Agrobacterium-mediated transformation of Botrytis cinerea, simple purification of monokaryotic transformants and rapid conidia-based identification of the transfer-DNA host genomic DNA flanking sequences

Current Genetics

Volumn 44, Issue 3, 2003, Pages 164-171

  Rolland, Stéphane ^a   Jobic, Cécile ^a   Fèvre, Michel ^a   Bruel, Christophe ^a  

^a UNIVERSITÉ DE LYON   (France)

Author keywords

Beta glucuronidase; Green fluorescent protein; Sclerotinia sclerotiorum

Indexed keywords

BETA GLUCURONIDASE; DNA; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; PROTEINASE;

ARTICLE; BOTRYTIS CINEREA; CELL COUNT; CONIDIUM; CONTROLLED STUDY; DNA FLANKING REGION; DNA INTEGRATION; DNA PURIFICATION; DNA SEQUENCE; DNA TRANSFER; ENZYME RELEASE; GENETIC CODE; GENETIC TRANSCRIPTION; GENETIC TRANSFORMATION; HOST; NONHUMAN; PLANT DISEASE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN BINDING; RHIZOBIUM RADIOBACTER; SOMATIC CELL GENETICS; TANDEM REPEAT;

ASCOMYCOTA; BLOTTING, SOUTHERN; BLOTTING, WESTERN; BOTRYTIS; DNA PRIMERS; DNA, BACTERIAL; GLUCURONIDASE; GREEN FLUORESCENT PROTEINS; LUMINESCENT PROTEINS; MICROSCOPY, FLUORESCENCE; PLASMIDS; POLYMERASE CHAIN REACTION; RHIZOBIUM RADIOBACTER; TRANSFORMATION, GENETIC;

AGROBACTERIUM; AGROBACTERIUM TUMEFACIENS; BOTRYOTINIA FUCKELIANA; BOTRYTIS; FUNGI; NEGIBACTERIA; SCLEROTINIA; SCLEROTINIA SCLEROTIORUM;

EID: 0344149573     PISSN: 01728083     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00294-003-0438-8     Document Type: Article

References (29)

