Establishment and characterization of immortal hepatocytes derived from various transgenic mouse lines

Biochemical and Biophysical Research Communications

Volumn 294, Issue 4, 2002, Pages 864-871

  Klocke, Rainer ^a,e   Jose Gomez Lechon M ^b   Ehrhardt, Anja ^a,f   Mendoza Figueroa, Tomas ^c   Teresa Donato, M ^b   López Revilla, Rubén ^c,d   Castell, José V ^b   Paul, Dieter ^a  

^a FRAUNHOFER INSTITUTE FOR TOXICOLOGY AND EXPERIMENTAL MEDICINE   (Germany)

^b HOSPITAL UNIVERSITARIO LA FE   (Spain)

^c DEPARTAMENTO DE FÍSICA   (Mexico)

^d INSTITUTO POTOSINO DE INVESTIGACIÓN CIENTÍFICA Y TECNOLÓGICA   (Mexico)

^e Ingenium Pharmaceuticals AG   (Germany)

^f STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Differentiation; Epidermal growth factor; Immortalization; Mouse hepatocytes; p53 Knockout; SV40 T antigen

Indexed keywords

ARGININE; CYTOCHROME P450; EPIDERMAL GROWTH FACTOR; LIVER ENZYME; MESSENGER RNA; PLASMA PROTEIN; PROTEIN P53; TESTOSTERONE; TRANSCRIPTION FACTOR; VIRUS ANTIGEN;

ANIMAL CELL; ANIMAL EXPERIMENT; ANTIGEN EXPRESSION; ARTICLE; BIOTECHNOLOGY; CELL CULTURE; CELL DIFFERENTIATION; CELL IMMORTALIZATION; CELL ISOLATION; CELL LINE; CONTROLLED STUDY; CULTURE MEDIUM; DNA MODIFICATION; FEMALE; GENETIC CODE; KNOCKOUT MOUSE; LIVER CELL; LIVER FUNCTION; MALE; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; SEQUENCE HOMOLOGY; SIMIAN VIRUS 40; TESTOSTERONE METABOLISM; TRANSGENIC MOUSE;

ANIMALS; BLOTTING, WESTERN; CELL LINE; CHROMOSOMES; CULTURE MEDIA, SERUM-FREE; CYTOCHROME P-450 ENZYME SYSTEM; HEPATOCYTES; MICE; MICE, INBRED BALB C; MICE, KNOCKOUT; MICE, TRANSGENIC; MICROSCOPY, PHASE-CONTRAST; NEOPLASM TRANSPLANTATION; PROTEIN ISOFORMS; RNA, MESSENGER; TUMOR SUPPRESSOR PROTEIN P53;

MUS MUSCULUS;

EID: 0036289805     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-291X(02)00579-X     Document Type: Article

References (36)

1. The use of cultured hepatocytes to investigate the mechanisms of drug hepatotoxicity
2. Developments in bioartificial liver research: Concepts performance and applications
3. Long-term expression of differentiated functions in hepatocytes cultured in three-dimensional collagen matrix
4. Epidermal growth factor- and hepatocyte growth factor-receptor activity in serum-free cultures of human hepatocytes
5. Liver cell models in in vitro toxicology
6. Characterization of the asialoglycoprotein receptor in a continuous hepatoma line
7. Transformation of differentiated neonatal rat hepatocytes in primary culture by polyoma virus early region sequences
8. Immortalized differentiated hepatocyte lines derived from transgenic mice harboring SV40 T-antigen genes
9. Cell lines with heterogeneous phenotypes result from a single isolation of albumin-SV40 T-antigen transgenic rat hepatocytes
10. Detection of mechanisms of action of carcinogens: Immortalization and malignant transformation of differentiated cells in vitro and in transgenic mice
11. Precocious induction of tyrosine amino-transferase mRNA by hydrocortisone in cultured fetal rat hepatocytes at different developmental stages
12. Responsiveness of an SV40-immortalized hepatocyte cell line to growth hormone
13. Immortalization and malignant transformation of hepatocytes by transforming genes of polyoma virus and of SV40 virus in vitro and in vivo
14. Activity and inducibility of drug-metabolizing enzymes in immortalized hepatocyte-like cells (mhPKT) derived from a L-PK/Tag1 transgenic mouse
15. Establishment and characterization of differentiated nontransformed hepatocyte cell lines derived from mice transgenic for transforming growth factor alpha
16. Proliferation of fetal rat hepatocytes in response to growth factors and hormones in primary culture
17. Autocrine mitogen IgEGF cooperates with c-myc or with the Hcs locus during hepatocarcinogenesis in transgenic mice
18. Mice deficient for p53 are developmentally normal but susceptible to spontaneous tumours
19. SV40 enhancer and large-T antigen are instrumental in development of choroid plexus tumours in transgenic mice
20. Serum dependent growth of primary cultured differentiated fetal rat hepatocytes in arginine-deficient medium
21. Cultivation of adult rat hepatocytes on 3T3 cells: Expression of various liver differentiated functions
22. Transcriptional control in hepatocytes of normal and c14CoS albino deletion mice
23. Role of innervation excitability and myogenic factors in the expression of the muscular chloride channel ClC-1. A study on normal and myotonic muscle
24. Stimulation of de novo synthesis of cytochrome P-450 by phenobarbital in primary nonproliferating cultures of adult rat hepatocytes
25. Expression and induction of a large set of drug metabolism enzymes by the highly differentiated human hepatoma cell line BC2
26. A novel female-specific member of the CYP3A gene subfamily in the mouse liver
27. The murine Ah locus
28. Cytochrome P450 isoenzyme activities in cultured rat and mouse liver slices
29. Enhanced proliferative potential in culture of cells from p53 deficient mice
30. Establishment of immortal hepatocytes from a CYP3A7-transgenic/p53 knockout mouse
31. P53 Deficiency in liver reduces local control of survival and proliferation but does not affect apoptosis after DNA damage
32. P53 the cellular gatekeeper for growth and division
33. Sex-associated expression of mouse hepatic and renal CYP2B enzymes by glucocorticoid hormones
34. Liver-enriched transcription factors and hepatocyte differentiation
35. P53 Transformation-related protein: Detection of an associated phosphotransferase activity
36. Time-sensitive reversal of hyperplasia in transgenic mice expressing SV40 T antigen