Exon identity established through differential antagonism between exonic splicing silencer-bound hnRNP A1 and enhancer-bound SR proteins

Molecular Cell

Volumn 8, Issue 6, 2001, Pages 1351-1361

  Zhu, Jun ^a,b   Mayeda, Akila ^c   Krainer, Adrian R ^a  

^a Watson School of Biological Sciences   (United States)

^b STONY BROOK UNIVERSITY   (United States)

^c UNIVERSITY OF MIAMI MILLER SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN; PROTEIN; PROTEIN SR; UNCLASSIFIED DRUG;

ARTICLE; BINDING AFFINITY; BINDING SITE; CONTROLLED STUDY; ENHANCER REGION; EXON; HUMAN; HUMAN CELL; HUMAN IMMUNODEFICIENCY VIRUS; PROTEIN BINDING; PROTEIN PROCESSING; SILENCER GENE;

HUMAN IMMUNODEFICIENCY VIRUS; HUMAN IMMUNODEFICIENCY VIRUS 1;

EID: 0035691667     PISSN: 10972765     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1097-2765(01)00409-9     Document Type: Article

References (43)

1. hnRNP A1 binds promiscuously to oligoribonucleotides: Utilization of random and homo-oligonucleotides to discriminate sequence from base-specific binding
2. Presence of exon splicing silencers within human immunodeficiency virus type 1 tat exon 2 and tat-rev exon 3: Evidence for inhibition mediated by cellular factors
3. RNA splicing at human immunodeficiency virus type 1 3′ splice site A2 is regulated by binding of hnRNP A/B proteins to an exonic splicing silencer element
4. Modulation of exon skipping by high-affinity hnRNP A1-binding sites and by intron elements that repress splice site utilization
5. Exonic splicing enhancers: Mechanism of action, diversity and role in human genetic diseases
6. RNA binding specificity of hnRNP A1: Significance of hnRNP A1 high- affinity binding sites in pre-mRNA splicing
7. Mammalian pre-mRNA splicing factors
8. hnRNP A/B proteins are required for inhibition of HIV-1 pre-mRNA splicing
9. Mammalian heterogeneous nuclear ribonucleoprotein complex protein A1. Large-scale overproduction in Escherichia coli and cooperative binding to single-stranded nucleic acids
10. hnRNP A1 recruited to an exon in vivo can function as an exon splicing silencer
11. Crystal structure of the two-RRM domain of hnRNP A1 (UP1) complexed with single-stranded telomeric DNA
12. Selection of alternative 5′ splice sites: Role of U1 snRNP and models for the antagonistic effects of SF2/ASF and hnRNP A1
13. Specific commitment of different pre-mRNAs to splicing by single SR proteins
14. Sorting out the complexity of SR protein functions
15. 65 in enhancer-dependent splicing
16. Regulated tissue-specific expression of antagonistic pre-mRNA splicing factors
17. Initial sequencing and analysis of the human genome
18. Conserved stem-loop structures in the HIV-1 RNA region containing the A3 3′ splice site and its cis-regulatory element: Possible involvement in RNA splicing
19. Cell type-specific expression of hnRNP proteins
20. On the thermodynamics and kinetics of the cooperative binding of bacteriophage T4-coded gene 32 (helix destabilizing) protein to nucleic acid lattices
21. Purification and characterization of pre-mRNA splicing factor SF2 from HeLa cells
22. Exonic splicing enhancer motif recognized by human SC35 under splicing conditions
23. A mechanism for exon skipping caused by nonsense or missense mutations in BRCA1 and other genes
24. Nuclease protection of RNAs containing site-specific labels: A rapid method for mapping RNA-protein interactions
25. Preparation of HeLa cell nuclear and cytosolic S100 extracts for in vitro splicing
26. Mammalian in vitro splicing assays
27. Function of conserved domains of hnRNP A1 and other hnRNP A/B proteins
28. Substrate specificities of SR proteins in constitutive splicing are determined by their RNA recognition motifs and composite pre-mRNA exonic elements
29. Autogenous regulatory site on the bacteriophage T4 gene 32 messenger RNA
30. Regulation of fibronectin EDA exon alternative splicing: Possible role of RNA secondary structure for enhancer display
31. Interactions of the A1 heterogeneous nuclear ribonucleoprotein and its proteolytic derivative, UP1, with RNA and DNA: Evidence for multiple RNA binding domains and salt-dependent binding mode transitions
32. mRNA splicing efficiency in yeast and the contribution of nonconserved sequences
33. Shuttling of pre-mRNA binding proteins between nucleus and cytoplasm
34. B in the myometrium of the pregnant human uterus: A molecular mechanism for regulating regional protein isoform expression in vivo
35. Mechanisms of fidelity in pre-mRNA splicing
36. Multiple distinct splicing enhancers in the protein-coding sequences of a constitutively spliced pre-mRNA
37. The exon splicing silencer in human immunodeficiency virus type 1 Tat exon 3 is bipartite and acts early in spliceosome assembly
38. Alternative pre-mRNA splicing: The logic of combinatorial control
39. Identification of positive and negative splicing regulatory elements within the terminal tat-rev exon of human immunodeficiency virus type 1
40. Multiple splicing defects in an intronic false exon
41. SF2/ASF binds to a splicing enhancer in the third HIV-1 tat exon and stimulates U2AF binding independently of the RS domain
42. The hnRNP A1 protein regulates HIV-1 tat splicing via a novel intron silencer element
43. Pre-mRNA splicing in the absence of an SR protein RS domain