Targeted deletion of integrin-linked kinase reveals a role in T-cell chemotaxis and survival

Molecular and Cellular Biology

Volumn 25, Issue 24, 2005, Pages 11145-11155

  Liu, Emerson ^a,b   Sinha, Sumita ^a,b   Williams, Christine ^a,b   Cyrille, Marcoli ^a,b   Heller, Eric ^a,b   Snapper, Scott B ^a   Georgopoulos, Katia ^a   St Arnaud, Rene ^c   Force, Thomas ^d   Dedhar, Shoukat ^e   Gerszten, Robert E ^a,b  

^a MASSACHUSETTS GENERAL HOSPITAL   (United States)

^b HARVARD MEDICAL SCHOOL   (United States)

^c MCGILL UNIVERSITY   (Canada)

^d TUFTS UNIVERSITY SCHOOL OF MEDICINE   (United States)

^e UNIVERSITY OF BRITISH COLUMBIA   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

CRE RECOMBINASE; INTEGRIN LINKED KINASE; MACROPHAGE INFLAMMATORY PROTEIN 3BETA; PROTEIN KINASE B; RAC1 PROTEIN; SERINE; STROMAL CELL DERIVED FACTOR 1; YELLOW FLUORESCENT PROTEIN;

ANIMAL CELL; ANIMAL EXPERIMENT; APOPTOSIS; ARTICLE; CELL DEATH; CELL SURVIVAL; CHEMOTAXIS; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME PHOSPHORYLATION; ENZYME SUBSTRATE COMPLEX; EXTRACELLULAR MATRIX; GENE DELETION; HOMEOSTASIS; KNOCKOUT MOUSE; LYMPH NODE; MOUSE; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN FUNCTION; QUANTITATIVE ASSAY; SIGNAL TRANSDUCTION; SPLEEN; STRESS; T LYMPHOCYTE; THYMOCYTE;

ANIMALS; APOPTOSIS; CHEMOKINES, CC; CHEMOKINES, CXC; CHEMOTAXIS, LEUKOCYTE; GENE DELETION; GENE TARGETING; MICE; MICE, KNOCKOUT; PHOSPHORYLATION; PROTEIN-SERINE-THREONINE KINASES; PROTO-ONCOGENE PROTEINS C-AKT; SERINE; SIGNAL TRANSDUCTION; T-LYMPHOCYTES;

ANIMALIA; MUS MUSCULUS;

EID: 28544448740     PISSN: 02707306     EISSN: None     Source Type: Journal    
DOI: 10.1128/MCB.25.24.11145-11155.2005     Document Type: Article

References (32)

