Can hepatoma cell lines be redifferentiated to be used in drug metabolism studies?

Alternatives to Laboratory Animals

Volumn 32, Issue SUPPL. 1A, 2004, Pages 65-74

  Martínez Jiménez, Celia P ^a   Jover, Ramiro ^a,b   Gómez Lechón, Maria José ^a   Castell, José V ^a,b  

^a HOSPITAL UNIVERSITARIO LA FE   (Spain)

^b UNIVERSITY OF VALENCIA   (Spain)

Author keywords

Cytochrome P450; Drug metabolism; Hepatoma cell lines; Redifferentiation; Transcription factors

Indexed keywords

CYTOCHROME P450; GROWTH FACTOR; TRANSCRIPTION FACTOR;

CANCER CELL CULTURE; CELL CULTURE; CELL DIFFERENTIATION; CELL IMMORTALIZATION; CELL STRAIN HEPG2; CONFERENCE PAPER; DRUG METABOLISM; HUMAN; HUMAN CELL; LIVER CELL CARCINOMA; ONCOGENE C MYC; ONCOGENE H RAS; ONCOGENE N RAS; PHENOTYPE; PRIORITY JOURNAL; PROTEIN EXPRESSION; SIMIAN VIRUS 40; TRANSGENIC ANIMAL;

ANIMALIA; SIMIAE; SIMIAN VIRUS 40;

EID: 3042565869     PISSN: 02611929     EISSN: None     Source Type: Journal    
DOI: 10.1177/026119290403201s11     Document Type: Conference Paper

References (56)

