because of suboptimal signals in the 3′ splice site. J. Virol. 68:3071-3079.
44. Staknis, D., and R. Reed. 1994. Direct interactions between pre-mRNA and six U2 small nuclear ribonucleoproteins during spliceosome assembly, Mol. Cell. Biol. 14:2994-3005.
45. Stoltzfus, C. M. 1988. Synthesis and processing of avian sarcoma retrovirus RNA. Adv. Virus Res. 35:1-37.
46. Stoltzfus, C. M., and S. J. Fogarty. 1989. Multiple regions in the Rous sarcoma virus src gene intron act in cis to affect the accumulation of unspliced RNA, J. Virol. 63:1669-1676.
47. Touchman, J. W., I. D'Souza, C. A. Heckman, R. Zhou, N. W. Biggart, and E. C. Murphy, Jr. 1995. Branchpoint and polypyrimidine tract mutations mediating the loss and partial recovery of the Moloney murine sarcoma virus MuSVts110 thermosensitive splicing phenotype. J. Virol. 69:7724-7733.
48. Valcarcel, J., R. Singh, P. D. Zamore, and M. R. Green. 1993. The protein Sex-lethal antagonizes the splicing factor U2AF to regulate alternative splicing of transformer pre-mRNA. Nature 362:171-175.
49. Vilardell, J., and J. R. Warner. 1994. Regulation of splicing at an intermediate step in the formation of the spliceosome. Genes Dev. 8:211-220.
50. Vogt, P. K. 1997. Historical introduction to the general properties of retroviruses, p. 1-26. In J. M. Coffin, S. H. Hughes, and H. E. Varmus (ed.), Retroviruses. Cold Spring Harbor Laboratory Press, Plainview, N.Y.
51. Wentz, M. P., B. E. Moore, M. W. Cloyd, S. M. Berget, and L. A. Donehower. 1997. A naturally arising mutation of a potential silencer of exon splicing in human immunodeficiency virus type 1 induces dominant aberrant splicing and arrests virus production. J. Virol. 71:8542-8551.
52. Zhang, L., and C. M. Stoltzfus. 1995. A suboptimal src 3′ splice site is necessary for efficient replication of Rous sarcoma virus. Virology 206:1099-1107.