Composition and functional characterization of the yeast spliceosomal penta-snRNP

Molecular Cell

Volumn 9, Issue 1, 2002, Pages 31-44

  Stevens, Scott W ^a   Ryan, Daniel E ^a   Ge, Helen Y ^b   Moore, Roger E ^b   Young, Mary K ^b   Lee, Terry D ^b   Abelson, John ^a  

^a CALIFORNIA INSTITUTE OF TECHNOLOGY   (United States)

^b CITY OF HOPE NATIONAL MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MESSENGER RNA PRECURSOR; SMALL NUCLEAR RIBONUCLEOPROTEIN;

ARTICLE; CONTROLLED STUDY; NONHUMAN; PROTEIN ANALYSIS; PROTEIN FUNCTION; PROTEIN PROTEIN INTERACTION; RNA SPLICING; SPLICEOSOME; YEAST;

EID: 0036158942     PISSN: 10972765     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1097-2765(02)00436-7     Document Type: Article

References (68)

1. The yeast PRP6 gene encodes a U4/U6 small nuclear ribonucleoprotein particle (snRNP) protein, and the PRP9 gene encodes a protein required for U2 snRNP binding
2. The yeast Mud2 protein - An interaction with Prp11 defines a bridge between commitment complexes and U2 snRNP addition
3. SLU7 and a novel activity, SSF1, act during the PRP16-dependent step of yeast pre-mRNA splicing
4. The Saccharomyces cerevisiae PRP21 gene product is an integral component of the prespliceosome
5. PRP4: A protein of the yeast U4/U6 small nuclear ribonucleoprotein particle
6. Genetic and physical interactions between factors involved in both cell cycle progression and pre-mRNA splicing in Saccharomyces cerevisiae
7. Protein components specifically associated with prespliceosome and spliceosome complexes
8. The splicing factor BBP interacts specifically with the pre-mRNA branchpoint sequence UACUAAC
9. Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels
10. Heat-shock but not other stress inducers leads to the disruption of a subset of snRNPs and inhibition of in vitro splicing in HeLa-cells
11. A Sm-like protein complex that participates in mRNA degradation
12. Reassembly and protection of small nuclear ribonucleoprotein particles by heat shock proteins in yeast cells
13. Splicing of precursors to mRNAs by the spliceosomes
14. The yeast U2A′/U2B″ complex is required for pre-spliceosome formation
15. Partial purification of the yeast U2 snRNP reveals a novel yeast pre-mRNA splicing factor required for pre-spliceosome assembly
16. Rna11 protein is associated with the yeast spliceosome and is localized in the periphery of the cell-nucleus
17. Snt309p, a component of the Prp19p-associated complex that interacts with Prp19p and associates with the spliceosome simultaneously with or immediately after dissociation of U4 in the same manner as Prp19p
18. Spliceosome assembly in yeast
19. Electron-microscopic identification of the yeast spliceosome
20. Characterization of a protein complex containing spliceosomal proteins SAPS 49, 130, 145, and 155
21. Functional association of U2 snRNP with the ATP-independent spliceosomal complex E
22. Variable flow liquid chromatography tandem mass spectrometry and the comprehensive analysis of complex protein digest mixtures
23. Rapid protein identification using a microscale electrospray LC/MS system on an ion trap mass spectrometer
24. The identification and characterization of a novel splicing protein, Isy1p, of Saccharomyces cerevisiae
25. Yeast U1 snRNP-pre-mRNA complex formation without U1 snRNA-pre-mRNA base pairing
26. An approach to correlate tandem mass-spectral data of peptides with amino-acid sequences in a protein database
27. Luc7p, a novel yeast U1 snRNP protein with role in 5′ splice site recognition
28. Synthetic lethal mutations suggest interactions between U5 small nuclear RNA and four proteins required for the second step of splicing
29. A comprehensive biochemical and genetic analysis of the yeast U1 snRNP reveals five novel proteins
30. Identification by mass spectrometry and functional analysis of novel proteins of the yeast [U4/U6•U5] tri-snRNP
31. A novel yeast U2 snRNP protein, Snu17p, is required for the first catalytic step of splicing and for progression of spliceosome assembly
