Genetic dissection of SecA: Suppressor mutations against the secY205 translocase defect

Genes to Cells

Volumn 5, Issue 12, 2000, Pages 991-999

  Matsumoto, G ^a   Nakatogawa, H ^a   Mori, H ^a   Ito, K ^a  

^a KYOTO UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATASE; AMINO ACID; CARRIER PROTEIN;

ALLELE; AMINO ACID SEQUENCE; ARTICLE; CLASSIFICATION; COLD SENSITIVITY; CONTROLLED STUDY; DISSECTION; DOWN REGULATION; ENZYME BINDING; ENZYME DEFECT; GENE ACTIVITY; GENE INSERTION; GENE ISOLATION; GENE MUTATION; GENE REPRESSION; NONHUMAN; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN DOMAIN; PROTEIN MOTIF; PROTEIN PROTEIN INTERACTION; SUPPRESSOR GENE;

ADENOSINE TRIPHOSPHATASES; ADENOSINE TRIPHOSPHATE; ALLELES; AMINO ACID SUBSTITUTION; BACTERIAL PROTEINS; CARRIER PROTEINS; COLD; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENES, BACTERIAL; GENES, SUPPRESSOR; MEMBRANE TRANSPORT PROTEINS; MUTAGENESIS; PROTEIN TRANSPORT;

BACTERIA (MICROORGANISMS);

EID: 0034485424     PISSN: 13569597     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2443.2000.00388.x     Document Type: Article

References (33)

1. Distinct catalytic roles of the SecYE, SecG and SecDFyajC subunits of preprotein translocase holoenzyme
2. SecA membrane cycling at SecYEG is driven by distinct ATP binding and hydrolysis events and is regulated by SecD and SecF
3. SecA promotes preprotein translocation by undergoing ATP-driven cycles of membrane insertion and deinsertion
4. Both an N-terminal 65-kDa domain and a C-terminal 30-kDa domain of SecA cycle into the membrane at SecYEG during translocation
5. The binding cascade of SecB to SecA to SecY/E mediates preprotein targeting to the E. coli plasma membrane
6. Suppression of signal sequence defects and azide resistance in Escherichia coli commonly result from the same mutation in. SecA
7. Conditional-lethal mutations that suppress genetic defects in morphogenesis by altering structural proteins
8. A molecular switch in SecA protein couples ATP hydrolysis to protein translocation
9. The ATPase activity of SecA is regulated by acidic phospholipids, SecY & the leader and mature domains of precursor proteins
10. Selection for loss of tetracycline resistance by Escherichia coli
11. Roles of SecG in ATP- and SecA-dependent protein translocation
12. SecY and SecA interact to allow SecA insertion & protein translocation across the Escherichia coli plasma membrane
13. Syd, a SecY-interacting protein, excludes SecA from the SecYE complex with altered SecY24 subunit
14. Two distinct ATP-binding domains are needed to promote protein export by Escherichia coli SecA ATPase
15. Two independent mechanisms down-regulate the intrinsic SecA ATPase activity
16. Characterization of a mutant form of SecA that alleviates a SecY defect at low temperature and shows a synthetic defect with the SecY alteration at high temperature
17. Distruption of the gene encoding p12 (SecG) reveals the direct involvement and important function of SecG in the protein translocation of Escherichia coli at low temperature
18. Inversion of the membrane topology of SecG coupled with SecA-dependent protein translocation
19. Regulation of membrane component required for protein secretion in Escherichia coli
20. Azide-resistant mutants of Escherichia coli alter the SecA protein, an azide-sensitive component of the protein export machinery
21. The Cs sec mutants of Escherichia coli reflect the cold sensitivity of protein export itself
22. Targeting of signal sequenceless protein for export in Escherichia coli with altered protein translocase
23. Protein transport across the eukaryotic endoplasmic reticulum and bacterial inner membranes
24. A mutation affecting the regulation of a secA-lacZ fusion defines a new sec gene
25. One of three transmembrane stretches is sufficient for the functioning of the SecE protein, a membrane component of the E. coli secretion machinery
26. Nucleotide sequence of the secA gene and secA (Ts) mutations preventing protein export in Escherichia coli
27. Stability and solvent accessibility of SecA protein of Escherichia coli
28. Coupled structure change of SecA and SecG revealed by the synthetic lethality of the secAcsR11 and ΔsecG, kan double mutant
29. Genetic analysis of SecY: Additional export-defective mutations and factors affecting their phenotypes
30. Protein translocation functions of Escherichia coli SecY: In vitro characterization of cold-sensitive secY mutants
31. Escherichia coli preprotein translocase