The hnRNP A1 protein regulates HIV-1 tat splicing via a novel intron silencer element

EMBO Journal

Volumn 20, Issue 20, 2001, Pages 5748-5758

  Tange, Thomas Ø ^a   Damgaard, Christian K ^a   Guth, Sabine ^b   Valcárcel, Juan ^b   Kjems, Jørgen ^a  

^a AARHUS UNIVERSITY   (Denmark)

^b EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

Author keywords

ASF; hnRNP A1; SF2; Splicing silencer; U2AF

Indexed keywords

CIS ACTING ELEMENT; HETEROGENEOUS NUCLEAR RNA; MESSENGER RNA; RIBONUCLEOPROTEIN; VIRUS RNA;

ALTERNATIVE RNA SPLICING; ARTICLE; EXON; HUMAN IMMUNODEFICIENCY VIRUS 1; INTRON; NONHUMAN; PRIORITY JOURNAL; PROTEIN BLOOD LEVEL; SEQUENCE ANALYSIS; SILENCER GENE;

ALTERNATIVE SPLICING; BASE SEQUENCE; CELL-FREE SYSTEM; ENHANCER ELEMENTS (GENETICS); GENE EXPRESSION REGULATION; GENE PRODUCTS, TAT; GENE SILENCING; HELA CELLS; HETEROGENEOUS-NUCLEAR RIBONUCLEOPROTEIN GROUP A-B; HETEROGENEOUS-NUCLEAR RIBONUCLEOPROTEINS; HIV-1; HUMANS; INTRONS; MACROMOLECULAR SUBSTANCES; MOLECULAR SEQUENCE DATA; NUCLEAR PROTEINS; PROTEIN BINDING; PROTEIN SUBUNITS; RECOMBINANT FUSION PROTEINS; REGULATORY SEQUENCES, NUCLEIC ACID; RIBONUCLEOPROTEIN, U2 SMALL NUCLEAR; RIBONUCLEOPROTEINS; RNA PRECURSORS; RNA, MESSENGER; RNA, VIRAL; SPLICEOSOMES;

HUMAN IMMUNODEFICIENCY VIRUS; HUMAN IMMUNODEFICIENCY VIRUS 1; RNA VIRUSES;

EID: 0035886048     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/20.20.5748     Document Type: Article

References (57)

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2. Presence of exon splicing silencers within human immunodeficiency virus type 1 tat exon 2 and tat-rev exon 3: Evidence for inhibition mediated by cellular factors
3. The splicing factor BBP interacts specifically with the pre-mRNA branchpoint sequence UACUAAC
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10. Exon and intron sequences, respectively, repress and activate splicing of a fibroblast growth factor receptor 2 alternative exon
11. hnRNP A1 recruited to an exon in vivo can function as an exon splicing silencer
12. Inefficient spliceosome assembly and abnormal branch site selection in splicing of an HIV-1 transcript in vitro
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14. Specific commitment of different pre-mRNAs to splicing by single SR proteins
15. General splicing factors SF2 and SC35 have equivalent activities in vitro and both affect alternative 5′ and 3′ splice site selection
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18. Mutation of an RSV intronic element abolishes both U11/U12 snRNP binding and negative regulation of splicing
19. Role of an inhibitory pyrimidine element and polypyrimidine tract binding protein in repression of a regulated α-tropomyosin exon
20. Evidence that sequence-independent binding of highly conserved U2 snRNP proteins upstream of the branch site is required for assembly of spliceosomal complex A
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27. Inhibition by SR proteins of splicing of a regulated adenovirus pre-mRNA
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29. Purification and characterization of pre-mRNA splicing factor SF2 from HeLa cells
30. hnRNP complexes: Composition, structure and function
31. Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins
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33. Modulation of exon skipping and inclusion by heterogeneous nuclear ribonucleoprotein A1 and pre-mRNA splicing factor SF2/ASF
34. Substrate specificities of SR proteins in constitutive splicing are determined by their RNA recognition motifs and composite pre-mRNA exonic elements
35. Intronic sequences and 3′ splice sites control Rous sarcoma virus RNA splicing
36. Intron recognition comes of AGe
37. Synergistic stimulation of HIV-1 rev-dependent export of unspliced mRNA to the cytoplasm by hnRNP A1
38. Two strong 5′ splice sites and competing, suboptimal 3′ splice sites involved in alternative splicing of human immunodeficiency virus type 1 RNA
39. A subset of SR proteins activates splicing of the cardiac troponin T alternative exon by direct interactions with an exonic enhancer
40. Selection and characterization of pre-mRNA splicing enhancers: Identification of novel SR protein-specific enhancer sequences
41. Splicing efficiency of human immunodeficiency virus type 1 tat RNA is determined by both a suboptimal 3′ splice site and a 10 nucleotide exon splicing silencer element located within tat exon 2
42. The exon splicing silencer in human immunodeficiency virus type 1 Tat exon 3 is bipartite and acts early in spliceosome assembly
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44. The tat/rev intron of human immunodeficiency virus type 1 is inefficiently spliced because of suboptimal signals in the 3′ splice site
45. Identification of positive and negative splicing regulatory elements within the terminal tat-rev exon of human immunodeficiency virus type 1
46. General splicing factor SF2/ASF promotes alternative splicing by binding to an exonic splicing enhancer
47. SF2/ASF binds to a splicing enhancer in the third HIV-1 tat exon and stimulates U2AF binding independently of the RS domain
48. The protein Sex-lethal antagonizes the splicing factor U2AF to regulate alternative splicing of transformer pre-mRNA
49. Interaction of U2AF65 RS region with pre-mRNA of branch point and promotion base pairing with U2 snRNA
50. Functional analysis of splicing factors and regulators
51. Intrinsic U2AF binding is modulated by exon enhancer signals in parallel with changes in splicing activity
52. The role of exon sequences in splice site selection
53. A naturally arising mutation of a potential silencer of exon splicing in human immunodeficiency virus type 1 induces dominant aberrant splicing and arrests virus production
54. Specific interactions between proteins implicated in splice site selection and regulated alternative splicing
55. Identification, purification and biochemical characterization of U2 small nuclear ribonucleoprotein auxiliary factor
56. The splicing factor U2AF35 mediates critical protein-protein interactions in constitutive and enhancer-dependent splicing