Detection by denaturing gradient gel electrophoresis of pncA mutations associated with pyrazinamide resistance in Mycobacterium tuberculosis isolates from the United States-Mexico border region

Antimicrobial Agents and Chemotherapy

Volumn 49, Issue 6, 2005, Pages 2210-2217

  McCammon, Mark T ^a   Gillette, John S ^a   Thomas, Derek P ^a   Ramaswamy, Srinivas V ^b,c   Rosas, Ishmael I ^a   Graviss, Edward A ^b   Vijg, Jan ^a   Quitugua, Teresa N ^a  

^a Mays Cancer Center at UT Health San Antonio   (United States)

^b BAYLOR COLLEGE OF MEDICINE   (United States)

^c BRIGHAM AND WOMEN S HOSPITAL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CYSTEINE; DNA FRAGMENT; GLYCINE; PYRAZINAMIDE;

ANTIBIOTIC RESISTANCE; ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIAL GENE; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATE; DENATURING GRADIENT GEL ELECTROPHORESIS; DNA DETERMINATION; DNA SEQUENCE; GENE MUTATION; GENE PROBE; MELTING POINT; MEXICO; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; PNCA GENE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; UNITED STATES;

AMIDOHYDROLASES; ANTITUBERCULAR AGENTS; BASE SEQUENCE; CODON; DRUG RESISTANCE, BACTERIAL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HUMANS; MEXICO; MICROBIAL SENSITIVITY TESTS; MOLECULAR SEQUENCE DATA; MUTATION; MYCOBACTERIUM TUBERCULOSIS; PYRAZINAMIDE; SEQUENCE ANALYSIS, DNA; UNITED STATES;

EID: 19544373468     PISSN: 00664804     EISSN: None     Source Type: Journal    
DOI: 10.1128/AAC.49.6.2210-2217.2005     Document Type: Article

References (49)

