Helicase structure and mechanism

Current Opinion in Structural Biology

Volumn 12, Issue 1, 2002, Pages 123-133

  Caruthers, Jonathan M ^a   McKay, David B ^a  

^a STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATE; HELICASE; ISOPROTEIN; OLIGONUCLEOTIDE; PROTEIN; PROTEIN RUVB; RNA HELICASE; UNCLASSIFIED DRUG;

CATALYSIS; DATA BASE; ENZYME ACTIVITY; ENZYME MECHANISM; ENZYME STRUCTURE; HEPATITIS C VIRUS; HYDROLYSIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN DNA BINDING; PROTEIN DOMAIN; PROTEIN FAMILY; PROTEIN FUNCTION; PROTEIN MOTIF; PROTEIN RNA BINDING; REVIEW;

HEPATITIS C VIRUS; RNA VIRUSES;

EID: 0036468702     PISSN: 0959440X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0959-440X(02)00298-1     Document Type: Review

References (42)

1. Helicases: Amino acid sequence comparisons and structure-function relationships
2. Distantly related sequences in the alpha- and beta-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold
3. Mechanical devices of the spliceosome: Motors, clocks, springs, and things
4. Modulation of the helicase activity of eIF4A by eIF4B, eIF4H, and eIF4F
5. +: A class of chaperone-like ATPases associated with the assembly, operation, and disassembly of protein complexes
6. Crystal structure of a DExx box DNA helicase
7. Structure of the recA protein-ADP complex
8. Major domain swiveling revealed by the crystal structures of complexes of E. coli Rep helicase bound to single-stranded DNA and ADP
9. Structure of the hepatitis C virus RNA helicase domain
10. Comparisons between the structures of HCV and Rep helicases reveal structural similarities between SF1 and SF2 super-families of helicases
11. Crystal structure of the DNA nucleotide excision repair enzyme UvrB from Thermus thermophilus
12. Crystal structure of Thermus thermophilus HB8 UvrB protein, a key enzyme of nucleotide excision repair
13. Crystal structure of UvrB, a DNA helicase adapted for nucleotide excision repair
14. DExD/H box RNA helicases: From generic motors to specific dissociation functions
15. Crystal structure of the ATPase domain of translation initiation factor 4A from Saccharomyces cerevisiae - The prototype of the DEAD box protein family
16. Crystallographic structure of the amino terminal domain of yeast initiation factor 4A, a representative DEAD-box RNA helicase
17. Crystal structure of yeast initiation factor 4A, a DEAD-box RNA helicase
18. Crystal structure of a DEAD box protein from the hyperthermophile Methanococcus jannaschii
19. Bacteriophage T7 helicase/primase proteins form rings around single-stranded DNA that suggest a general structure for hexameric helicases
20. Crystal structure of the helicase domain from the replicative helicase-primase of bacteriophage T7
21. Crystal structure of T7 gene 4 ring helicase indicates a mechanism for sequential hydrolysis of nucleotides
22. Crystal structure of the hexameric replicative helicase RepA of plasmid RSF1010
23. Two different oligomeric states of the RuvB branch migration motor protein as revealed by electron microscopy
24. Structure and mechanism of the RuvB Holliday junction branch migration motor
25. Crystal structure of the Holliday junction migration motor protein RuvB from Thermus thermophilus HB8
26. Crystal structures of complexes of PcrA DNA helicase with a DNA substrate indicate an inchworm mechanism
27. The structure of the E. coli RecA protein monomer and polymer
28. The Pfam Protein Families Database
29. DNA binding mediates conformational changes and metal ion coordination in the active site of PcrA helicase
30. Crystal structure of RNA helicase from genotype 1b hepatitis C virus. A feasible mechanism of unwinding duplex RNA
31. Hepatitis C virus NS3 RNA helicase domain with a bound oligonucleotide: The crystal structure provides insights into the mode of unwinding
32. Molecular views of viral polyprotein processing revealed by the crystal structure of the hepatitis C virus bifunctional protease-helicase
33. Mutational analysis of a DEAD box RNA helicase: The mammalian translation initiation factor eIF-4A
34. Site-directed mutagenesis of motif III in PcrA helicase reveals a role in coupling ATP hydrolysis to strand separation
35. Defining the roles of individual residues in the single-stranded DNA binding site of PcrA helicase
36. The HRIGRXXR region of the DEAD box RNA helicase eukaryotic translation initiation factor 4A is required for RNA binding and ATP hydrolysis
37. Characterisation of Bacillus stearothermophilus PcrA helicase: Evidence against an active rolling mechanism
38. Uncoupling DNA translocation and helicase activity in PcrA: Direct evidence for an active mechanism
39. Demonstration of unidirectional single-stranded DNA translocation by PcrA helicase: Measurement of step size and translocation speed
40. A two-site kinetic mechanism for ATP binding and hydrolysis by E. coli Rep helicase dimer bound to a single-stranded oligodeoxynucleotide
41. MOLSCRIPT: A program to produce both detailed and schematic plots of protein structures
42. Raster3D: Photorealistic molecular graphics