Transgenic expression in the liver of truncated Met blocks apoptosis and permits immortalization of hepatocytes

EMBO Journal

Volumn 16, Issue 3, 1997, Pages 495-503

  Amicone, Laura ^a   Spagnoli, Francesca M ^a   Späth, Gerald ^b   Giordano, Silvia ^c   Tommasini, Cristina ^a   Bernardini, Silvia ^a   De Luca, Veronica ^a   Della Rocca, Carlo ^d   Weiss, Mary C ^b   Comoglio, Paolo M ^c   Tripodi, Marco ^a  

^a Pasteur Institute   (Italy)

^b CNRS   (France)

^c UNIVERSITY OF TURIN   (Italy)

^d SAPIENZA UNIVERSITY OF ROME   (Italy)

Author keywords

Apoptosis protection; Cell polarity; Immortalized hepatocytes; Met

Indexed keywords

COMPLEMENTARY DNA; LIVER PROTEIN; ONCOPROTEIN; SCATTER FACTOR; TRANSCRIPTION FACTOR;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; APOPTOSIS; ARTICLE; CELL DIFFERENTIATION; CELL IMMORTALIZATION; CELL MOTILITY; CELL PROLIFERATION; CONTROLLED STUDY; CYTOPLASM; EXPRESSION VECTOR; FETUS; LIVER; LIVER CELL; MALE; MOUSE; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROTO ONCOGENE; TRANSGENE;

ALPHA 1-ANTITRYPSIN; ANIMALS; ANTIBODIES, MONOCLONAL; APOPTOSIS; BLOTTING, NORTHERN; BLOTTING, SOUTHERN; BLOTTING, WESTERN; CELL DIFFERENTIATION; CELLS, CULTURED; GENE EXPRESSION REGULATION, DEVELOPMENTAL; HEPATOCYTE GROWTH FACTOR; HISTOCYTOCHEMISTRY; HUMANS; IMMUNOHISTOCHEMISTRY; LIVER; MICE; MICE, TRANSGENIC; MICROSCOPY, ELECTRON; PROTO-ONCOGENES; RECEPTOR PROTEIN-TYROSINE KINASES; TRANSGENES;

ANIMALIA;

EID: 0031032004     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/16.3.495     Document Type: Article

References (64)

