Structure of the Mad2 spindle assembly checkpoint protein and its interaction with Cdc20

Nature Structural Biology

Volumn 7, Issue 3, 2000, Pages 224-229

  Lou, Xuelian ^a,b   Fang, Guowei ^a   Coldiron, Melissa ^b   Lin, Yingxi ^a   Yu, Hongtao ^b   Kirschner, Marc W ^a   Wagner, Gerhard ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

^b UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; CELL CYCLE PROTEIN 20; M PROTEIN; PROTEIN MAD2; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; DELETION MUTANT; MITOSIS SPINDLE; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN INTERACTION; PROTEIN STRUCTURE;

AMINO ACID SEQUENCE; BINDING SITES; CALCIUM-BINDING PROTEINS; CARRIER PROTEINS; CELL CYCLE PROTEINS; DIMERIZATION; FUNGAL PROTEINS; HUMANS; MITOTIC SPINDLE APPARATUS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; NUCLEAR PROTEINS; PROTEIN FOLDING; PROTEIN STRUCTURE, QUATERNARY; PROTEIN STRUCTURE, SECONDARY; REPRESSOR PROTEINS; SACCHAROMYCES CEREVISIAE PROTEINS; SEQUENCE ALIGNMENT; SEQUENCE DELETION; SOLUTIONS; STRUCTURE-ACTIVITY RELATIONSHIP;

EID: 0343986407     PISSN: 10728368     EISSN: None     Source Type: Journal    
DOI: 10.1038/73338     Document Type: Article

References (38)

