The structure of dynein-c by negative stain electron microscopy

Journal of Structural Biology

Volumn 146, Issue 1-2, 2004, Pages 205-216

  Burgess, S A ^a   Walker, M L ^a   Sakakibara, H ^b   Oiwa, K ^b   Knight, P J ^a  

^a UNIVERSITY OF LEEDS   (United Kingdom)

^b NATIONAL INSTITUTE OF INFORMATION AND COMMUNICATIONS TECHNOLOGY   (Japan)

Author keywords

AAA protein; Axoneme; Cilia; Dynein; Electron microscopy; Flagella; Molecular motor

Indexed keywords

DYNEIN ADENOSINE TRIPHOSPHATASE; NUCLEOTIDE;

BINDING AFFINITY; CONFERENCE PAPER; CRYOELECTRON MICROSCOPY; DENSITY; ELECTRON MICROSCOPY; ENZYME STRUCTURE; MICROTUBULE; MORPHOLOGY; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN DOMAIN; STAINING;

ANIMALS; CHLAMYDOMONAS REINHARDTII; DYNEIN ATPASE; MICROSCOPY, ELECTRON; MODELS, MOLECULAR; MOLECULAR MOTOR PROTEINS; PROTOZOAN PROTEINS;

EID: 1642321108     PISSN: 10478477     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jsb.2003.10.005     Document Type: Conference Paper

References (47)