1. Amicone, L., Galimi, M.A., Spagnoli, F.M., Tommasini, C., De Luca, V. and Tripodi, M. (1995) Temporal and tissue-specific expression of the MET ORF driven by the complete transcriptional unit of human A1AT gene in transgenic mice. Gene, 162, 323-329.
2. Antoine, B., Levrat, F., Valle, V., Berbar, T., Cartier, N., Dubois, N., Briant, P. and Kahn, A. (1992) Gene expression in hepatocyte-like lines established by targeted carcinogenesis in transgenic mice. Exp. Cell Res., 200, 175-185.
3. Bardelli, A., Longati, P., Albero, D., Goruppi, S., Schneider, C., Ponzetto, C. and Comoglio, P.M. (1996) HGF receptor associates with the anti-apoptotic protein BAG-1 and prevents cell death. EMBO J., 15, 6025-6212.
4. Barre, B.A., Hart, J.K., Coles, H.S.R., Burne, J.F., Voyvodic, J.T., Richardson, W.D. and Raff, M.C. (1992) Cell death and control of cell survival in the oligodendrocyte lineage. Cell, 70, 31-46.
5. Bishop, J.M. (1991) Molecular themes in oncogenesis. Cell, 64, 235-248.
6. Bottaro, D.P., Rubin, J.S., Faletto, D.L., Chan, A.M.L., Kmiecik, T.E., Vande Woude, G.F. and Aaronson, S.A. (1991) Identification of the hepatocyte growth factor receptor as the c-met proto-oncogene product. Science, 251, 802-804.
7. Bussolino, F. et al. (1992) Hepatocyte growth factor is a potent angiogenic factor which stimulates endothelial cell motility and growth. J. Cell Biol., 119, 629-641.
8. Cao, Z., Umek, R.M. and McKnight, S.L. (1991) Regulated expression of three C/EBP isoforms during adipose conversion of 3T3-L1 cells. Genes Dev., 5, 1538-1552.
9. Cassio, D. and Weiss, M.C. (1979) Expression of fetal and neonatal hepatic functions by mouse hepatoma-rat hepatoma hybrids. Somat. Cell Genet., 5, 719-738.
10. Chiarugi, V., Magnelli, L. and Ruggiero, M. (1994) Apoptosis, senescence, immortalization and cancer. Pharmacol. Res., 30, 301-315.
11. Cooper, C.S., Park, M. Blair, D.G., Tainsky, M.A., Huebner, K., Croce, C.M. and Van Woude, G.F. (1984) Molecular cloning of a new transforming gene from a chemically transformed human cell. Nature, 311, 29-33.
12. Crabtree, G.R. and Kant, J.A. (1981) Molecular cloning of cDNA for the alpha, beta, and gamma chains of rat fibrinogen. A family of coordinately regulated genes. J. Biol. Chem., 256, 9718-9723.
13. DeFrances, M.C., Wolf, H.K., Michalopoulos, G.K. and Zarnegar, R. (1992) The presence of hepatocyte growth factor in the developing rat. Development, 116, 387-395.
14. De Simone, V., De Magistris, L., Lazzaro, D., Gerstner, J., Monaci, P., Nicosia, A. and Cortese, R. (1991) LFB3, a heterodimer-forming homeoprotein of the LFB1 family, is expressed in specialized epithelia. EMBO J., 10, 1435-1443.
15. Frain, M., Swart, G., Monaci, P., Nicosia, A., Stampfli, S., Frank, R. and Cortese, R. (1989) The liver-specific transcription factor LF-B1 contains a highly diverged homeobox DNA binding domain. Cell, 59, 145-157.
16. Frish, S.M. and Francis, H. (1994) Disruption of epithelial cell-matrix interactions induces apoptosis. J. Cell Biol., 124, 619-626.
17. Giordano, S., Ponzetto, C., Di Renzo, M.F., Cooper, C.S. and Comoglio, P.M. (1989) Tyrosine kinase receptor indistinguishable from the c-met protein. Nature, 339, 155-156.
18. Giordano, S., Zhen, Z., Medico, E., Gaudino, G., Galimi, F. and Comoglio, P.M. (1993) Transfer of motogenic and invasive response to scatter factor/hepatocyte growth factor by transfection of human MET protooncogene. Proc. Natl Acad. Sci. USA, 90, 649-653.
19. Gottardi, C.J. and Caplan, M.J. (1993) An ion-transporting ATPase encodes multiple apical localization signals. J. Cell Biol., 121, 283-293.
20. Graziani, A., Gramaglia, D., Cantley, L.C. and Comoglio, P.M. (1991) The tyrosine-phosphorylated hepatocyte growth factor/scatter factor receptor associates with phosphatidylinositol 3-kinase. J. Biol. Chem., 266, 22087-22090.
21. Harrington, E.A., Bennet, M.R., Fanidi, A. and Evan, G.I. (1994) C-Myc-induced apoptosis in fibroblast is inhibited by specific cytokines. EMBO J., 13, 3286-3295.
22. Harvey, M. et al. (1993) In vitro characteristics of embryo fibroblasts isolated from p-53 deficient mice. Oncogene, 8, 2457-2467.