1. American Thoracic Society, CDC., and the Infectious Diseases Society of America. 2003. Treatment of tuberculosis. Morb. Mortal. Wkly. Rep. 52:1-77.
2. Bounpheng, M., S. McGrath, D. Macias, N. van Orsouw, Y. Sun, D. Rines, and J. Vijg. 2003. Rapid, inexpensive scanning for all possible BRCA1 and BRCA2 gene sequence variants in a single assay: implications for genetic testing. J. Med. Genet. 40:e33.
3. Brown, T. J., O. Tansel, and G. L. French. 2000. Simultaneous identification and typing of multidrug-resistant Mycobacterium tuberculosis isolates by analysis of pncA and rpoB. J. Med. Microbiol. 49:651-656.
4. Cheng, S. J., L. Thibert, T. Sanchez, L. Heifets, and Y. Zhang. 2000. pncA mutations as a major mechanism of pyrazinamide resistance in Mycobacterium tuberculosis: spread of a monoresistant strain in Quebec, Canada. Antimicrob. Agents Chemother. 44:528-532.
5. Dale, J. W., D. Brittain, A. A. Cataldi, D. Cousins, J. T. Crawford, J. Driscoll, H. Heersma, T. Lillebaek, T. N. Quitugua, N. Raslogi, D. van Soolingen, and V. Wright. 2001. Spacer oligonucleotide typing of Mycobacterium tuberculosis: recommendations for standardized nomenclature. Int. J. Tuberc. Lung Dis. 5:216-219.
6. Davies, A. P., O. J. Billington, T. D. McHugh, D. A. Mitchison, and S. H. Gillespie. 2000. Comparison of phenotypic and genotypic methods for pyrazinamide susceptibility testing with Mycobacterium tuberculosis. J. Clin. Microbiol. 38:3686-3688.
7. Dhana, R. K., N. J. van Orsouw, I. Sigalas, C. Eng., and J. Vijg. 1998. Critical factors in the performance and cost of two-dimensional gene scanning: RB1 as a model. BioTechniques 25:664-675.
8. Endoh, T., A. Yagihashi, N. Uehara, D. Kobayashi, N. Tsuji, M. Nakamura, S. Hayashi, N. Fujii, and N. Watanabe. 2002. Pyrazinamide resistance associated with pncA gene mutation in Mycobacterium tuberculosis in Japan. Epidemiol. Infect. 128:337-342.
9. Escalante, P., S. Ramaswamy, H. Sanabria, H. Soini, X. Pan, O. Valiente-Castillo, and J. M. Musser. 1998. Genotypic characterization of drug-resistant Mycobacterium tuberculosis isolates from Peru. Tuber. Lung Dis. 89:111-118.
10. Fischer, S. G., and L. S. Lerman. 1979. Analysis of point mutations by denaturing gradient gel electrophoresis. Proc. Natl. Acad. Sci. USA 80:1579-1583.
11. Garcia-Delgado, M., C. J. Gonzalez-Navarro, M. C. Napal, C. Baldonado, J. L. Vizmanos, and A. Gullon. 1998. Higher sensitivity of denaturing gradient gel electrophoresis than sequencing in the detection of mutations in DNA from tumor samples. BioTechniques 24:72-76.
12. Gotoh, O. 1983. Prediction of melting profiles and local helix stability for sequenced DNA. Adv. Biophys. 16:1-52.
13. Gurtler, V., H. D. Barrie, and B. C. Mayall. 2002. Denaturing gradient gel electrophoresis multilocus sequence typing of Staphylococcus aureus isolates. Electrophoresis 23:3310-3320.
14. Hannan, M. M., E. P. Desmond, G. P. Morlock, G. H. Mazurek, and J. T. Crawford. 2001. Pyrazinamide-monoresistant Mycobacterium tuberculosis in the United States. J. Clin. Microbiol. 39:647-650.
15. Hou, L., D. Osei-Hyiaman, Z. Zhang, B. Wang, A. Yang, and K. Kano. 2000. Molecular characterization of pncA gene mutations in Mycobacterium tuberculosis clinical isolates from China. Epidemiol. Infect. 124:227-232.
16. Huang, T-S., S. S-J. Lee, H-Z. Tu, W-K. Huang, Y-S. Chen, C-K. Huang, S-R. Wann, H-H. Lin, and Y-C. Liu. 2003. Correlation between pyrazinamide activity and pncA mutations in Mycobacterium tuberculosis isolates in Taiwan. Antimicrob. Agents Chemother. 47:3672-3673.
17. Lemaitre, N., W. Sougakoff, C. Truffot-Pernot, and V. Jarlier. 1999. Characterization of new mutations in pyrazinamide-resistant strains of Mycobacterium tuberculosis and identification of conserved regions important for the catalytic activity of the pyrazinamidase PncA. Antimicrob. Agents Chemother. 43:1761-1763.