1. Delcommenne, M., C. Tan, V. Gray, L. Rue, J. Woodgett, and S. Dedhar. 1998. Phosphoinositide-3-OH kinase-dependent regulation of glycogen synthase kinase 3 and protein kinase B/AKT by the integrin-linked kinase. Proc. Natl. Acad. Sci. USA 95:11211-11216.
2. Friedrich, E. B., E. Liu, S. Sinha, S. Cook, D. S. Milstone, C. A. MacRae, M. Mariotti, P. J. Kuhlencordt, T. Force, A. Rosenzweig, R. St.-Arnaud, S. Dedhar, and R E. Gerszten. 2004. Integrin-linked kinase regulates endothelial cell survival and vascular development. Mol. Cell. Biol. 24:8134-8144.
3. Friedrich, E. B., S. Sinha, L. Li, S. Dedhar, T. Force, A. Rosenzweig, and R. E. Gerszten. 2002. Role of integrin-linked kinase in leukocyte recruitment. J. Biol. Chem. 277:16371-16375.
4. Fukui, Y., O. Hashimoto, T. Sanui, T. Oono, H. Koga, M. Abe, A. Inayoshi, M. Noda, M. Oike, T. Shirai, and T. Sasazuki. 2001. Haematopoietic cell-specific CDM family protein DOCK2 is essential for lymphocyte migration. Nature 412:826-831.
5. Funaraoto, S., R. Meili, S. Lee, L. Parry, and R. A. Firtel. 2002. Spatial and temporal regulation of 3-phosphoinositides by PI 3-kinase and PTEN mediates chemotaxis. Cell 109:611-623.
6. Grashoff, C., A. Aszodi, T. Sakai, E. B. Hunziker, and R. Fassler. 2003. Integrin-linked kinase regulates chondrocyte shape and proliferation. EMBO Rep. 4:432-138.
7. Gu, H., J. D. Marth, P. C, Orban, H, Mossmann, and K. Rajewsky, 1994. Deletion of a DNA polymerase beta gene segment in T cells using cell type-specific gene targeting. Science 265:103-106.
8. Gumienny, T. L., E. Brugnera, A. C. Tosello-Trampont, J. M. Kinchen, L. B. Haney, K. Nishiwaki, S. F. Walk, M. E. Nemergut, I. G. Macara, R. Francis, T. Schedl, Y. Qin, L. Van Aelst, M. O. Hengartner, and K. S. Ravichandran. 2001. CED-12/ELMO, a novel member of the CrkII/Dock180/Rac pathway, is required for phagocytosis and cell migration. Cell 107:27-41.
9. Hannigan, G., A. A. Troussard, and S. Dedhar. 2004. Integrin linked kinase: A cancer therapeutic target unique among its ILK. Nat. Rev. Cancer 5:51-63.
10. Hannigan, G. E., C. Leung-Hagesteijn, L. Fitz-Gibbon, M. G. Coppolino, G. Radeva, J. Filmus, J. C. Bell, and S. Dedhar. 1996. Regulation of cell adhesion and anchorage-dependent growth by a new beta 1-integrin-linked protein kinase. Nature 379:91-96.
11. Hirsch, E., V. L Katanaev, C. Garlanda, O. Azzolino, L. Pirola, L. Silengo, S. Sozzani, A. Mantovani, F. Altruda, and M. P. Wymann. 2000. Central role for G protein-coupled phosphoinositide 3-kinase gamma in inflammation. Science 287:1049-1053.
12. Lee, P. P., D. R. Fitzpatrick, C. Beard, H. K. Jessup, S. Lehar, K. W. Makar, M. Perez-Melgosa, M. T. Sweetser, M. S. Schüssel, S. Nguyen, S. R. Cherry, J. H. Tsai, S. M. Tucker, W. M. Weaver, A. Kelso, R. Jaenisch, and C. B. Wilson. 2001. A critical role for Dnmt1 and DNA methylation in T cell development, function, and survival. Immunity 15:763-774.
13. Lewandoski, M. 2001. Conditional control of gene expression in the mouse. Nat. Rev. Genet. 2:743-755.
14. Li, Z., H. Jiang, W. Xie, Z. Zhang, A. V. Smrcka, and D. Wu. 2000. Roles of PLC-beta2 and -beta3 and PI3Kgamma in chemoattractant-mediated signal transduction. Science 287:1046-1049.
15. Luster, A. D. 1998. Chemokines-chemotactic cytokines that mediate inflammation. N. Engl. J. Med. 338:436-445.
16. Mackinnon, A. C., H. Qadota, K. R. Norman, D. G. Moerman, and B. D. Williams. 2002. C. elegans PAT-4/ILK functions as an adaptor protein within integrin adhesion complexes. Curr. Biol. 12:787-797.