1. Nicklas, R.B., Ward, S.C. & Gorbsky, G.J. Kinetochore chemistry is sensitive to tension and may link mitotic forces to a cell cycle checkpoint. J. Cell. Biol. 130, 929-939 (1995).
2. Murray, A.W. The genetics of cell cycle checkpoints. Curr. Opin. Genet. Dev. 5, 5-11 (1995).
3. Hardwick, K.G. The spindle checkpoint. Trends Genet. 14, 1-4 (1998).
4. Nicklas, R.B. How cells get the right chromosomes. Science 275, 632-637 (1997).
5. Nasmyth, K. Separating sister chromatids. Trends Biochem. Sci. 24, 98-104 (1999).
6. Hoyt, M.A., Totis, L. & Roberts, B.T. S. cerevisiae genes required for cell cycle arrest in response to loss of microtubule function. Cell 66, 507-517 (1991).
7. Li, R. & Murray, A.W. Feedback control of mitosis in budding yeast. Cell 66, 519-531 (1991).
8. Chen, R.H., Waters, J.C., Salmon, E.D. & Murray, A.W. Association of spindle assembly checkpoint component XMAD2 with unattached kinetochores. Science 274, 242-246 (1996).
9. Chen, R.H., Shevchenko, A., Mann, M. & Murray, A.W. Spindle checkpoint protein Xmad1 recruits Xmad2 to unattached kinetochores. J. Cell Biol. 143, 283-295 (1998).
10. Li, Y. & Benezra, R. Identification of a human mitotic checkpoint gene: hsMAD2. Science 274, 246-248 (1996).
11. Taylor, S.S. & McKeon, F. Kinetochore localization of murine Bub1 is required for normal mitotic timing and checkpoint response to spindle damage. Cell 89, 727-735 (1997).
12. Taylor, S.S., Ha, E. & McKeon, F. The human homologue of Bub3 is required for kinetochore localization of Bub1 and a Mad3/Bub1-related protein kinase. J. Cell Biol. 142, 1-11 (1998).
13. Jin, D.-Y., Spencer, F. & Jeang, K.-T. Human T cell leukemia virus type 1 oncoprotein Tax targets the human mitotic checkpoint protein MAD1. Cell 93, 81-91 (1998).
14. Farr, K.A. & Hoyt, M.A. Bub1p kinase activates the Saccharomyces cerevisiae spindle assembly checkpoint. Mol. Cell Biol. 18, 2738-2747 (1998).
15. Hardwick, K.G. & Murray, A.W. Mad1p, a phosphoprotein component of the spindle assembly checkpoint in budding yeast. J. Cell Biol. 131, 709-720 (1995).
16. Cuif, M.H. et al. Characterization of GAPCenA, a GTPase activating protein for rab6, part of which associates with the centrosome. EMBO J. 18, 1772-1782 (1999).
17. Alexandru, G., Zachariae, W., Schleiffer, A. & Nasmyth, K. Sister chromatid separation and chromosome re-duplication are regulated by different mechanisms in response to spindle damage. EMBO J. 18, 2707-2721 (1999).
18. Li, R. Bifurcation of the mitotic checkpoint pathway in budding yeast. Proc. Natl. Acad. Sci. U. S. A. 96, 4989-4994 (1999).
19. Fang, G., Yu, H. & Kirschner, M.W. Direct binding of CDC20 protein family members activates the anaphase-promoting complex in mitosis and G1. Mol. Cell 2, 163-171 (1998).
20. Kim, S.H., Lin, D.P., Matsumoto, S., Kitazono, A. & Matsumoto, T. Fission yeast Slp1: an effector of the Mad2-dependent spindle checkpoint. Science 279, 1045-1047 (1998).
21. Hwang, L.H. et al. Budding yeast Cdc20: a target of the spindle checkpoint. Science 279, 1041-1044 (1998).
22. Fang, G., Yu, H. & Kirschner, M.W. The checkpoint protein MAD2 and the mitotic regulator CDC20 form a ternary complex with the anaphase-promoting complex to control anaphase initiation. Genes Dev. 12, 1871-1883 (1998).
23. Clore, G.M. & Gronenborn, A.M. Determining the structures of large proteins and protein complexes by NMR. Trends Biotechnol. 16, 22-34 (1998).
24. Brünger, A.T. X-PLOR Version 3.1 (Yale University Press, New Haven, CT, 1993).
25. Holm, L. & Sander, C. Protein structure comparison by alignment of distance matrices. J. Mol. Biol. 233, 123-138 (1993).
26. Aravind, L. & Koonin, E.V. The HORMA domain: a common structural denominator in mitotic checkpoints, chromosome synapsis and DNA repair. Trends Biochem. Sci. 23, 284-286 (1998).
27. Chen, R.H., Brady, D.M., Smith, D., Murray, A.W. & Hardwick, K.G. The spindle checkpoint of budding yeast depends on a tight complex between the mad1 and mad2 proteins. Mol. Biol. Cell 10, 2607-2618 (1999).
28. 2H labeled proteins with high sensitivity. J. Am. Chem. Soc. 116, 11655-11666 (1994).
29. Matsuo, H., Li, H. & Wagner, G. A sensitive HN(CA)CO experiment for deuterated proteins. J. Magn. Reson. B 110, 112-115 (1996).
30. Matsuo, H., Kupce, E., Li, H. & Wagner, G. Increased sensitivity in HNCA and HN(CO)CA experiments by selective Cβ; decoupling. J. Magn. Reson. B 113, 91-96 (1996).
31. 2O samples of proteins. J. Magn. Reson. B 101, 333-337 (1993).
32. Matsuo, H., Kupce, E. & Wagner, G. Resolution and sensitivity gain in HCCH-TOCSY experiments by homonuclear Cβ decoupling. J. Magn. Reson. B 113, 190-194 (1996).
33. 13C magnetization. J. Magn. Reson. B101, 114-119 (1993).
34. Lyons, B.A. & Montelione, G.T. An HCCNH triple-resonance experiment using carbon-13 isotropic mixing for correlating backbone amide and side-chain aliphatic resonances in isotopically enriched proteins. J. Magn. Reson. B 101, 206-209 (1993).
35. Lin, Y. & Wagner, G. Efficient side chain and backbone assignments in large proteins. J. Biomol. NMR 15, 227-239 (1999).
36. 13C-labeling. J. Biomol. NMR 2, 323-334 (1992).
37. Koradi, R., Billeter, M. & Wüthrich, K. MOLMOL: a program for display and analysis of macromolecular structures. J. Mol. Graph. 14, 51-55 (1996).
38. Nicholls, A., Sharp, K.A. & Honig, B. Protein Folding and association: insights from the interfacial and thermodynamic properties of hydrocarbons. Proteins 11, 281-296 (1991).