The sequence complementarity between HIV-1 5′ splice site SD4 and U1 snRNA determines the steady-state level of an unstable env pre-mRNA

RNA

Volumn 7, Issue 3, 2001, Pages 421-434

  Kammler, Susanne ^a   Leurs, Cordula ^a   Freund, Marcel ^a   Krummheuer, Jörg ^a   Seidel, Kerstin ^a   Tange, Thomas Ø ^b   Lund, Mette K ^b   Kjems, Jørgen ^b   Scheid, Andreas ^a   Schaal, Heiner ^a,c  

^a HEINRICH HEINE UNIVERSITY   (Germany)

^b AARHUS UNIVERSITY   (Denmark)

^c Geb 2221   (Germany)

Author keywords

Envelope; ESE; HIV 1; RNA splicing; RNA stability; U1 snRNA

Indexed keywords

MESSENGER RNA PRECURSOR; PROTEIN SD4; SMALL NUCLEAR RNA; UNCLASSIFIED DRUG; VIRUS ENVELOPE PROTEIN; VIRUS PROTEIN;

ARTICLE; GENE EXPRESSION; GENE MUTATION; GENETIC COMPLEMENTATION; HUMAN IMMUNODEFICIENCY VIRUS 1; HYDROGEN BOND; IN VITRO STUDY; OPEN READING FRAME; POINT MUTATION; PRIORITY JOURNAL; PROTEIN MOTIF; RNA SEQUENCE; RNA SPLICING; RNA STABILITY;

BASE PAIR MISMATCH; BASE PAIRING; BASE SEQUENCE; GENE PRODUCTS, ENV; HIV-1; HYDROGEN BONDING; MOLECULAR SEQUENCE DATA; MUTATION; NUCLEAR PROTEINS; NUCLEIC ACID CONFORMATION; RNA PRECURSORS; RNA SPLICING; RNA STABILITY; RNA, SMALL NUCLEAR; RNA, VIRAL;

HUMAN IMMUNODEFICIENCY VIRUS; HUMAN IMMUNODEFICIENCY VIRUS 1; RNA VIRUSES;

EID: 0035031935     PISSN: 13558382     EISSN: None     Source Type: Journal    
DOI: 10.1017/S1355838201001212     Document Type: Article

References (65)

