Cytoplasmic dynein functions as a gear in response to load

Nature

Volumn 427, Issue 6975, 2004, Pages 649-652

  Mallik, Roop ^a   Carter, Brian C ^a   Lex, Stephanie A ^b   King, Stephen J ^b   Gross, Steven P ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF MISSOURI KANSAS CITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINETRIPHOSPHATE; HYDROLYSIS; POLYSTYRENES; RNA; VIRUSES;

MOLECULAR MOTORS; NANOMACHINES;

GENETIC ENGINEERING;

ADENOSINE TRIPHOSPHATE; CARRIER PROTEIN; CYTOPLASM PROTEIN; CYTOSKELETON PROTEIN; DYNEIN ADENOSINE TRIPHOSPHATASE; KINESIN; MESSENGER RNA; MICROSPHERE; MOLECULAR MOTOR; NANOPARTICLE; POLYSTYRENE;

BIOLOGY;

ANIMAL TISSUE; ARTICLE; CATTLE; ENERGY METABOLISM; FORCE; MECHANICAL LOAD; MICROTUBULE; MOLECULAR MODEL; MOTION; NONHUMAN; OPTICAL TRAPPING; OPTICS; PRIORITY JOURNAL; PROTEIN FAMILY; PROTEIN FUNCTION; PROTEIN STRUCTURE; WEIGHT BEARING;

ADENOSINE TRIPHOSPHATE; ANIMALS; BIOMECHANICS; CATTLE; CYTOPLASM; DYNEIN ATPASE; HYDROLYSIS; MODELS, BIOLOGICAL; MOLECULAR MOTOR PROTEINS; WEIGHT-BEARING;

ANIMALIA; BOS TAURUS; RNA VIRUSES;

EID: 1342325553     PISSN: 00280836     EISSN: None     Source Type: Journal    
DOI: 10.1038/nature02293     Document Type: Article

References (30)

1. Hirokawa, N. Kinesin and dynein superfamily proteins and the mechanism of organelle transport. Science 279, 519-526 (1998).
2. Hackney, D. D. The kinetic cycles of myosin, kinesin and dynein. Annu. Rev. Physiol. 58, 731-750 (1996).
3. Asai, D. J. & Koonce, M. P. The dynein heavy chain: structure, mechanics and evolution. Trends Cell Biol. 11, 196-202 (2001).
4. King, S. M. AAA domains and organization of the dynein motor unit. J. Cell Sci. 113, 2521-2526 (2000).
5. King, S. M. The dynein microtubule motor. Biochim. Biophys. Acta 1496, 60-75 (2000).
6. Rice, S. E. & Spudich, J. A. Building and using optical traps to study properties of molecular motors. Methods Enzymol. 361, 112-133 (2003).
7. Wang, Z., Khan, S. & Sheetz, M. P. Single cytoplasmic dynein molecule movements: characterization and comparison with kinesin. Biophys. J. 69, 2011-2023 (1995).
8. Svoboda, K. & Block, S. M. Force and velocity measured for single kinesin molecules. Cell 77, 773-784 (1994).
9. Gelles, J., Schnapp, B. J. & Sheetz, M. P. Tracking kinesin-driven movements with nanometre-scale precision. Nature 331, 450-453 (1988).
10. Visscher, K. & Block, S. M. Versatile optical traps with feedback control. Methods Enzymol. 298, 460-489 (1998).
11. Visscher, K., Schnitzer, M. J. & Block, S. M. Single kinesin molecules studied with a molecular force clamp. Nature 400, 184-189 (1999).
12. Sakakibara, H., Kojima, H., Sakai, Y., Katayama, E. & Oiwa, K. Inner-arm dynein c of Chlamydomonas flagella is a single-headed processive motor. Nature 400, 586-590 (1999).
13. Gross, S. P., Welte, M. A., Block, S. M. & Wieschaus, E. F. Dynein-mediated cargo transport in vivo: A switch controls travel distance. J. Cell Biol. 148, 945-955 (2000).
14. Svoboda, K., Schmidt, C. F., Schnapp, B. J., & Block, S. M. Direct observation of kinesin stepping by optical trapping interferometry. Nature 365, 721-727 (1993).
15. Burgess, S. A., Walker, M. L., Sakakibara, H., Knight, P. J. & Oiwa, K. Dynein structure and power stroke. Nature 421, 715-718 (2003).
16. Hua, W., Young, E. C., Fleming, M. L. & Gelles, J. Coupling of kinesin steps to ATP hydrolysis. Nature 388, 390-393 (1997).
17. Hirakawa, E., Higuchi, H. & Toyoshima, Y. Y. Processive movement of single 22S dynein molecules occurs only at low ATP concentrations. Proc. Natl Acad. Sci. USA 97, 2533-2537 (2000).
18. Shingyoji, C., Higuchi, H., Yoshimura, M., Katayama, E. & Yanagida, T. Dynein arms are oscillating force generators. Nature 393, 711-714 (1998).
19. Schnitzer, M. J. & Block, S. M. Kinesin hydrolyses one ATP per 8-nm step. Nature 388, 386-390 (1997).
20. Mehta, A. D. et al. Myosin-V is a processive actin-based motor. Nature 400, 590-593 (1999).
21. +: a class of chaperone-like ATPases associated with the assembly, operation and disassembly of protein complexes. Genome Res. 9, 27-43 (1999).
22. Vale, R. D. AAA proteins. Lords of the ring. J. Cell Biol. 150, F13-F19 (2000).
23. Samso, M., Radermacher, M., Frank, J. & Koonce, M. P. Structural characterization of a dynein motor domain. J. Mol. Biol. 276, 927-937 (1998).
24. Silvanovich, A., Li, M., Serr, M., Mische, S. & Hays, T. S. The third P-loop domain in cytoplasmic dynein heavy chain is essential for dynein motor function and ATP-sensitive microtubule binding. Mol. Biol. Cell 14, 1355-1365 (2003).
25. Mocz, G. & Gibbons, I. R. Model for the motor component of dynein heavy chain based on homology to the AAA family of oligomeric ATPases. Structure 9, 93-103 (2001).
26. Whiteheart, S. W. et al. N-ethylmaleimide-sensitive fusion protein: a trimeric ATPase whose hydrolysis of ATP is required for membrane fusion. J. Cell Biol. 126, 945-954 (1994).
27. Bingham, J. B., King, S. J. & Schroer, T. A. Purification of dynactin and dynein from brain tissue. Methods Enzymol. 298, 171-184 (1998).
28. Schroer, T. A. & Sheetz, M. P. Two activators of microtubule-based vesicle transport. J. Cell Biol. 115, 1309-1318 (1991).
29. Sloboda, R. D. & Rosenbaum, J. L. Purification and assay of microtubule-associated proteins (MAPs). Methods Enzymol. 85, 171-184 (1982).
30. King, S. J. & Schroer, T. A. Dynactin increases the processivity of the cytoplasmic dynein motor. Nature Cell Biol. 2, 20-24 (2000).