32. Messenger RNA splicing in yeast: Clues to why the spliceosome is a ribonucleoprotein
33. Improvement of an "in-gel" digestion procedure for the micropreparation of internal protein fragments for amino acid sequencing
34. A U5 small nuclear ribonucleoprotein particle protein involved only in the 2nd step of premessenger RNA splicing in Saccharomyces cerevisiae
35. Conservation of structure and subunit interactions in yeast homologues of splicing factor 3b (SF3b) subunits
36. Rat1p and Xrn1p are functionally interchangeable exoribonucleases that are restricted to and required in the nucleus and cytoplasm, respectively
37. Characterization of U4 and U6 interactions with the 5′ splice site using a S. cerevisiae in vitro trans-splicing system
38. Association of U2, U4, U5, and U6 small nuclear ribonucleoproteins in a spliceosome-type complex in absence of precursor RNA
39. One-step PCR mediated strategy for the construction of conditionally expressed and epitope tagged yeast proteins
40. A yeast cap binding protein complex (yCBC) acts at an early step in pre-mRNA splicing
41. Yeast mRNA splicing in vitro
42. A novel genetic screen for snRNP assembly factors in yeast identifies a conserved protein, Sad1p, also required for pre- mRNA splicing
43. Functional recognition of the 5′ splice site by U4/U6.U5 tri-snRNP defines a novel ATP-dependent step in early spliceosome assembly
44. Myb-related fission yeast cdc5p is a component of a 40S snRNP-containing complex and is essential for pre-mRNA splicing
45. Splicing of precursors to mRNA by the spliceosome
46. Method for screening peptide fragment ion mass spectra prior to database searching
47. Recognition of the TACTAAC box during mRNA splicing in yeast involves base pairing to the U2-like snRNA
48. RNA unwinding in U4/U6 snRNPs requires ATP hydrolysis and the DEIH-box splicing factor Brr2
49. A spliceosomal recycling factor that reanneals U4 and U6 small nuclear ribonucleoprotein particles
50. An early hierarchic role of U1 small nuclear ribonucleoprotein particle in spliceosome assembly
51. Sm and Sm-like proteins assemble in two related complexes of deep evolutionary origin
52. Identification of functional U1 snRNA-pre-mRNA complexes committed to spliceosome assembly and splicing
53. Genetic studies of the PRP17 gene of Saccharomyces cerevisiae: A domain essential for function maps to a nonconserved region of the protein
54. Mechanical devices of the spliceosome: Motors, clocks, springs, and things
55. An RNA switch at the 5′ splice site requires ATP and the DEAD box protein Prp28p
56. Analysis of low-abundance RNPs from yeast by affinity chromatography and mass spectrometry microsequencing
57. Purification of the yeast U4/U6•U5 small nuclear ribonucleoprotein particle and identification of its proteins
58. Biochemical and genetic analyses of the U5, U6, and U4/U6•U5 small nuclear ribonucleoproteins from Saccharomyces cerevisiae
59. Functional association of essential splicing factors with Prp19 in a protein complex
60. The human U5 snRNP-specific 100-kD protein is an RS domain- containing, putative RNA helicase with significant homology to the yeast splicing factor Prp28p
61. Yeast Sm-like proteins function in mRNA decapping and decay
62. Cef1p is a component of the Prp19p-associated complex and essential for pre-mRNA splicing
63. A comprehensive analysis of protein-protein interactions in Saccharomyces cerevisiae
64. Spliceosomal UsnRNP biogenesis, structure and function
65. Synthetic lethality of yeast slt mutations with U2 small nuclear RNA mutations suggests functional interactions between U2 and U5 snRNPs that are important for both steps of pre-mRNA splicing
66. U4 small nuclear RNA dissociates from a yeast spliceosome and does not participate in the subsequent splicing reaction
67. Identification of eight proteins that cross-link to pre-mRNA in the yeast commitment complex
68. Subnuclear particles containing a small nuclear RNA and heterogeneous RNA