23. Hockenbery, D., Nunez, G., Milliman, C., Schreiber, R.D. and Korsmeyer, S.J. (1990) Bcl-2 is an inner mithochondrial membrane protein that blocks programmed cell death. Nature, 348, 334-336.
24. Jacobson, M.D., Burne, J.F. and Raff, M.C. (1994) Programmed cell death and Bcl-2 protection in the absence of a nucleus. EMBO J., 13, 1899-1910.
25. Landschulz, W.H., Johnson, P.F., Adashi, E.Y., Graves, B.J. and McKnight, S.L. (1988) Isolation of a recombinant copy of the gene encoding C/EBP. Genes Dev., 2, 786-800.
26. Mével-Ninio, M. and Weiss, M.C. (1981) Immunofluorescent analysis of the time course of extinction, re-expression and activation of albumin production in heterocaryons and hybrids of rat hepatoma cells with mouse fibroblast. J. Cell Biol., 90, 339-350.
27. Michalopoulos, G.K. (1990) Liver regeneration: molecular mechanisms of growth control. FASEB J., 4, 176-187.
28. Montesano, R., Matsumoto, K., Nakamura, T. and Orci, L. (1991) Identification of a fibroblast-derived epithelial morphogen as hepatocyte growth factor. Cell, 67, 901-908.
29. Naldini, L., Vigna, E., Narsimhan, R.P., Gaudino, G., Zarnegar, R., Michalopoulos, G.K. and Comoglio, P.M. (1991a) Hepatocyte growth factor (HGF) stimulates the tyrosine kinase activity of the receptor encoded by the proto-oncogene c-met. Oncogene, 6, 501-504.
30. Naldini, L. et al. (1991b) Scatter factor and hepatocyte growth factor are indistinguishable ligands for the met receptor. EMBO J., 10, 2867-2878.
31. Nakamura, T., Teramoto, H. and Ichihara, A. (1986) Purification and characterization of a growth factor from rat platelets for mature parenchymal hepatocytes in primary cultures. Proc. Natl Acad. Sci. USA, 83, 6489-6493.
32. Ni, R., Tomita, Y. Matsuda, K., Ichihara, A., Ishimura, K., Ogasawara, J. and Nagata, S. (1994) Fas-mediated apoptosis in primary cultured mouse hepatocytes. Exp. Cell. Res., 215, 332-337.
33. Nunez, G., London, L., Hockenbery, D., Alexander, M., McKearn, J.P. and Korsmeyer, S.J. (1990) Deregulated Bcl-2 gene expression selectively prolongs survival of growth factor-deprived hemopoietic cell lines. J. Immunol., 144, 3602-3610.
34. Ogasawara, J., Watanabe-Fukunaga, R., Adachi, M., Matsuzawa, A., Kasugai, T., Kitamura, Y., Itoh, N., Suda, T. and Nagata, S. (1993) Lethal effect of the anti-Fas antibody in mice. Nature, 364, 806-809.
35. Overturf, K., Al-Dhalimy, M., Tanguay, R., Brantly, M., Ou, C.N., Finegold, M. and Grompe, M. (1996) Hepatocytes corrected by gene therapy are selected in vivo in a murine model of hereditary tyrosinaemia type I. Nature Genet., 12, 266-273.
36. Park, M., Dean, M., Cooper, C.S., Schmidt, M., O'Brien, S.J., Blair, D.G. and Vande Woude, G.F. (1986) Mechanism of met oncogene activation. Cell, 45, 895-904.
37. Paul, D.L. (1986) Molecular cloning of cDNA for rat liver gap junction protein. J. Cell Biol., 103, 123-134.
38. Poli, V., Mancini, F.P. and Cortese, R. (1990) IL-6DBP, a nuclear protein involved in interleukin-6 signal transduction, defines a new family of leucine zipper proteins related to C/EBP. Cell, 63, 643-653.
39. Ponzetto, C., Bardelli, A., Maina, F., Longati, P., Panayotou, G., Dhand, R., Waterfield, M.D. and Comoglio, P.M. (1993) A novel recognition motif for phosphatidylinositol 3-kinase binding mediates its association with the hepatocyte growth factor/scatter factor receptor. Mol. Cell. Biol., 13, 4600-4608.
40. Ponzetto, C., Bardelli, A., Zhen, Z., Maina, F., Dalla Zonca, P., Giordano, S., Graziani, A., Panayotou, G. and Comoglio, P.M. (1994) A multifunctional docking site mediates signalling and transformation by the hepatocyte growth factor/scatter factor receptor family. Cell, 77, 261-271.
41. Raff, M.C. (1992) Social controls on cell survival and cell death. Nature, 356, 397-400.
42. Raff, M.C., Barres, B.A., Burne, J.F., Coles, H.S., Ishizaki, Y. and Jacobson, M.D. (1993) Programmed cell death and the control of cell survival: lessons from the nervous system. Science, 262, 695-700.
43. Rodrigues, G.A. and Park, M. (1993) Dimerization mediated through a leucine zipper activates the oncogenic potential of the met receptor tyrosine kinase. Mol. Cell. Biol., 13, 6711-6722.