17. Matsui, T., L. Li, F. del Monte, Y. Fukui, T. Franke, R. Hajjar, and A. Rosenzweig. 1999. Adenoviral gene transfer of activated PI 3-kinase and Akt inhibits apoptosis of hypoxic cardiomyocytes in vitro. Circulation 100:2373-2379.
18. Nikolopoulos, S. N., and C. E. Turner. 2000. Actopaxin, a new focal adhesion protein that binds paxillin LD motifs and actin and regulates cell adhesion. J. Cell Biol. 151:1435-1448.
19. Peirson, S. N., J. N. Butler, and R. G. Foster. 2003. Experimental validation of novel and conventional approaches to quantitative real-time PCR data analysis. Nucleic Acids Res. 31:e73.
20. Rosenberger, G., I. Jantke, A. Gal, and K. Kutsche. 2003. Interaction of alphaPIX (ARHGEF6) with beta-parvin (PARVB) suggests an involvement of alphaPIX in integrin-mediated signaling. Hum. Mol. Genet. 12:155-167.
21. Sakai, T., S. Li, D. Docheva, C. Grashoff, K. Sakai, G. Kostka, A, Braun, A. Pfeifer, P. D. Yurchenco, and R. Fassler. 2003. Integrin-linked kinase (ILK) is required for polarizing the epiblast, cell adhesion, and controlling actin accumulation. Genes Dev. 17:926-940.
22. Sasaki, T., J. Irie-Sasaki, R. G. Jones, A. J. Oliveira-dos-Santos, W. L. Stanford, B. Bolon, A. Wakeham, A. Itie, D. Bouchard, I. Kozieradzki, N. Joza, T. W. Mak, P. S. Ohashi, A. Suzuki, and J. M. Penninger. 2000. Function of PI2Kgamma in thymocyte development, T cell activation, and neutrophil migration. Science 287:1040-1046.
23. Servant, G., O. D. Weiner, P. Herzmark, T. Balla, J. W. Sedat, and H. R. Bourne. 2000. Polarization of chemoattractant receptor signaling during neutrophil chemotaxis. Science 287:1037-1040.
24. Srinivas, S., T. Watanabe, C. S. Lin, C. M. William, Y. Tanabe, T. M. Jessell, and F. Costantini. 2001. Cre reporter strains produced by targeted insertion of EYFP and ECFP into the ROSA26 locus. BMC Dev. Biol. 1:4.
25. Takesono, A., R. Horai, M. Mandai, D. Dombroski, and P. L. Schwartzberg. 2004. Requirement for Tec kinases in chemokine-induced migration and activation of Cdc42 and Rac. Curr. Biol. 14:917-922.
26. Terpstra, L., J. Prud'homme, A. Arabian, S. Takeda, G. Karsenty, S. Dedhar, and R. St.-Arnaud. 2003. Reduced chondrocyte proliferation and chondrodysplasia in mice lacking the integrin-linked kinase in chondrocytes. J. Cell Biol. 162:139-148.
27. Troussard, A. A., N. M. Mawji, C. Ong, A. Mui, R. St.-Arnaud, and S. Dedhar. 2003. Conditional knock-out of integrin-linked kinase demonstrates an essential role in protein kinase B/Akt activation. J. Biol. Chem. 278: 22374-22378.
28. Tu, Y., F. Li, S. Goicoechea, and C. Wu. 1999. The LlM-only protein PINCH directly interacts with integrin-linked kinase and is recruited to integrin-rich sites in spreading cells. Mol. Cell. Biol. 19:2425-2434.
29. Turner, L., S. G. Ward, and J. Westwick. 1995. RANTES-activated human T lymphocytes. A role for phosphoinositide 3-kinase. J. Immunol. 155:2437-2444.
30. Wu, C. 1999. Integrin-linked kinase and PINCH: partners in regulation of cell-extracellular matrix interaction and signal transduction. J. Cell Sci. 112: 4485-4489.
31. Zervas, C. G., S. L. Gregory, and N. H. Brown. 2001. Drosophila integrin-linked kinase is required at sites of integrin adhesion to link the cytoskeleton to the plasma membrane. J. Cell Biol. 152:1007-1018.
32. Zhang, Y., K. Chen, Y. Tu, and C. Wu. 2004. Distinct roles of two structurally closely related focal adhesion proteins, alpha-parvins and beta-parvins, in regulation of cell morphology and survival. J. Biol. Chem. 279:41695-41705.