18. Liu, Y. P., M. A. Behr, P. M. Small, and N. Kurn. 2000. Genotypic determination of Mycobacterium tuberculosis antibiotic resistance using a novel mutation detection method, the branch migration inhibition M. tuberculosis antibiotic resistance test. J. Clin. Microbiol. 38:3656-3662.
19. Marttila, H. J., M. Marjamaki, E. Vyshnevskaya, B. I. Vyshnevskiy, T. F. Otten, A. V. Vasilyef, and M. K. Viljanen. 1999. pncA mutations in pyrazinamide-resistant Mycobacterium tuberculosis isolates from northwestern Russia. Antimicrob. Agents Chemother. 43:1764-1766.
20. McCammon, M. T., J. S. Gillette, D. P. Thomas, S. V. Ramaswamy, E. A. Graviss, B. N. Kreiswirth, J. Vijg, and T. N. Quitugua. 2005. Detection of rpoB mutations associated with rifampin resistance in Mycobacterium tuberculosis using denaturing gradient gel electrophoresis. Antimicrob. Agents Chemother. 49:2200-2209.
21. McGrath, S. B., M. Bounpheng, L. Torres, M. Calavetta, C. B. Scott, Y. Suh, D. Rines, N. van Orsouw, and J. Vijg. 2001. High-speed, multicolor fluorescent two-dimensional gene scanning. Genomics 78:83-90.
22. Mestdagh, M., P. A. Fonteyne, L. Realini, R. Rossau, G. Jannes, W. Mijs, K. A. L. De Smet, F. Portaels, and E. Van den Eeckhout. 1999. Relationship between pyrazinamide resistance, loss of pyrazinamidase activity, and mutations in the pncA locus in multidrug-resistant clinical isolates of Mycobacterium tuberculosis. Antimicrob. Agents Chemother. 43:2317-2319.
23. Mitchison, D. A. 2004. The search for new sterilizing anti-tuberculosis drugs. Front. Biosci. 9:1059-1072.
24. Miyagi, C., N. Yamane, B. Yogesh, H. Ano, and T. Takashima. 2004. Genetic and phenotypic characterization of pyrazinamide-resistant Mycobacterium tuberculosis complex isolates in Japan. Diagn. Microbiol. Infect. Dis. 48:111-116.
25. Mohamed, A. M., D. R. Bastola, G. P. Morlock, R. C. Cooksey, and S. H. Hinrichs. 2004. Temperature-mediated heteroduplex analysis for detection of pncA mutations associated with pyrazinamide resistance and differentiation between Mycobacterium tuberculosis and Mycobacterium bovis by denaturing high-performance liquid chromatography. J. Clin. Microbiol. 42:1016-1023.
26. Morlock, G. P., J. T. Crawford, W. R. Butler, S. E. Brim, D. Sikes, G. H. Mazurek, C. L. Woodley, and R. C. Cooksey. 2000. Phenotypic characterization of pncA mutants of Mycobacterium tuberculosis. Antimicrob. Agents Chemother. 44:2291-2295.
27. Park, S. K., J. Y. Lee, C. L. Chang, M. K. Lee, H. C. Son, C. M. Kim, H. J. Jang, H. K. Park, and S. H. Jeong. 2001. pncA mutations in clinical Mycobacterium tuberculosis isolates from Korea. BMC Infect. Dis. 1:4.
28. Portugal, I., L. Barreiro, J. Moniz-Pereira, and L. Brum. 2004. pncA mutations in pyrazinamide-resistant Mycobacterium tuberculosis isolates in Portugal. Antimicrob. Agents Chemother. 48:2736-2738.
29. Post, F. A., P. A. Willcox, B. Mathema, L. M. Steyn, K. Shean, S. V. Ramaswamy, E. A. Graviss, E. Shashkina, B. N. Kreiswirth, and G. Kaplan. 2004. Genetic polymorphism in Mycobacterium tuberculosis isolates from patients with chronic multidrug-resistant tuberculosis. J. Infect. Dis. 190:99-106.
30. Quitugua, T. N., B. J. Seaworth, S. E. Weis, J. P. Taylor, J. S. Gillette, I. I. Rosas, K. C. Jost, Jr., D. M. Magee, and R. A. Cox. 2002. Transmission of drug-resistant tuberculosis in Texas and Mexico. J. Clin. Microbiol. 40:2716-2724.
31. Ramaswamy, S., and J. M. Musser. 1998. Molecular genetic basis of antimicrobial agent resistance in Mycobacterium tuberculosis: 1998 update. Tuber. Lung Dis. 79:3-29.
