Observation of individual microtubule motor steps in living cells with endocytosed quantum dots

Journal of Physical Chemistry B

Volumn 109, Issue 51, 2005, Pages 24220-24224

  Nan, Xiaolin ^a   Sims, Peter A ^a   Chen, Peng ^a,b   Sunney Xie, X ^a  

^a HARVARD UNIVERSITY   (United States)

^b Department of Population Medicine and Diagnostic Sciences   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOLOGICAL ORGANS; PHOTOGRAPHY; PROTEINS; SEMICONDUCTOR QUANTUM DOTS;

DYNEIN MOVEMENTS; ENDOCYTOSED QUANTUM DOTS; MICROTUBULE MOTOR STEPS; MOTOR DISPLACEMENT TRACES; QD ASSAY;

CELLS;

EID: 30344436982     PISSN: 15206106     EISSN: None     Source Type: Journal    
DOI: 10.1021/jp056360w     Document Type: Article

References (36)

1. For reviews, see (a) Schliwa, M.; Woehlke, G. Molecular motors. Nature (London) 2003, 422, 759-765;
2. (b) Vale, R. D. The molecular toolbox for intracellular transport. Cell 2003, 112, 467-480 and references therein.
3. Asbury, C. L.; Fehr, A. N.; Block, S. M. Kinesin moves by an asymmetric hand-over-hand mechanism. Science 2003, 302, 2130-2134.
4. Burgess, S. A.; Walker, M. L.; Sakakibara, H.; Knight, P. J.; Oiwa, K. Dynein structure and power stroke. Nature (London) 2003, 421, 715-718.
5. Hua, W.; Young, E. C.; Fleming, M. L.; Gelles, J. Coupling of kinesin steps to ATP hydrolysis. Nature (London) 1997, 388, 390-393.
6. Schnitzer, M. J.; Visscher, K.; Block, S. M. Force production by single kinesin motors. Nat. Cell Biol. 2000, 2, 718-723.
7. Mallik, R.; Carter, B. C.; Lex, S. A.; King, S. J.; Gross, S. P. Cytoplasmic dynein functions as a gear in response to load. Nature (London) 2004, 427, 649-652.
8. Ashkin, A.; Schutze, K.; Dziedzic, J. M.; Euteneuer, U.; Schliwa, M. Force generation of organelle transport measured in vivo by an infrared laser trap. Nature (London) 1990, 348, 346-348.
9. Howard, J. Mechanics of Motor Proteins and the Cytoskeleton; Sinauer Associates, Inc.: Sunderland, Massachusetts, 2001.
10. Weite, M. A.; Gross, S. P.; Postner, M.; Block, S. M.; Wieschaus, E. F. Developmental regulation of vesicle transport in Drosophila embryos: forces and kinetics. Cell 1998, 92, 547-557.
11. Rural, C.; Kim, H.; Syed, S.; Goshima, G.; Gelfand, V. I.; Selvin, P. R. Kinesin and dynein move a peroxisome in vivo: a tug-of-war or coordinated movement? Science 2005, 308, 1469-1472.
12. King, S. J.; Schroer, T. A. Dynactin increases the processivity of the cytoplasmic dynein motor. Nat. Cell Biol. 2000, 2, 20-24.
13. Gross, S. P. Dynactin: coordinating motors with opposite inclinations. Curr. Biol. 2003, 13, R320-R322.
14. Chan, W. C.; Nie, S. Quantum dot bioconjugates for ultrasensitive nonisotopic detection. Science 1998, 281, 2016-2018.
15. Bruchez, M., Jr.; Moronne, M.; Gin, P.; Weiss, S.; Alivisatos, A. P. Semiconductor nanocrystals as fluorescent biological labels. Science 1998, 287, 2013-2016.
16. Jaiswal, J. K.; Mattoussi, H.; Mauro, J. M.; Simon, S. M. Long-term multiple color imaging of live cells using quantum dot bioconjugates. Nat. Biotechnol. 2003, 21, 47-51.
17. Smith, A. M.; Gao, X.; Nie, S. Quantum dot nanocrystals for in vivo molecular and cellular imaging. Photochem. Photobiol. 2004, 80, 377-385.
