Nucleotide exchange from the high-affinity ATP-binding site in SecA is the rate-limiting step in the ATPase cycle of the soluble enzyme and occurs through a specialized conformational state

Biochemistry

Volumn 43, Issue 23, 2004, Pages 7307-7327

  Fak, John J ^a,c   Itkin, Anna ^a,d   Ciobanu, Daita D ^a   Lin, Edward C ^a   Song, Xiang Jin ^a   Chou, Yi Te ^b,e   Gierasch, Lila M ^b   Hunt, John F ^a  

^a Och Spine at New York Presbyterian Hospitals   (United States)

^b UNIVERSITY OF MASSACHUSETTS   (United States)

^c ROCKEFELLER UNIVERSITY   (United States)

^d WEIZMANN INSTITUTE OF SCIENCE   (Israel)

^e MICHIGAN STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINETRIPHOSPHATE; CONFORMATIONS; ENZYMES; GROUND STATE; THERMODYNAMICS;

BINDING SITES; CONFORMATIONAL TRANSITIONS; NUCLEOTIDE EXCHANGE; TRANSLOCATION;

BIOCHEMISTRY;

2' O (N METHYLANTHRANILOYL)ADENOSINE 5' DIPHOSPHATE; ADENINE NUCLEOTIDE; ADENOSINE DIPHOSPHATE; ADENOSINE TRIPHOSPHATASE; MAGNESIUM ION; MEMBRANE PROTEIN; NUCLEOTIDE; PROTEIN SECA; PROTEIN SECYEG; UNCLASSIFIED DRUG;

ARTICLE; BINDING AFFINITY; BINDING SITE; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; ENZYME METABOLISM; HYDROLYSIS; KINETICS; NONHUMAN; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN FOLDING; PROTEIN INTERACTION; PROTEIN TRANSPORT; STEADY STATE; THERMODYNAMICS;

ADENOSINE DIPHOSPHATE; ADENOSINE TRIPHOSPHATASES; ADENOSINE TRIPHOSPHATE; ADENYLYL IMIDODIPHOSPHATE; ANTHRANILIC ACIDS; BACTERIAL PROTEINS; BINDING SITES; KINETICS; MAGNESIUM; MEMBRANE PROTEINS; MEMBRANE TRANSPORT PROTEINS; MUTATION; PROTEIN CONFORMATION; SERINE ENDOPEPTIDASES; SOLUBILITY; TEMPERATURE;

EID: 2942588637     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi0357208     Document Type: Article

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