1. Fischbach, M., Cao, H., Ibañez, M.D., Tsaconas, S., Alouani, S., Montandon, F., el Baraka, M., Padieu, P., Dreano, M. & Chessebeuf-Pasdieu, M. (1991). Maintenance of liver function in long-term culture of hepatocytes following in vitro or in vivo Ha-ras transfection. Cell Biology and Toxicology 7, 327-345.
2. Bayad, J., Bagrel, D., Sabolovic, N., Magdalou, J. & Siest, G. (1991). Expression and regulation of drug metabolizing enzymes in an immortalized rat hepatocyte cell line. Biochemical Pharmacology 12, 1345-1351.
3. Isom, H.C., Woodworth, C.D., Meng, Y., Kreider, J., Miller, T. & Mengel, L. (1992). Introduction of the ras oncogene transforms a simian virus 40-immortalized hepatocyte cell line without loss of expression of albumin and other liver-specific genes. Cancer Research 52, 940-948.
4. Anderson, K., Yin, L.M., MacDonald, C. & Grant, M.H. (1996). Immortalized hepatocytes as in vitro model systems for toxicity testing: the comparative toxicity of menadione in immortalized cells, primary cultures of hepatocytes and HTC hepatoma cells. Toxicology in Vitro 10, 721-727.
5. Ourlin, J.C., Vilarem, M.J., Daujaut, M., Harricane, M.C., Domergue, J., Joyeux, H., Baulieux, J. & Maurel, P. (1997). Lipid-mediated transfection of normal adult human hepatocytes in primary culture. Analytical Biochemistry 247, 34-44.
6. Jover, R., Bort, R., Gómez-Lechón, M.J. & Castell, J.V. (2000). Using hepatic cell lines for drug metabolism studies? In Progress in the Reduction, Refinement and Replacement of Animal Experimentation (ed. M. Balls, A-M. van Zeller & M.E. Halder), pp. 127-137. Amsterdam, The Netherlands: Elsevier Science B.V.
7. Paul, D., Hohne, M., Pinkert, C., Piasecki, A., Ummelmann, E. & Brinster, R. (1998). Immortalized differentiated hepatocyte lines derived from transgenic mice harboring SV40 T-antigen genes. Experimental Cell Research 175, 354-362.
8. Yanai, N., Suzuki, M. & Obinata, M. (1991). Hepatocyte cell lines established from transgenic mice harboring temperature-sensitive simian virus 40 large T antigen gene. Experimental Cell Research 197, 50-56.
9. Wu, J.C., Merlino, G., Cveklova, K., Mosinger, B. & Fausto, N. (1994). Autonomous growth in serum-free medium production of hepatocellular carcinomas by differentiated hepatocyte lines that overexpress transforming growth factor alpha 1. Cancer Research 54, 5964-5973.
10. Amicone, L., Spagnoli, F.M., Spath, G., Giordano, S., Tommasini, C., Bernardini, S., De Luca, V., Della Rocca, C., Weiss, M.C., Comoglio, P.M. & Tripodi, M. (1997). Transgenic expression in the liver of truncated Met blocks apoptosis and permits immortalization of hepatocytes. EMBO Journal 16, 495-503.
11. Bulera, S.J., Haas, M.J., Sattler, C.A. & Pitot, H.C. (1997). Cell lines with heterogenous phenotypes from a single isolation of albumin-SV40 T-antigen transgenic rat hepatocytes. Hepatology 25, 1192-1203.
12. Courjault-Gautier, F., Antoine, B., Bens, M., Vallet, V., Cluzeaud, F., Pringault, E., Khan, A., Toutain, H. & Vandewalle, A. (1997). Activity and inducibility of drug-metabolizing enzymes in immortalized hepatocyte-like cells (mhPKT) derived from a L-PK/Tag1 transgenic mouse. Experimental Cell Research 234, 362-372.
13. Donato, M.T., Klocke, R., Castell, J.V., Stenzel, K., Paul, D. & Gómez-Lechón, M.J. (2003). Constitutive and inducible expression of CYP isozymes in immortal hepatocytes derived from SV40 transgenic mice. Xenobiotica 33, 459-473.
14. Klocke, R., Gómez-Lechón, M.J., Ehrhardt, A., Mendoza-Figueroa, T., Donato, T., López-Revilla, R., Castell, J.V. & Paul, D. (2002). Establishment and characterization of immortal hepatocytes derived from various transgenic mouse lines. Biochemical and Biophysical Research Communications 294, 864-871.
15. Rodríguez-Antona, C., Donato, M.T., Boobis, A., Edwards, R.J., Watts, P.S., Castell, J.V. & Gómez-Lechón, M.J. (2002). Cytochrome P-450 expression in human hepatocytes and human hepatoma cell lines. Molecular mechanisms that determine the lower expression in cultured cells. Xenobiotica 32, 505-520.
16. Donato, M.T., Bassi, A.M., Gómez-Lechón, M.J., Penco, S., Herrero, E., Adamo, D., Castell, J.V. & Ferro, M. (1994). Evaluation of the xenobiotic biotransformation capability of six rodent hepatoma cell lines in comparison with rat hepatocytes. In Vitro Cellular and Developmental Biology 30A, 574-580.
