Mutagenic mapping of helical structures in the transmembrane segments of the yeast α-factor receptor

Journal of Molecular Biology

Volumn 317, Issue 5, 2002, Pages 765-788

  Martin, Negin P ^a   Ćelić, Andjelka ^a   Dumont, Mark E ^a  

^a UNIVERSITY OF ROCHESTER SCHOOL OF MEDICINE AND DENTISTRY   (United States)

Author keywords

G protein coupled receptor; Membrane protein; Mutagenesis; STE2; factor receptor

Indexed keywords

AMINO ACID; G PROTEIN COUPLED RECEPTOR; MATING HORMONE ALPHA FACTOR RECEPTOR; MITOGEN ACTIVATED PROTEIN KINASE; PROLINE; RHODOPSIN; UNCLASSIFIED DRUG;

ALLELE; ALPHA HELIX; AMINO ACID SUBSTITUTION; ARTICLE; CONTROLLED STUDY; HYDROPHOBICITY; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN INTERACTION; PROTEIN STRUCTURE; YEAST;

EID: 0036298264     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2002.5444     Document Type: Article

References (44)

1. G protein-coupled receptors. I. Diversity of receptor-ligand interactions
2. Signal propagation and regulation in the mating pheromone response pathway of the yeast Saccharomyces cerevisiae
3. The pheromone response pathway in Saccharomyces cerevisiae
4. Golf complements a GPA1 null mutation in Saccharomyces cerevisiae and functionally couples to the STE2 pheromone receptor
5. Functional coupling of mammalian receptors to the yeast mating pathway using novel yeast/mammalian G protein alpha-subunit chimeras
6. Crystal structure of rhodopsin: A G protein-coupled receptor
7. Evidence for kinship between diverse G-protein coupled receptors
8. Genetic mapping of the human C5a receptor. Identification of transmembrane amino acids critical for receptor function
9. C5a receptor activation. Genetic identification of critical residues in four transmembrane helices
10. Role of Sst2 in modulating G protein-coupled receptor signaling
11. Dominant negative mutations in the alpha-factor receptor, a G protein-coupled receptor encoded by the STE2 gene of the yeast Saccharomyces cerevisiae
12. The C terminus of the S. cerevisiae alpha-pheromone receptor mediates an adaptive response to pheromone
13. Genetic interactions among the transmembrane segments of the G protein coupled receptor encoded by the yeast STE2 gene
14. Identification of a polar region in transmembrane domain 6 that regulates the function of the G protein-coupled alpha-factor receptor
15. Mechanisms governing the activation and trafficking of yeast G protein-coupled receptors
16. A limited spectrum of mutations causes constitutive activation of the yeast alpha-factor receptor
17. Interaction between transmembrane domains five and six of the alpha-factor receptor
18. Systematic mutagenesis of the yeast mating pheromone receptor third intracellular loop
19. Edg-2/Vzg-1 couples to the yeast pheromone response pathway selectively in response to lysophosphatidic acid
20. Control of yeast mating signal transduction by a mammalian beta 2-adrenergic receptor and Gs alpha subunit
21. Investigation of growth hormone releasing hormone receptor structure and activity using yeast expression technologies
22. Functional coupling of a mammalian somatostatin receptor to the yeast pheromone response pathway
23. Pharmacological characterization of the rat A2a adenosine receptor functionally coupled to the yeast pheromone response pathway
24. Hydrophobic parameters pi of amino-acid side-chains from the partitioning of N-acetyl-amino-acid amides
25. A model of evolutionary change in proteins
26. Mutational analysis of the role of N-glycosylation in alpha-factor receptor function
27. Cytoplasmic tail phosphorylation of the alpha-factor receptor is required for its ubiquitination and internalization
28. In vivo topological analysis of Ste2, a yeast plasma membrane protein, by using beta-lactamase gene fusions
29. An alpha-carbon template for the transmembrane helices in the rhodopsin family of G-protein-coupled receptors
30. Assembly of G protein-coupled receptors from fragments: Identification of functional receptors with discontinuities in each of the loops connecting transmembrane segments
31. Mutation of Pro-258 in transmembrane domain 6 constitutively activates the G protein-coupled alpha-factor receptor
32. Polar side-chains drive the association of model transmembrane peptides
33. Polar residues drive association of polyleucine transmembrane helices
34. Helix geometry in proteins
35. Amino acid distributions in integral membrane protein structures
36. Exploring the allowed sequence space of a membrane protein
37. A passive transmembrane helix
38. Transmembrane structure of an inwardly rectifying potassium channel
39. Functional domains of a peptide hormone receptor: The alpha-factor receptor (STE2 gene product) of the yeast Saccharomyces cerevisiae
40. The STE2 gene product is the ligand-binding component of the alpha-factor receptor of Saccharomyces cerevisiae
41. Agonist-specific conformational changes in the yeast alpha-factor pheromone receptor
42. Genetic approaches for studying the structure and function of G protein-coupled receptors in yeast
43. New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites
44. A simplified representation of protein conformations for rapid simulation of protein folding