Identification and purification of a soluble region of BubR1: A critical component of the mitotic checkpoint complex

Protein Expression and Purification

Volumn 44, Issue 1, 2005, Pages 1-9

  Yoon, Jongchul ^a   Kang, Yup ^b   Kim, Kyunggon ^a   Park, Jungeun ^a   Kim, Youngsoo ^a  

^a Seoul National University   (South Korea)

^b Ajou University School of Medicine   (South Korea)

Author keywords

Anaphase promoting complex; BubR1; Structure

Indexed keywords

BUB1 SPINDLE CHECKPOINT PROTEIN; CDC20 PROTEIN, HUMAN; CELL CYCLE PROTEIN; PROTEIN KINASE;

AMINO ACID SEQUENCE; ANAPHASE; ANIMAL; ARTICLE; CHROMOSOME SEGREGATION; GENE VECTOR; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MOLECULAR CLONING; MOLECULAR GENETICS; PHYSIOLOGY; PROTEIN BINDING; PROTEIN MOTIF; PROTEIN TERTIARY STRUCTURE; RAT; SOLUBILITY;

AMINO ACID MOTIFS; AMINO ACID SEQUENCE; ANAPHASE; ANIMALS; CELL CYCLE PROTEINS; CHROMOSOME SEGREGATION; CLONING, MOLECULAR; GENETIC VECTORS; MOLECULAR SEQUENCE DATA; PROTEIN BINDING; PROTEIN KINASES; PROTEIN STRUCTURE, TERTIARY; RATS; SOLUBILITY;

ESCHERICHIA COLI;

EID: 26044476321     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2005.04.020     Document Type: Article

References (38)