1. Billon-Grand G, Poussereau N, Fèvre M (2002) The extracellular proteases secreted in vitro and in planta by the phytopathogenic fungus Sclerotinia sclerotiorum. J Phytopathol 150:507-511
2. Bradford M (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254
3. Bundock P, Hooykaas PJJ (1996) Integration of Agrobacterium tumefaciens T-DNA in the Saccharomyces cerevisiae genome by illegitimate recombination. Proc Natl Acad Sci USA 93:15272-15275
4. Bundock P, Dulk-Ras A den, Beijersbergen A, Hooykaas PJJ (1995) Trans-kingdom T-DNA transfer from Agrobacterium tumefaciens to Saccharomyces cerevisiae. EMBO J 14:3206-3214
5. Bundock P, Attikum H van, Dulk-Ras A den, Hooykaas PJJ (2002) Insertional mutagenesis in yeasts using T-DNA from Agrobacterium tumefaciens. Yeast 19:529-536
6. Chen X, Stone M, Schlagnhaufer C, Romaine CP (2000) A fruiting body tissue method for efficient Agrobacterium-mediated transformation of Agaricus bisporus. Appl Envir Microbiol 66:4510-4513
7. Combier J-P, Melayah D, Raffier C, Gay G, Marmeisse R (2003) Agrobacterium tumefaciens-mediated transformation as a tool for insertional mutagenesis in the symbiotic ectomycorrhizal fungus Heneloma cylindrosporum. FEMS Microbiol Lett 220:141-148
8. Ferreira AVB, Louise GN (1996) PCR from fungal spores after microwave treatment. Fungal Genet Newl 43:25-26
9. Fraissinet-Tachet L, Reymond-Cotton P, Fèvre M (1996) Molecular karyotype of the phytopathogenic fungus Sclerotinia sclerotiorum. Curr Genet 29:496-501
10. Fullner KJ, Nester EW (1996) Temperature affects the T-DNA transfer machinery of Agrobacterium tumefaciens. J Bacteriol 178:1498-1504
11. Gouka RJ, Gerk C, Hooykaas PJJ, Bundock P, Musters W, Verrips CT, Groot MJA de (1999) Transformation of Aspergillus awamori by Agrobacterium tumefaciens-mediated homologous recombination. Nat Biotechnol 17:598-601
12. Groot MJA de, Bundock P, Hooykaas PJJ, Beijersbergen AGM (1998) Agrobacterium tumefaciens-mediated transformation of filamentous fungi. Nat Biotechnol 16:839-842
13. Hamada W, Reignault P, Bompeix G, Boccara M (1994) Transformation of B. cinerea with the hygromycin B resistance gene, hph. Curr Genet 26:251-255
14. Hanif M, Pardo AG, Gorfer M, Raudaskoski M (2002) T-DNA transfer and integration in the ectomycorrhizal fungus Suillus bovinus using hygromycin B as a selectable marker. Curr Genet 41:183-188
15. Kunik T, Tzfira T, Kapulnik Y, Gafni Y, Dingwall C, Citovsky V (2001) Genetic transformation of Hela cells by Agrobacterium. Proc Natl Acad Sci USA 98:1871-1876
16. Liu Y-G, Mitsukawa N, Oosumi T, Whittier RF (1995) Efficient isolation and mapping of Arabidopsis thaliana T-DNA insert junctions by thermal asymmetric interlaced PCR. Plant J 8:457-463
17. Lorang JM, Tuori RP, Martinez JP, Sawyer TL, Redman RS, Rollins JA, Wolpert TJ, Johnson KB, Rodriguez RJ, Dickman MB, Ciuffetti LM (2001) Green fluorescent protein is lighting up fungal biology. Appl Environ Microbiol 67:1987-1994
18. Martineau B, Voelker TA, Sanders RA (1994) On defining T-DNA. Plant Cell 6:1032-1033
19. Mikosch TSP, Lavrijssen B, Sonnenberg ASM, Griensven LJLD van (2001) Transformation of the cultivated mushroom Agaricus bisporus (Lange) using T-DNA from Agrobacterium tumefaciens. Curr Genet 39:35-39
20. Mullins ED, Chen X, Romaine P, Raina R, Geiser DM, Kang S (2001) Agrobacterium-mediated transformation of Fusarium oxysporum: an efficient tool for insertional mutagenesis and gene transfer. Phytopathology 91:173-180
21. Poussereau N, Creton S, Billon-Grand G, Rascle C, Fèvre M (2001) Regulation of acp1, encoding a non-aspartyl acid protease expressed during pathogenesis of Sclerotinia sclerotiorum. Microbiology 147:717-726
22. Purdy LH (1979) Sclerotinia sclerotiorum: history, diseases and symptomology, host range, geographic distribution and impact. Phytopathology 69:875-880
23. Raeder U, Broda P (1985) Rapid preparation of DNA from filamentous fungi. Lett Appl Microbiol 1:17-20
24. Rech P, Grima-Pettenati J, Jauneau A (2003) Fluorescence microscopy: a powerful technique to detect low GUS activity in vascular tissues. Plant J 33:205-209
25. Rha E, Park HJ, Kim MO, Chung YR, Lee CW, Kim JW (2001) Expression of exo-polygalacturonases in Botrytis cinerea. FEMS Microbiol Lett 201:105-109
26. Southern E (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503-517
27. Urbanek H, Kaczmarek A (1985) Extracellular proteinases of the isolate of Botrytis cinerea virulent to apple tissues. Acta Biochim Pol 32:101-109
28. Walton JD (1994) Deconstructing the cell wall. Plant Physiol 104:1113-1118
29. Zhang A, Lu P, Dahl-Roshak AM, Paress PS, Kennedy S, Tkacz JS, An Z (2003) Efficient disruption of a polyketide synthase gene (psk1) required for melanin synthesis through Agrobacterium-mediated transformation of Glarea lozoyensis. Mol Gen Genomics 268:645-655