17. Gómez-Lechón, M.J., Donato, T., Jover, R., Rodriguez, C., Ponsoda, X., Glaise, D., Castell, J.V. & Guguen-Guillouzo, C. (2001). Expression and induction of a large set of drug metabolism enzymes by the highly differentiated human hepatoma cell line BC2. European Journal of Biochemistry 268, 1448-1459.
18. Rodriguez-Antona, C., Jover, R., Gómez-Lechón, M.J. & Castell, J.V. (2000). Quantitative RT-PCR measurement of human cytochrome P-450s: application to drug induction studies. Archives of Biochemistry and Biophysics 375, 1-8.
19. Cereghini, S. (1996). Liver-enriched transcription factors and hepatocyte differentiation. FASEB Journal 10, 267-282.
20. Jover, R., Bort, R., Gómez-Lechón, M.J. & Castell, J.V. (1998). Re-expression of C/EBP alpha induces CYP2B6, CYP2C9 and CYP2D6 genes in Hep G2 cells. FEBS Letters 431, 227-230.
21. Castell, J.V., Hernandez, D., Gomez-Foix, A.M., Guillen, I., Donato, T. & Gómez-Lechón, M.J. (1997). Adenovirus-mediated gene transfer into human hepatocytes: analysis of the biochemical functionality of transduced cells. Gene Therapy 4, 455-464.
22. Rodriguez-Antona, C., Bort, R., Jover, R., Tindberg, N., Ingelman-Sundberg, M., Gómez-Lechón, M.J. & Castell, J.V. (2002). Transcriptional regulation of human CYP3A4 expression by C/EBPα and HNF3γ. Molecular Pharmacology 63, 1180-1189.
23. Lai, E., Clark, K., Burley, S. & Darnell, J. (1993). Hepatocyte nuclear factor 3/fork head or "winged helix" proteins: a family of transcription factors of diverse biologic function. Proceedings of the National Academy of Sciences USA 90, 10421-10423.
24. Kaestner, K., Hiemisch, H. & Schutz, G. (1998). Targeted disruption of the gene encoding hepatocyte nuclear factor 3gamma results in reduced transcription of hepatocyte-specific genes. Molecular and Cellular Biology 18, 4245-4251.
25. Crowe, A., Sang, L., Li, K., Lee, K., Spear, B. & Barton, M. (1999). Hepatocyte nuclear factor 3 relieves chromatin-mediated repression of the alpha-fetoprotein gene. Journal of Biological Chemistry 274, 25113-25120.
26. Donato, T., Jover, R., Ponsoda, X., Castell, J. & Gómez-Lechón, M. (2002). In vitro hepatic models for drug metabolism studies and human cytochrome P450 characterization. Recent Research in Developmental Drug Metabolism and Disposition 1, 83-100.
27. Mangelsdorf, D., Thummel, C., Beato, M., Herrlich, P., Schutz, G., Umesono, K., Blumberg, B., Kastner, P., Mark, M., Chambon, P., et al. (1995). The nuclear receptor superfamily: the second decade. Review 83, 835-839.
28. Hertz, R., Magenheim, J., Berman, I. & Bar, T.J. (1998). Fatty acyl-CoA thioesters are ligands of hepatic nuclear factor-4alpha. Nature, London 392, 512-516.
29. Sladek, F., Zhong, W., Lai, E. & Darnell, J.J. (1990). Liver-enriched transcription factor HNF-4 is a novel member of the steroid hormone receptor superfamily. Genes and Development 12B, 2353-2365.
30. Jiang, G., Nepomuceno, L., Hopkins, K. & Sladek, F. (1995). Exclusive homodimerization of the orphan receptor hepatocyte nuclear factor 4 defines a new subclass of nuclear receptors. Molecular and Cellular Biology 9, 5131-5143.
31. Yamagata, K., Furuta, H., Oda, N., Kaisaki, P., Menzel, S., Cox, N., Fajans, S., Signorini, S., Stoffel, M. & Bell, G. (1996). Mutations in the hepatocyte nuclear factor-4alpha gene in maturity-onset diabetes of the young (MODY1). Nature, London 384, 458-460.
32. Hadzopoulou-Cladaras, M., Kistanova, E., Evagelopoulou, C., Zeng, S., Cladaras, C. & Ladias, J. (1997). Functional domains of the nuclear receptor hepatocyte nuclear factor 4. Journal of Biological Chemistry 1, 539-550.
33. Jover, R., Bort, R., Gómez-Lechón, M.J. & Castell, J.V. (2001). Cytochrome P450 regulation by hepatocyte nuclear factor 4 in human hepatocytes: a study using adrenovirus-mediated antisense targeting. Hepatology 33, 668-675.
34. Nakhei, H., Lingott, A., Lemm, I. & Ryffel, G. (1998). An alternative splice variant of the tissue specific transcription factor HNF4alpha predominates in undifferentiated murine cell types. Nucleic Acids Research 2, 497-504.
35. Chartier, F., Bossu, J., Laudet, V., Fruchart, J. & Laine, B. (1994). Cloning and sequencing of cDNAs encoding the human hepatocyte nuclear factor 4 indicate the presence of two isoforms in human liver. Gene 2, 269-272.
36. Hata, S., Tsukamoto, T. & Osumi, T. (1992). A novel isoform of rat hepatocyte nuclear factor 4 (HNF-4). Biochimica et Biophysica Acta 1131, 211-213.
37. Hata, S., Inoue, T., Kosuga, K., Nakashima, T., Tsukamoto, T. & Osumi, T. (1995). Identification of two splice isoforms of mRNA for mouse hepatocyte nuclear factor 4 (HNF-4). International Journal of Peptide and Protein Research 2, 106-112.
