The DExH protein NPH-II is a processive and directional motor for unwinding RNA

Nature

Volumn 403, Issue 6768, 2000, Pages 447-451

  Jankowsky, Eckhard ^a   Gross, Christian H ^b   Shuman, Stewart ^b   Pyle, Anna Marie ^a,c  

^a Howard Hughes Medical Institute   (United States)

^b MEMORIAL SLOAN KETTERING CANCER CENTER   (United States)

^c HOWARD HUGHES MEDICAL INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DEOXYCYTIDINE KINASE; DEXH PROTEIN; NPH II PROTEIN; RNA; RNA HELICASE; UNCLASSIFIED DRUG;

ARTICLE; ENZYME ACTIVITY; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FAMILY; RNA DEGRADATION; RNA METABOLISM; RNA PROCESSING; VIRUS REPLICATION;

KINETICS; MOLECULAR MOTOR PROTEINS; RNA; RNA HELICASES; VACCINIA VIRUS;

EID: 0034719392     PISSN: 00280836     EISSN: None     Source Type: Journal    
DOI: 10.1038/35000239     Document Type: Article

References (22)

1. de la Cruz, J., Kressler, D. & Under, P. Unwinding RNA in Saccharomyces cereviseae: DEAD-box proteins and related families. Frends Biochem. Sci. 24, 192-198 (1999).
2. Kadare, G. & Haenni A. L. Virus encoded RNA heficaus. J. Virol. 71, 2583-2590 (1997).
3. Wagner, J. D. O., Jankowsky, E., Company, M., Pyle, A. M. & Abelson, J. N. The DEAH-box protein Prp22 is an ATPase that mediates ATP dependent mRNA release from the spliceosome and unwinds RNA duplexes. EMBO J. 17, 2926-2937 (1998).
4. Staley, J. P. & Guthrie, C. Mechanical devices of the spliceosome: motors clocks springs and things. Cell 90, 1041-1050 (1998).
5. Shuman, S. Vaccinia Virus RNA helicase: an essential enzyme related to the DE-H family of RNA dependent NTPases. Proc. Natl Acad. Sci. USA 89, 10935-10939 (1992).
6. Shuman, S. Vaccinia Virus RNA helicase. J. Biol. Chem. 268, 11798-11802 (1993).
7. Gross, C. H. & Shuman, S. Vaccinia virus RNA helicase: nucleic acid specificity in duplex unwinding. J. Virol. 70, 2615-2619 (1996).
8. Ali, J. A. & Lohman, T. M. Kinetic measurement of the step size of DNA unwinding by Escherischia coli UvrD helicase. Science 275, 377-380 (1997).
9. Shimamoto, N. One-dimensional diffusion of proteins along DNA. J. Biol. Chem. 274, 15239-15296 (1999).
10. Kelemen, B. R. & Raines, R. T. Extending the limits to enzymatic catalysis: diffusion of ribonuclease A in one dimension. Biochemistry 27, 5302-5307 (1999).
11. Jeltsch, A. & Pingoud, A. Kinetic characterization of linear diffusion of the restriction endonuclease EcoRV on DNA. Biochemistry 24, 2160-2169 (1998).
12. Santoro, S. W. & Joyce, G. F. A general purpose RNA-cleaving DNA enzyme. Proc. Natl Acad. Sci. USA 94, 4262-4266 (1997).
13. Lohman, T. M. & Bjoernson, K. P. Mechanisms of helicase catalyzed DNA unwinding. Annu. Rev. Biochem. 65, 169-214 (1996).
14. Schnapp, B. Molecular Motors. Two heads are better than one. Nature 373, 655-666 (1995).
15. Gross, C. H. & Shuman, S. Mutational analysis of vaccinia virus nucleoside triphosphate hydrolyase II, a DExH box RNA helicase. J. Virol. 69, 4727-4736 (1995).
16. Jankowsky, E. & Schwenzer, B. Efficient improvement of hammerhead ribozyme mediated cleavage of long substrates by oligonucleotide facilitators. Biochemisty 37, 15313-15321 (1996).
17. Xiang, Q., Qin, P., Michels, W. J., Freeland, K. & Pyle, A. M. Sequence specificity of a group II intron ribozyme: multiple mechanisms for promoting unusually high discrimination against mismatched targets. Biochemistry 37, 3839-3849 (1998).
18. Wincott, F. et al. Synthesis, deprotection, analysis and purification of RNA and ribozymes. Nucleic Acids Res. 23, 2677-2684 (1995).
19. Pyle, A. M., Chu, V. T., Jankowsky, E. & Boudvillain, M. Using DNAzymes to cut, process and map RNA molecules for structural studies or modification. Methods Enzymol. 317, 140-146 (2000).
20. Moore, M. J. & Sharp, P. A. Site specific modification of pre- messenger RNA - the 2′-hydroxyl groups at the splice sites. Science 256, 992-997 (1992).
21. Zimmerie, C. & Eneden, C. Analysis of progress curves by simulations generated by numerical integration. Biochem. J. 258, 363-387 (1989).
22. Barshop, B. A. & Frieden, C. Analysis of numerical methods for computer simulation of kinetic processes: development of KINSIM - a flexible portable system. Anal. Biochem. 130, 134-145 (1983).