1. C.L. Rieder, R.W. Cole, A. Khodjakov, and G. Sluder The checkpoint delaying anaphase in response to chromosome mono-orientation is mediated by unattached kinetochores J. Cell Biol. 130 1995 941 948
2. R.H. Chen, A. Shevchenko, M. Mann, and A.W. Murray Spindle checkpoint protein Xmad1 recruits Xmad2 to unattached kinetochores J. Cell Biol. 143 1998 283 295
3. Y. Li, and R. Benezra Identification of a human mitotic checkpoint gene:hsMAD2 Science 274 1996 246 248
4. S.S. Taylor, and F. McKeon Kinetochore localization of murine Bub1 is required for normal mitotic timing and checkpoint response to spindle damage Cell 89 1997 727 735
5. S.S. Taylor, E. Ha, and F. McKeon The human homologue of Bub3 is required for kinetochore localization of Bub1 and a Mad3/Bub1-related protein kinase J. Cell Biol. 142 1998 1 11
6. G.K. Chan, B.T. Schaar, and T.J. Yen Characterization of the kinetochore binding domain of CENP-E reveals interactions with the kinetochore proteins CENP-F and hBUBR1 J. Cell Biol. 143 1998 49 63
7. G.K. Chan, S.A. Jablonski, V. Sudakin, J.C. Hittle, and T.J. Yen Human BUBR1 is a mitotic checkpoint kinase that monitors CENP-E functions at kinetochores and binds the cyclosome/APC J. Cell Biol. 146 1999 941 954
8. R.H. Chen, J.C. Waters, E.D. Salmon, and A.W. Murray Association of spindle assembly checkpoint component XMAD2 with unattached kinetochores Science 274 1996 242 246
9. J.V. Shah, and D.W. Cleveland Waiting for anaphase: Mad2 and the spindle assembly checkpoint Cell 103 2000 997 1000
10. R.W. King, J.-M. Peters, S. Tugendreich, M. Rolfe, P. Hieter, and M.W. Kirschner A 20S complex containing CDC27 and CDC16 catalyses the mitosis-specific conjugation of ubiquitin to cyclin B Cell 81 1995 279 288
11. R.W. King, R.J. Deshaies, J.M. Peters, and M.W. Kirschner How proteolysis drives the cell cycle Science 274 1996 1652 1659
12. V. Sudakin, D. Ganoth, A. Dahan, H. Heller, J. Hershko, F.C. Luca, J.V. Ruderman, and A. Hershko The cyclosome, a large complex containing cyclin-selective ubiquitin ligase activity, targets cyclins for destruction at the end of mitosis Mol. Biol. Cell 6 1995 185 197
13. A. Hershko, and A. Ciechanover The ubiquitin system Annu. Rev. Biochem. 67 1998 425 479
14. V. Sudakin, G.K. Chan, and T.J. Yen Checkpoint inhibition of the APC/C in HeLa cells is mediated by a complex of BUBR1, BUB3, CDC20, and MAD2 J. Cell Biol. 154 2001 925 936
15. Z. Tang, R. Bharadwaj, B. Li, and H. Yu Mad2-independent inhibition of APC Cdc20 by the mitotic checkpoint protein BubR1 Dev. Cell 1 2001 227 237
16. R. Bharadwaj, and H. Yu The spindle checkpoint, aneuploidy, and cancer Oncogene 23 2004 2016 2057
17. Y. Mao, A. Abrieu, and D.W. Cleveland Activating and silencing the mitotic checkpoint through CENP-E-dependent activation/inactivation of BubR1 Cell 114 2003 87 98
18. G. Fang Checkpoint protein BubR1 acts synergistically with Mad2 to inhibit anaphase-promoting complex Mol. Biol. Cell 13 2002 755 766
19. B.T. Schaar, G.K. Chan, P. Maddox, E.D. Salmon, and T.J. Yen CENP-E function at kinetochores is essential for chromosome alignment J. Cell Biol. 139 1997 1373 1382
20. K.W. Wood, R. Sakowicz, L.S. Goldstein, and D.W. Cleveland CENP-E is a plus end-directed kinetochore motor required for metaphase chromosome alignment Cell 91 1997 357 366
21. X. Yao, A. Abrieu, Y. Zheng, K.F. Sullivan, and D.W. Cleveland CENP-E forms a link between attachment of spindle microtubules to kinetochores and the mitotic checkpoint Nat. Cell Biol. 2 2000 484 491
22. X. Luo, G. Fang, M. Coldiron, Y. Lin, H. Yu, M.W. Kirschner, and G. Wagner Structure of the Mad2 spindle checkpoint protein and its interaction with Cdc20 Nat. Struct. Biol. 7 2000 224 229
23. L. Sironi, M. Mapelli, S. Knapp, A.D. Antoni, K.T. Jeang, and A. Musacchio Crystal structure of the tetrameric Mad1-Mad2 core complex: implication of a 'safety belt' binding mechanism for the spindle checkpoint EMBO J. 21 2002 2496 2506
24. N.A. Larsen, and S.C. Harrison Crystal structure of the spindle assembly checkpoint protein Bub3 J. Mol. Biol. 344 2004 885 892
25. C. Gustafsson, S. Govindarajan, and J. Minshull Codon bias and heterogous protein expression Trends Biotechnol. 22 2004 346 353
26. S.F. Altschul, T.L. Madden, A.A. Schaffe, J. Zhang, Z. Zhang, W. Miller, and D.J. Lipman Gapped BLAST and PSI-BLAST: a new generation of protein database search programs Nucleic Acids Res. 1 1997 3389 3402
27. J. Garnier, J.F. Gibrat, and B. Robson GOR secondary structure prediction method version IV Methods Enzymol. 266 1996 540 553
28. Y. Guermeur, Combinaison de classifieurs statistiques, Application a la prediction de structure secondaire des proteines, Ph.D. Thesis.
29. B. Rost, and C. Sander Prediction of protein secondary structure at better than 70% accuracy J. Mol. Biol. 232 1993 584 599
30. D. Frishman, and P. Argos Incorporation of non-local interactions in protein secondary structure prediction from the amino acid sequence Protein Eng. 9 1996 133 142
31. J.M. Levin, B. Robson, and J. Garnier An algorithm for secondary structure determination in proteins based on sequence similarity FEBS Lett. 205 1986 303 308
32. W.C. Johnson Jr. Protein secondary structure and circular dichroism: a practical guide Proteins: structure, function, and genetics 7 1990 205 214
33. L. Whitmore, and B.A. Wallace DICHROWEB: an online server for protein secondary structure analyses from circular dichroism spectroscopic data Nucleic Acids Res. 32 2004 668 673
34. J. Rosenfeld, J. Capdevielle, J.C. Guillemot, and P. Ferrara In-gel digestion of proteins for internal sequence analysis after one- or two-dimensional gel electrophoresis Anal. Biochem. 203 1992 173 179
35. M. Shevchenko, M. Wilm, O. Vorm, and M. Mann Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels Anal. Chem. 68 1996 850 858
36. C. Scheler, S. Lamer, Z. Pan, X.P. Li, J. Salnikow, and P. Jungblut Peptide mass fingerprint sequence coverage from differently stained proteins on two-dimensional electrophoresis patterns by matrix assisted laser desorption/ionization-mass spectrometry (MALDI-MS) Electrophoresis 19 1998 918 927
37. S.W. Provencher, and J. Glockner Estimation of globular protein secondary structure from circular dichroism Biochemistry 20 1981 33 37
38. I.H.M. Van Stokkum, H.J.W. Spoelder, M. Bloemendal, R. Van Grondelle, and F.C.A. Groen Estimation of protein secondary structure and error analysis from CD spectra Anal. Biochem. 191 1990 110 118