44. Rubin, J.S. et al. (1991) A broad-spectrum human lung fibrobast-derived mitogen is a variant of hepatocyte growth factor. Proc. Natl Acad. Sci. USA, 88, 415-419.
45. Rüther, U., Tripodi, M., Cortese, R. and Wagner, E. (1987) The human α-1-antitrypsin gene is efficiently expressed from two promoters in transgenic mice. Nucleic Acids Res., 15, 7519-7529.
46. Sargent, T.D., Wu, J.R., Sala-Trepar, J.M., Wallace, R.B., Reyes, A.A. and Bonner, J. (1979) The rat serum albumin gene: analysis of cloned sequence. Proc. Natl Acad. Sci. USA, 76, 3256-3260.
47. Sawyers, C.L. and Denny, C.T. (1994) Chronic myelomonocytic leukaemia: tel-a-kinase what ets all about. Cell, 77, 171-173.
48. Schmidt, C., Bladt, E., Goedecke, S., Brinkmann, V., Zschiesche, W., Sharpe, M., Gherardi, E. and Birchmeier, C. (1995) Scatter factor/ hepatocyte growth factor is essential for liver development. Nature, 373, 699-702.
49. Shay, J.W., Pereira-Smith, O.M. and Wright, W.E. (1991) A role for both RB and p53 in the regulation of human cellular senescence. Exp. Cell. Res., 196, 33-39.
50. Sigal, S.H., Brill, S., Fiorino, A. and Reid, L.M. (1992) The liver as a stem cell and lineage system. Am. J. Physiol., 263, G139-G148.
51. Skala, H., Vibert, M., Lamas, E., Maire, P., Schweighoffer, F. and Kahn, A. (1987) Molecular cloning and expression of rat aldolase C messenger RNA during development and hepatocarcinogenesis. Eur. J. Biochem., 163, 513-518.
52. Sladek, F.M., Zhong, W.M., Lai, E. and Darnell, J.E.,Jr (1990) Liver enriched transcription factor HNF-4 is a novel member of the steroid hormone receptor superfamily. Genes Dev., 4, 2353-2365.
53. Snider, W.D. (1994) Functions of the neurotrophins during nervous system development: what the knockouts are teaching us. Cell, 77, 627-638.
54. Stern, C.D., Ireland, G.W., Herrick, S.E., Gherardi, E., Gray, J., Perryman, M. and Stoker, M. (1990) Epithelial scatter factor and development of the chick embryonic axis. Development, 110, 1271-1284.
55. Stevenson, B.R., Siciliano, J., Mooseker, M.S. and Goodenough, D.A. (1986) Identification of ZO-1: a high molecular weight polypeptide associated with the tight function (zonula occludens) in a variety of epithelia. J. Cell Biol., 103, 755-766.
56. Takayama, S., Sato, T., Krajewski, S., Kochel, K., Irie, S., Millan, J.A. and Reed, J.C. (1995) Cloning and functional analysis of BAG-1: a novel Bcl-2-binding protein with anti-cell death activity. Cell, 80, 279-284.
57. Tsukada, T. et al. (1993) Enhanced proliferate potential in culture of cells from p53-deficient mice. Oncogene, 8, 3313-3322.
58. Van der Geer, P., Hunter, T. and Lindberg, R.A. (1994) Receptor protein-tyrosine kinases and their signal transduction pathways. Annu. Rev. Cell Biol., 10, 251-337.
59. Vaux, D.L., Cory, S. and Adams, J.M. (1988) Bcl-2 gene promotes haemopoietic cell survival and cooperates with c-myc to immortalize pre-B cells. Nature, 335, 440-442.
60. Weidner, K.M., Behrens, J., Vandekerckove, J. and Birchmeier, W. (1990) Scatter factor: molecular characteristics and effect on the invasiveness of epithelial cells. J. Cell Biol., 111, 2097-2108.
61. Wu, J.C., Merlino, G., Cveklova, K., Mosinger, B.,Jr and Fausto, N. (1994a) Autonomous growth in serum-free medium and production of hepatocellular carcinomas by differentiated hepatocyte lines that overexpress transforming growth factor alpha 1. Cancer Res., 54, 5964-5973.
62. Wu, J.C., Merlino, G. and Fausto, N. (1994b) Establishment and characterization of differentiated, non transformed hepatocyte cell lines derived from mice transgenic for transforming growth factor alpha. Proc. Natl Acad. Sci. USA, 91, 674-678.
63. Yao, R. and Cooper, G.M. (1995) Requirement for phosphatidylinositol-3 kinase in the prevention of apoptosis by nerve growth factor. Science, 267, 2003-2006.
64. Zhen, Z., Giordano, S., Longati, P., Medico, E., Campiglio, M. and Comoglio, P.M. (1994) Structural and functional domains critical for constitutive activation of the HGF-receptor (Met). Oncogene, 9, 1691-1697.