Negative selection using thymidine kinase increases the efficiency of recovery of transformants with targeted genes in the filamentous fungus Leptosphaeria maculans

Current Genetics

Volumn 45, Issue 4, 2004, Pages 249-255

  Gardiner, Donald M ^a   Howlett, Barbara J ^a  

^a UNIVERSITY OF MELBOURNE   (Australia)

Author keywords

Homologous recombination; Negative selection; Toxic gene

Indexed keywords

ABC TRANSPORTER; FLOXURIDINE; FUNGAL DNA; GANCICLOVIR; NUCLEOSIDE ANALOG; PROTEIN HISTIDINE KINASE; THYMIDINE DERIVATIVE; THYMIDINE KINASE; TRIFLURIDINE;

ARTICLE; ASCOMYCETES; ASPERGILLUS NIDULANS; CELL KILLING; CONTROLLED STUDY; CYTOTOXICITY; GENE CONSTRUCT; GENE DISRUPTION; GENE EXPRESSION REGULATION; GENE MUTATION; GENE TARGETING; GENE VECTOR; GENETIC SCREENING; GENETIC SELECTION; GENETIC TRANSFORMATION; GROWTH INHIBITION; HERPES SIMPLEX VIRUS; LEPTOSPHAERIA MACULANS; MARKER GENE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; RHIZOBIUM RADIOBACTER;

ATP-BINDING CASSETTE TRANSPORTERS; FUNGI; PROTEIN KINASES; RHIZOBIUM RADIOBACTER; THYMIDINE KINASE; TRANSFECTION;

AGROBACTERIUM; AGROBACTERIUM TUMEFACIENS; ASPERGILLUS; EMERICELLA NIDULANS; FUNGI; LEPTOSPHAERIA; LEPTOSPHAERIA MACULANS; SIMPLEXVIRUS;

EID: 2542425574     PISSN: 01728083     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00294-004-0488-6     Document Type: Article

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