38. Drewes, T., Senkel, S., Holewa, B. & Ryffel, G. (1996). Human hepatocyte nuclear factor 4 isoforms are encoded by distinct and differentially expressed genes. Molecular and Cellular Biology 3, 925-931.
39. Furuta, H., Iwasaki, N., Oda, N., Hinokio, Y., Horikawa, Y., Yamagata, K., Yano, N., Sugahiro, J., Ogata, M., Ohgawara, H., Omori, Y., Iwamoto, Y. & Bell, G. (1997). Organization and partial sequence of the hepatocyte nuclear factor-4 alpha/MODY1 gene and identification of a missense mutation, R127W, in a Japanese family with MODY. Diabetes 46, 1652-1657.
40. Torres-Padilla, M., Fougere-Deschatrette, C. & Weiss, M. (2001). Expression of HNF4alpha isoforms in mouse liver development is regulated by sequential promoter usage and constitutive 3′ end splicing. Mechanisms of Development 2, 183-193.
41. Kimura, A., Nishiyori, A., Murakami, T., Tsukamoto, T., Hata, S., Osumi, T., Okamura, R., Mori, M. & Takiguchi, M. (1993). Chicken ovalbumin upstream promoter-transcription factor (COUP-TF) represses transcription from the promoter of the gene for ornithine transcarbamylase in a manner antagonistic to hepatocyte nuclear factor-4 (HNF-4). Journal of Biological Chemistry 268, 11125-11133.
42. Ktistaki, E. & Talianidis, I. (1997). Modulation of hepatic gene expression by hepatocyte nuclear factor 1. Science, New York 277, 109-112.
43. Miyajima, N., Kadowaki, Y., Fukushige, S., Shimizu, S., Semba, K., Yamanashi, Y., Matsubara, K., Toyoshima, K. & Yamamoto, T. (1988). Identification of two novel members of erbA superfamily by molecular cloning: the gene products of the two are highly related to each other. Nucleic Acids Research 16, 11057-11074.
44. Descombes, P. & Schibler, U. (1991). A liver-enriched transcriptional activator protein, LAP, and a transcriptional inhibitory protein, LIP, are translated from the same mRNA. Cell 67, 569-579.
45. Ossipow, V., Descombes, P. & Schibler, U. (1993). CCAAT/enhancer-binding protein mRNA is translated into multiple proteins with different transcription activation potentials. Proceedings of the National Academy of Sciences USA 90, 8219-8223.
46. Welm, A., Mackey, S., Timchenko, L., Darlington, G. & Timchenko, N. (2000). Translational induction of liver-enriched transcriptional inhibitory protein during acute phase response leads to repression of CCAAT/enhancer binding protein alpha mRNA. Journal of Biological Chemistry 275, 27406-27413.
47. Zahnow, C., Younes, P., Lauciric, R. & Rosen, J. (1997). Overexpression of C/EBPbeta-LIP, a naturally occurring dominant-negative transcription factor, in human breast cancer. Journal of the National Cancer Institute 89, 1887-1891.
48. Trautwein, C., Rakemann, T., Pietrangelo, A., Plumpe, J., Montosi, G. & Manns, M. (1996). C/EBP-beta/LAP controls down-regulation of albumin gene transcription during liver regeneration. Journal of Biological Chemistry 271, 22262-22270.
49. Jover, R., Bort, R., Gómez-Lechón, M.J. & Castell, J. (2002). Down-regulation of human CYP3A4 by the inflammatory signal interleukin-6: molecular mechanism and transcription factors involved. FASEB Journal 16, 1799-1801.
50. Ron, D. & Habener, J. (1992). CHOP, a novel developmentally regulated nuclear protein that dimerizes with transcription factors C/EBP and LAP and functions as a dominant-negative inhibitor of gene transcription. Genes and Development 6, 439-453.
51. Tang, Q. & Lane, M. (2000). Role of C/EBP homologous protein (CHOP-10) in the programmed activation of CCAAT/enhancer-binding protein-beta during adipogenesis. Proceedings of the National Academy of Sciences USA 97, 12446-12450.
52. Razin, A. & Riggs, A. (1980). DNA methylation and gene function. Science 210, 604-610.
53. Jones, P., Veenstra, G., Wade, P., Vermaak, D., Kass, S., Landsberger, N., Strouboulis, J. & Wolffe, A. (1998). Methylated DNA and MeCP2 recruit histone deacetylase to repress transcription. Nature Genetics 19, 187-191.
54. Nan, X., Ng, H., Johnson, C., Laherty, C., Turner, B., Eisenman, R. & Bird, A. (1998). Transcriptional repression by the methyl-CpG-binding protein MeCP2 involves a histone deacetylase complex. Nature, London 393, 386-389.
55. Collingwood, T., Urnov, F. & Wolffe, A. (1999). Nuclear receptors: coactivators, corepressors and chromatin remodeling in the control of transcription. Journal of Molecular Endocrinology 23, 255-275.
56. Brown, C., Lechner, T., Howe, L. & Workman, J. (2000). The many HATs of transcription coactivators. Trends in Biochemical Sciences 25, 15-19.