1. Production of acquired immunodeficiency syndrome-associated retrovirus in human and nonhuman cells transfected with an infectious molecular clone
2. Presence of exon splicing silencers within human immunodeficiency virus type 1 tat exon 2 and tat-rev exon 3: Evidence for inhibition mediated by cellular factors
3. Poly(A) site selection in the HIV-1 provirus: Inhibition of promoter-proximal polyadenylation by the downstream major splice donor site
4. The HIV-1 5′ LTR poly(A) site is inactivated by U1 snRNP interaction with the downstream major splice donor site
5. The sequence and context of the 5′ splice site govern the nuclear stability of polyoma virus late RNAs
6. Translocation in yeast and mammalian cells: Not all signal sequences are functionally equivalent
7. Regulation of gene expression by human foamy virus and potentials of foamy viral vectors
8. The conformation of chicken, rat and human U1A RNAs in solution
9. Splicing of precursors to mRNAs by the spliceosomes
10. hnRNP A/B proteins are required for inhibition of HIV-1 pre-mRNA splicing
11. Regulation by HIV Rev depends upon recognition of splice sites
12. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
13. Mechanism of action of regulatory proteins encoded by complex retroviruses
14. The RNA-binding protein TIA-1 is a novel mammalian splicing regulator acting through intron sequences adjacent to a 5′ splice site
15. Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei
16. Inefficient spliceosome assembly and abnormal branch site selection in splicing of an HIV-1 transcript in vitro
17. A short sequence within two purine-rich enhancers determines 5′ splice site specificity
18. HIV-1 regulatory/accessory genes: Keys to unraveling viral and host cell biology
19. The rev gene product of the human immunodeficiency virus affects envelope-specific RNA localization
20. Rev protein of human immunodeficiency virus type 1 affects the stability and transport of the viral mRNA
21. Evidence that HIV-1 Rev directly promotes the nuclear export of unspliced RNA
22. Rev-mediated nuclear export of RNA is dominant over nuclear retention and is coupled to the Ran-GTPase cycle
23. The role of branch-point and 3′-exon sequences in the control of balanced splicing of avian retrovirus RNA
24. Splicing as a requirement for biogenesis of functional 16S mRNA of simian virus 40
25. Splicing and the formation of stable RNA
26. Monomethylated cap structures facilitate RNA export from the nucleus
27. The role of overlapping U1 and U11 5′ splice site sequences in a negative regulator of splicing
28. The ins and outs of HIV Rev
29. A suboptimal 5′ splice site is a cis-acting determinant of nuclear export of polyomavirus late mRNAs
30. Pre-mRNA splicing imprints mRNA in the nucleus with a novel RNA-binding protein that persists in the cytoplasm
31. Control of retroviral RNA splicing through maintenance of suboptimal processing signals
32. Pararetro- and retrovirus RNA: Splicing and the control of nuclear export
33. Specific regulation of mRNA splicing in vitro by a peptide from HIV-1 Rev
34. Protein-protein interactions and 5′-splice-site recognition in mammalian mRNA precursors
35. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
36. A splicing enhancer in the human fibronectin alternate ED1 exon interacts with SR proteins and stimulates U2 snRNP binding
37. Some cis- and trans-acting mutants for splicing target pre-mRNA to the cytoplasm
38. Pre-mRNA splicing alters mRNP composition: Evidence for stable association of proteins at exon-exon junctions
39. U1 small nuclear RNA plays a direct role in the formation of a rev-regulated human immunodeficiency virus env mRNA that remains unspliced
40. Splicing is required for rapid and efficient mRNA export in metazoans
41. The T4 gene encodes the AIDS virus receptor and is expressed in the immune system and the brain
42. Intronic sequences and 3′ splice sites control Rous sarcoma virus RNA splicing
43. Splicing of precursors to mRNA by the spliceosome
44. A catalogue of splice junction sequences
45. Elements distinct from human immunodeficiency virus type 1 splice sites are responsible for the Rev dependence of env mRNA
46. Two strong 5′ splice sites and competing, suboptimal 3′ splice sites involved in alternative splicing of human immunodeficiency virus type 1 RNA
47. Splicing of messenger RNA precursors
48. Interaction of the U1 snRNP with nonconserved intronic sequences affects 5′ splice site selection
49. Mechanisms of fidelity in pre-mRNA splicing
50. Requirement of N-terminal amino acid residues of gp41 for human immunodeficiency virus type 1-mediated cell fusion
51. Env and Vpu proteins of human immunodeficiency virus type 1 are produced from multiple bicistronic mRNAs
52. Human growth hormone as a reporter gene in regulation studies employing transient gene expression
53. RNA splice junctions of different classes of eukaryotes: Sequence statistics and functional implications in gene expression
54. Splicing efficiency of human immunodeficiency virus type 1 tat RNA is determined by both a suboptimal 3′ splice site and a 10 nucleotide exon splicing silencer element located within tat exon
55. The exon splicing silencer in human immunodeficiency virus type 1 Tat exon 3 is bipartite and acts early in spliceosome assembly
56. The tat/rev intron of human immuno-deficiency virus type 1 is inefficiently spliced because of suboptimal signals in the 3′ splice site
57. Identification of positive and negative splicing regulatory elements within the terminal tat-rev exon of human immunodeficiency virus type 1
58. The HIV "A" (sor) gene product is essential for virus infectivity
59. A functional interaction between Rev and yeast pre-mRNA is related to splicing complex formation
60. Determinants of SR protein specificity
61. A naturally arising mutation of a potential silencer of exon splicing in human immunodeficiency virus type 1 induces dominant aberrant splicing and arrests virus production
62. The cardiac troponin T alternative exon contains a novel purine-rich positive splicing element
63. Identification of eight proteins that cross-link to pre-mRNA in the yeast commitment complex
64. A suboptimal src 3′ splice site is necessary for efficient replication of Rous sarcoma virus