Actopaxin, a new focal adhesion protein that binds paxillin LD motifs and actin and regulates cell adhesion

Journal of Cell Biology

Volumn 151, Issue 7, 2000, Pages 1435-1447

  Nikolopoulos, Sotiris N ^a   Turner, Christopher E ^a  

^a State University of New York Upstate Medical University: College of Medicine   (United States)

Author keywords

Actin binding protein; CH domains; Focal adhesions; Hic 5; Paxillin

Indexed keywords

ACTIN; ACTIN BINDING PROTEIN; BINDING PROTEIN; CALPONIN; CELL ADHESION MOLECULE; F ACTIN; FOCAL ADHESION KINASE; PAXILLIN;

AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; CELL ADHESION; CELL MOTILITY; CELLULAR DISTRIBUTION; CYTOSKELETON; HUMAN; HUMAN CELL; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; RAT; SEQUENCE HOMOLOGY; SIGNAL TRANSDUCTION; WOUND HEALING;

ACTININ; ACTINS; AMINO ACID MOTIFS; AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; BLOTTING, WESTERN; CELL ADHESION; CELL LINE; CELL MOVEMENT; CLONING, MOLECULAR; CYTOSKELETAL PROTEINS; DNA-BINDING PROTEINS; FLUORESCENT ANTIBODY TECHNIQUE; FOCAL ADHESIONS; HELA CELLS; HUMANS; INTRACELLULAR SIGNALING PEPTIDES AND PROTEINS; MICROFILAMENT PROTEINS; MOLECULAR SEQUENCE DATA; MUTATION; PAXILLIN; PHOSPHOPROTEINS; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; PROTEIN TRANSPORT; RATS; RECOMBINANT FUSION PROTEINS; RNA, MESSENGER; SEQUENCE ALIGNMENT; SUBSTRATE SPECIFICITY; WOUND HEALING;

EID: 0034739856     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.151.7.1435     Document Type: Article

References (56)

1. PAK to the future
2. A novel regulator of p21-activated kinases
3. Structural comparisons of calponin homology domains: Implications for actin binding
4. Identification of LIM3 as the principal determinant of paxillin focal adhesion localization and characterization of a novel motif on paxillin directing vinculin and focal adhesion kinase binding
5. Paxillin LD motifs may define a new family of protein recognition domains
6. Serine and threonine phosphorylation of the paxillin LIM domains regulates paxillin focal adhesion localization and cell adhesion to fibronectin
7. Focal adhesions, contractility, and signaling
8. Crystal structure of a calponin homology domain
9. Does Vav bind to F-actin through a CH domain?
10. Integrins and signal transduction pathways: The road taken
11. Integrin-mediated signals regulated by members of the rho family of GTPases
12. Multiple sequence alignment with hierarchical clustering
13. Integrating the actin and vimentin cytoskeletons: Adhesion-dependent formation of fimbrin-vimentin complexes in macrophages
14. Intact LIM 3 and LIM 4 domains of paxillin are required for the association to a novel polyproline region (Pro 2) of protein-tyrosine phosphatase-PEST
15. Assembly of focal adhesions: Progress, paradigms, and portents
16. p21-activated protein kinase: A crucial component of morphological signaling?
17. p95-APP1 links membrane transport to Rac-mediated reorganization of actin
18. Regulators and effectors of the ARF GTPases
19. ARF: A key regulatory switch in membrane traffic and organelle structure
20. Remodeling of the actin cytoskeleton is coordinately regulated by protein kinase C and the ADP-ribosylation factor nucleotide exchange factor ARNO
21. Integrin signaling
22. The structure of an actin-crosslinking domain from human fimbrin
23. Recruitment of the LIM protein hic-5 to focal contacts of human platelets
24. Rho GTPases and the actin cytoskeleton
25. An SH3 domain-containing GTPase-activating protein for Rho and Cdc42 associates with focal adhesion kinase
26. The molecular architecture of focal adhesions
27. The importance of being proline: The interaction of proline-rich motifs in signaling proteins with their cognate domains
28. A new paxillin-binding protein, PAG3/Papalpha/KIAA0400, bearing an ADP-ribosylation factor GTPase-activating protein activity, is involved in paxillin recruitment to focal adhesions and cell migration
29. Binding of paxillin to alpha4 integrins modifies integrin-dependent biological responses
30. Predicting coiled coils from protein sequences
31. PAK kinases are directly coupled to the PIX family of nucleotide exchange factors
32. Rho, rac, and cdc42 GTPases regulate the assembly of multimolecular focal complexes associated with actin stress fibers, lamellipodia, and filopodia
33. ARF1 mediates paxillin recruitment to focal adhesions and potentiates Rho-stimulated stress fiber formation in intact and permeabilized Swiss 3T3 fibroblasts
34. The GIT family of ADP-ribosylation factor GTPase-activating proteins. Functional diversity of GIT2 through alternative splicing
35. Activation of Rac and Cdc42 by integrins mediates cell spreading
36. ADP-ribosylation factor 6 regulates a novel plasma membrane recycling pathway
37. Phosphorylation of the Src substrate Sam68 by Cdc2 during mitosis
38. Suppression of tumorigenicity in transformed cells after transfection with vinculin cDNA
39. Analysis of the binding of the Src homology 2 domain of Csk to tyrosine-phosphorylated proteins in the suppression and mitotic activation of c-Src
40. The related adhesion focal tyrosine kinase forms a complex with paxillin in hematopoietic cells
41. pp125FAK-dependent tyrosine phosphorylation of paxillin creates a high affinity binding site for Crk
42. Direct association of protein-tyrosine phosphatase PTP-PEST with paxillin
43. Localization of endogenous ARF6 to sites of cortical actin rearrangement and involvement of ARF6 in cell spreading
44. CH domains revisited
45. Direct association of pp125FAK with paxillin, the focal adhesion-targeting mechanism of pp125FAK
46. Talin at myotendinous junctions
47. The bovine papillomavirus E6 protein binds to the LD motif repeats of paxillin and blocks its interaction with vinculin and the focal adhesion kinase
48. Paxillin
49. Transmembrane molecular assemblies in cell-extracellular matrix interactions
50. Primary sequence of paxillin contains putative SH2 and SH3 domain binding motifs and multiple LIM domains: Identification of a vinculin and pp125Fak-binding region
51. Paxillin: A new vinculin-binding protein present in focal adhesions
52. Localization of paxillin, a focal adhesion protein, to smooth muscle dense plaques, and the myotendinous and neuromuscular junctions of skeletal muscle
53. Paxillin LD4 motif binds PAK and PIX through a novel 95-kD ankyrin repeat, ARF-GAP protein: A role in cytoskeletal remodeling
54. Rho GTPases and signaling networks
55. Association of bovine papillomavirus type 1 E6 oncoprotein with the focal adhesion protein paxillin through a conserved protein interaction motif
56. Characterisation of the paxillin-binding site and the C-terminal focal adhesion targeting sequence in vinculin