32. Salfinger, M., L. B. Reller, B. Demchuk, and Z. T. Johnson. 1989. Rapid radiometric method for pyrazinamide susceptibility testing of Mycobacterium tuberculosis. Res. Microbiol. 140:301-309.
33. Scarpellini, P., S. Braglia, P. Carrera, M. Cedri, P. Cichero, A. Colombo, R. Crucianelli, A. Gori, M. Ferrari, and A. Lazzarin. 1999. Detection of rifampin resistance in Mycobacterium tuberculosis by double gradient-denaturing gradient gel electrophoresis. Antimicrob. Agents Chemother. 43:2550-2554.
34. Scorpio, A., and Y. Zhang. 1996. Mutations in pncA, a gene encoding pyrazinamidase/nicotinamidase, cause resistance to the antituberculous drug pyrazinamide in tubercle bacillus. Nat. Med. 2:662-667.
35. Scorpio, A., P. Lindholm-Levy, L. Heifets, R. Gilman, S. Siddiqi, M. Cynamon, and Y. Zhang. 1997. Characterization of pncA mutations in pyrazinamide-resistant Mycobacterium tuberculosis. Antimicrob. Agents Chemother. 41:540-543.
36. Sreevatsan, S., X. Pan, Y. Zhang, B. Kreiswirth, and J. M. Musser. 1997. Mutations associated with pyrazinamide resistance in pncA of Mycobacterium tuberculosis complex organisms. Antimicrob. Agents Chemother. 41: 636-640.
37. Steger, G. 1994. Thermal denaturation of double-stranded nucleic acids: prediction of temperatures critical for gradient gel electrophoresis and polymerase chain reaction. Nucleic Acids Res. 22:2760-2768.
38. Suzuki, Y., A. Suziki, A. Tamaru, C. Katsukawa, and H. Oda. 2002. Rapid detection of pyrazinamide-resistant Mycobacterium tuberculosis by a PCR-based in vitro system. J. Clin. Microbiol. 40:501-507.
39. Tracevska, T., I. Jansone, V. Baumanis, A. Nodieva, O. Marga, and G. Skenders. 2004. Spectrum of pncA mutations in multidrug-resistant Mycobacterium tuberculosis isolates obtained in Latvia. Antimicrob. Agents Chemother. 48:3209-3210.
40. Trulzsch, B., K. Krohn, P. Wonerow, and R. Paschke. 1999. DGGE is more sensitive for the detection of somatic point mutations than direct sequencing. BioTechniques. 27:266-268.
41. Urwin, R., and M. C. J. Maiden. 2003. Multi-locus sequence typing: a tool for global epidemiology. Trends Microbiol. 11:479-487.
42. van Embden, J. D., M. D. Cave, J. T. Crawford, J. W. Dale, K. D. Eisenach, B. Gicquel, P. Hermans, C. Martin, R. McAdam, and T. M. Shinnick. 1993. Strain identification of Mycobacterium tuberculosis by DNA fingerprinting: recommendations for a standardized methodology. J. Clin. Microbiol. 31: 406-409.
43. van Orsouw, N. J., R. K. Dhanda, R. D. Rines, W. M. Smith, I. Sigalas, C. Eng, and J. Vijg. 1998. Rapid design of denaturing gradient-based two-dimensional electrophoretic gene mutational scanning tests. Nucleic Acids Res. 26:2398-2406.
44. Victor, T. C., P. D. van Helden, and R. Warren. 2002. Prediction of drug resistance in M. tuberculosis: molecular mechanisms, tools and applications. IUBMB Life 53:231-237.
45. Vijg, J., and N. J. van Orsouw. 1999. Two-dimensional gene scanning: exploring human genetic variability. Electrophoresis 20:1239-1249.
46. Wade, M. M., D. Volokhov, M. Peredelchuk, V. Chizhikov, and Y. Zhang. 2004. Accurate mapping of mutations of pyrazinamide-resistant Mycobacterium tuberculosis strains with a scanning-frame oligonucleotide microarray. Diagn. Microbiol. Infect. Dis. 49:89-97.
47. Zhang, Y., and A. Telenti. 2000. Genetics of drug resistance in Mycobacterium tuberculosis, p. 235-254. In F. F. Hatfull and W. R. Jacobs, Jr. (ed.), Molecular genetics of mycobacteria. ASM Press, Washington, D.C.
48. Zhang, Y., and D. Mitchison. 2003. The curious characteristics of pyrazinamide: a review. Int. J. Tuberc. Lung Dis. 7:6-21.
49. Zhang, Y., M. M. Wade, A. Scorpio, H. Zhang, and Z. Sun. 2003. Mode of action of pyrazinamide: disruption of Mycobacterium tuberculosis membrane transport and energetics by pyrazinoic acid. J. Antimicrob. Chemother. 52: 790-795.