1. Asai D.J., Koonce M.P. The dynein heavy chain: structure, mechanics and evolution. Trends Cell Biol. 11:2001;196-202.
2. Burgess S.A. Rigor and relaxed outer dynein arms in replicas of cryofixed motile flagella. J. Mol. Biol. 250:1995;52-63.
3. Burgess S.A., Dover S.D., Woolley D.M. Architecture of the outer arm dynein ATPase in an avian sperm flagellum, with further evidence for the B-link. J. Cell Sci. 98(Pt 1):1991;17-26.
4. Burgess S.A., Carter D.A., Dover S.D., Woolley D.M. The inner dynein arm complex: compatible images from freeze-etch and thin section methods of microscopy. J. Cell Sci. 100(Pt 2):1991;319-328.
5. Burgess S.A., Walker M.L., White H.D., Trinick J. Flexibility within myosin heads revealed by negative stain and single-particle analysis. J. Cell Biol. 139:1997;675-681.
6. Burgess S.A., Walker M.L., Sakakibara H., Knight P.J., Oiwa K. Dynein structure and power stroke. Nature. 421:2003;715-718.
7. DiBella L.M., King S.M. Dynein motors of the Chlamydomonas flagellum. Int. Rev. Cytol. 210:2001;227-268.
8. Fan J., Amos L.A. Antibodies to cytoplasmic dynein heavy chain map the surface and inhibit motility. J. Mol. Biol. 307:2001;1317-1327.
9. Frank J. Three-Dimensional Electron Microscopy of Macromolecular Assemblies. 1996;Academic Press, New York.
10. Gee M., Vallee R. The role of the dynein stalk in cytoplasmic and flagellar motility. Eur. Biophys. J. 27:1998;466-473.
11. Gee M.A., Heuser J.E., Vallee R.B. An extended microtubule-binding structure within the dynein motor domain. Nature. 390:1997;636-639.
12. Gibbons I.R. Dynein family of motor proteins: present status and future questions. Cell Motil. Cytoskeleton. 32:1995;136-144.
13. Gibbons I.R., Gibbons B.H., Mocz G., Asai D.J. Multiple nucleotide-binding sites in the sequence of dynein beta heavy chain. Nature. 352:1991;640-643.
14. Gibbons I.R., Lee-Eiford A., Mocz G., Phillipson C.A., Tang W.J., Gibbons B.H. Photosensitized cleavage of dynein heavy chains. Cleavage at the "V1 site" by irradiation at 365. nm in the presence of ATP and vanadate J. Biol. Chem. 262:1987;2780-2786.
15. Goodenough U., Heuser J. Structural comparison of purified dynein proteins with in situ dynein arms. J. Mol. Biol. 180:1984;1083-1118.
16. Goodenough U.W., Heuser J.E. Substructure of the outer dynein arm. J. Cell Biol. 95:1982;798-815.
17. Goodenough U.W., Heuser J.E. Outer and inner dynein arms of cilia and flagella. Cell. 41:1985;341-342.
18. Goodenough U.W., Heuser J.E. Substructure of inner dynein arms, radial spokes, and the central pair/projection complex of cilia and flagella. J. Cell Biol. 100:1985;2008-2018.
19. Goodenough U.W., Gebhart B., Mermall V., Mitchell D.R., Heuser J.E. High-pressure liquid chromatography fractionation of Chlamydomonas dynein extracts and characterization of inner-arm dynein subunits. J. Mol. Biol. 194:1987;481-494.
20. Harrison A., King S.M. The molecular anatomy of dynein. Essays Biochem. 35:2000;75-87.
21. Johnson K.A. Pathway of the microtubule-dynein ATPase and the structure of dynein: a comparison with actomyosin. Annu. Rev. Biophys. Biophys. Chem. 14:1985;161-188.
22. Karki S., Holzbaur E.L. Cytoplasmic dynein and dynactin in cell division and intracellular transport. Curr. Opin. Cell Biol. 11:1999;45-53.
23. King S.M. AAA domains and organization of the dynein motor unit. J.Cell Sci. 113:2000;2521-2526.
24. King S.M. The dynein microtubule motor. Biochim. Biophys. Acta. 1496:2000;60-75.
25. King S.M. Organization and regulation of the dynein microtubule motor. Cell Biol. Int. 27:2003;213-215.
26. Kliche W., Fujita-Becker S., Kollmar M., Manstein D.J., Kull F.J. Structure of a genetically engineered molecular motor. EMBO J. 20:2001;40-46.
27. Koonce M.P., Samso M. Overexpression of cytoplasmic dynein's globular head causes a collapse of the interphase microtubule network in Dictyostelium. Mol. Biol. Cell. 7:1996;935-948.
28. Koonce M.P., Tikhonenko I. Functional elements within the dynein microtubule-binding domain. Mol. Biol. Cell. 11:2000;523-529.
29. LeDizet M., Piperno G. The light chain p28 associates with a subset of inner dynein arm heavy chains in Chlamydomonas axonemes. Mol. Biol. Cell. 6:1995;697-711.
30. Mocz G., Gibbons I.R. Phase partition analysis of nucleotide binding to axonemal dynein. Biochemistry. 35:1996;9204-9211.
31. Mocz G., Gibbons I.R. Model for the motor component of dynein heavy chain based on homology to the AAA family of oligomeric ATPases. Structure (Camb). 9:2001;93-103.
32. Mocz G., Helms M.K., Jameson D.M., Gibbons I.R. Probing the nucleotide binding sites of axonemal dynein with the fluorescent nucleotide analogue. 2′(3′) - O -(- N -methylanthraniloyl)-adenosine 5′ -triphosphate Biochemistry. 37:1998;9862-9869.
33. Neuwald A.F., Aravind L., Spouge J.L., Koonin E.V. AAA+: a class of chaperone-like ATPases associated with the assembly, operation, and disassembly of protein complexes. Genome Res. 9:1999;27-43.
34. Ogawa K. Four ATP-binding sites in the midregion of the beta heavy chain of dynein. Nature. 352:1991;643-645.
35. Okuno M. Inhibition and relaxation of sea urchin sperm flagella by vanadate. J. Cell Biol. 85:1980;712-725.
36. Sakakibara H., Kojima H., Sakai Y., Katayama E., Oiwa K. Inner-arm dynein c of Chlamydomonas flagella is a single-headed processive motor. Nature. 400:1999;586-590.
37. Sale W.S., Goodenough U.W., Heuser J.E. The substructure of isolated and in situ outer dynein arms of sea urchin sperm flagella. J. Cell Biol. 101:1985;1400-1412.
38. Samso M., Radermacher M., Frank J., Koonce M.P. Structural characterization of a dynein motor domain. J. Mol. Biol. 276:1998;927-937.
39. Shiroguchi K., Toyoshima Y.Y. Regulation of monomeric dynein activity by ATP and ADP concentrations. Cell Motil. Cytoskeleton. 49:2001;189-199.
40. Silvanovich A., Li M.G., Serr M., Mische S., Hays T.S. The third P-loop domain in cytoplasmic dynein heavy chain is essential for dynein motor function and ATP-sensitive microtubule binding. Mol. Biol. Cell. 14:2003;1355-1365.
41. Vale R.D., Milligan R.A. The way things move: looking under the hood of molecular motor proteins. Science. 288:2000;88-95.
42. Vallee R.B., Gee M.A. Make room for dynein. Trends Cell Biol. 8:1998;490-494.
43. Walker M.L., Burgess S.A., Sellers J.R., Wang F., Hammer 3rd J.A., Trinick J., Knight P.J. Two-headed binding of a processive myosin to F-actin. Nature. 405:2000;804-807.
44. White H.D., Walker M.L., Trinick J. A computer-controlled spraying-freezing apparatus for millisecond time-resolution electron cryomicroscopy. J. Struct. Biol. 121:1998;306-313.
45. Woolley D.M. Studies on the eel sperm flagellum. I. The structure of the inner dynein arm complex. J. Cell Sci. 110(Pt 1):1997;85-94.
46. Yagi T. ADP-dependent microtubule translocation by flagellar inner-arm dyneins. Cell Struct. Funct. 25:2000;263-267.
47. Yanagisawa H.A., Kamiya R. Association between actin and light chains in Chlamydomonas flagellar inner-arm dyneins. Biochem. Biophys. Res. Commun. 288:2001;443-447.