18. Warshaw, D. M.; Kennedy, G. G.; Work, S. S.; Krementsova, E. B.; Beck, S.; Trybus, K. M. Differential labeling of myosin V heads with quantum dots allows direct visualization of hand-over-hand processivity. Biophys. J. 2005, 88, L30-L32.
19. Thompson, R. E.; Larson, D. R.; Webb, W. W. Precise nanometer localization analysis for individual fluorescent probes. Biophys. J. 2002, 82, 2775-2783.
20. Yildiz, A.; Forkey, J. N.; McKinney, S. A.; Ha, T.; Goldman, Y. E.; Selvin, P. R. Myosin V walks hand-over-hand: single fluorophore imaging with 1.5-nm localization. Science 2003, 300, 2061-2065.
21. Yildiz, A.; Tomishige, M.; Vale, R. D.; Selvin, P. R. Kinesin walks hand-over-hand. Science 2004, 303, 676-678.
22. Jaiswal, J. K.; Goldman, E. R.; Mattoussi, H.; Simon, S. M. Use of quantum dots for live cell imaging. Nat. Methods 2004, 1, 73-78.
23. Osaki, P.; Kanamori, T.; Sando, S.; Sera, T.; Aoyama, Y. A quantum dot conjugated sugar ball and its cellular uptake. On the size effects of endocytosis in the subviral region. J. Am. Chem. Soc. 2004, 126, 6520-6521.
24. Chung, S. H.; Kennedy, R. A. Forward-backward nonlinear filtering technique for extracting small biological signals from noise. J. Neurosci. Methods 1991, 40, 71-86.
25. Coppin, C. M.; Pierce, D. W.; Hsu, L.; Vale, R. D. The load dependence of kinesin's mechanical cycle. Proc. Natl. Acad. Sci. USA 1997, 94, 8539-8544.
26. Hohng, S.; Ha, T. Near-complete suppression of quantum dot blinking in ambient conditions. J. Am. Chem. Soc. 2004, 126, 1324-1325.
27. In reference 10, the authors studied peroxisomes moving in the processes of Drosophila "S2" cells at 10°C, which is a different system from ours.
28. Kural, C.; Balci, H.; Selvin, P. R. Molecular motors one at a time: FIONA to the rescue. J. Phys.: Condens. Matter 2005, 17, S3979-S3995.
29. Noda, Y.; Okada, Y.; Saito, N.; Setou, M.; Xu, Y.; Zhang, Z.; Hirokawa, N. KIFC3, a microtubule minus end-directed motor for the apical transport of annexin XIIIb-associated Triton-insoluble membranes. J. Cell Biol. 2001, 155, 77-88.
30. Burkhardt, J. K.; Echeverri, C. J.; Nilsson, T.; Vallee, R. B. Overexpression of the dynamitin (p50) subunit of the dynactin complex disrupts dynein-dependent maintenance of membrane organelle distribution. J. Cell Biol. 1997, 139, 469-484.
31. Yamada, S.; Wirtz, D.; Kuo, S. C. Mechanics of living cells measured by laser tracking microrheology. Biophys. J. 2000, 78, 1736-1747.
32. Burgess, S. A.; Walker, M. L.; Thirumurugan, K.; Trinick, J.; Knight, P. J. Use of negative stain and single-particle image processing to explore dynamic properties of flexible macromolecules. J. Struct. Biol. 2004, 147, 247-258.
33. Burgess, S. A.; Knight, P. J. Is the dynein motor a winch? Curr. Opin. Struct. Biol. 2004, 14, 138-146.
34. Burgess, S. A.; Walker, M. L.; Sakakibara, H.; Oiwa, K.; Knight, P. J. The structure of dynein-c by negative stain electron microscopy. J. Struct. Biol. 2004, 146, 205-216.
35. King, S. M. AAA domains and organization of the dynein motor unit. J. Cell Sci. 2000, 113(Pt 14), 2521-2526.
36. Hackney, D. D. The kinetic cycles of myosin, kinesin, and dynein. Annu. Rev. Physiol. 1996, 58, 731-750.