Higher sensitivity of denaturing gradient gel electrophoresis than sequencing in the detection of mutations in DNA from tumor samples

BioTechniques

Volumn 24, Issue 1, 1998, Pages 72-76

  Garcia Delgado M ^a   Gonzalez Navarro C J ^a   Napal, M C ^a   Baldonado, C ^a   Vizmanos, J L ^a   Gullon A ^a  

^a UNIVERSITY OF NAVARRA   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

ANDROGEN RECEPTOR; DNA; PROTEIN P53;

ALLELE; ARTICLE; DENATURING GRADIENT GEL ELECTROPHORESIS; EXON; HUMAN; ONCOGENE; POINT MUTATION; POLYMERASE CHAIN REACTION; SINGLE STRAND CONFORMATION POLYMORPHISM; TUMOR; TUMOR SUPPRESSOR GENE;

EID: 2642590009     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/98241bm15     Document Type: Article

References (12)

1. Andersen, T.I. and A.L. Børresen. 1995. Alterations of the TP53 gene as a potential prognostic marker in breast carcinomas. Diagn. Mol. Pathol. 4:203-211.
2. Andersen, T.I., R. Holm, J.M. Nesland, K.R. Heimdal, L. Ottestad and A.L. Børresen. 1993. Prognostic significance of TP53 alterations in breast carcinoma. Br. J. Cancer 68:540-548.
3. Beck, J.S., A.E. Kwitek, P.H. Cogen, A.K. Metzger, G.M. Duyk and W.C. Sheffield. 1993. A denaturing gradient gel electrophoresis assay for sensitive detection of p53 mutations. Hum. Genet. 91:25-30.
4. Børresen, A.L., T.I. Andersen, J. Garber, N. Barbier-Piraux, S. Thorlacius, J. Eyfjörd, L. Ottestad, B. Smith-Sørensen et al. 1992. Screening for germ line TP53 mutations in breast cancer patients. Cancer Res. 52:3234-3236.
5. Børresen, A.L., E. Hovig, B. Smith-Sørensen, D. Malkin, S. Lystad, T.I. Andersen, J.M. Nesland, K.J. Isselbacher and S.H. Friend. 1991. Constant denaturant gel electrophoresis as a rapid screening technique for p53 mutations. Proc. Natl. Acad. Sci. USA 88:8405-8409.
6. Bosari, S. and G. Viale. 1995. The clinical significance of p53 aberrations in human tumors. Virchows Arch. 427:229-241.
7. Carbone, D., Y. Chiba and T. Mitsudomi. 1991. Polymorphism at codon 213 within the p53 gene. Oncogene 6:1691-1692.
8. Hamelin, R., N. Jego, P. Laurent-Puig, M. Vidaud and G. Thomas. 1993. Efficient screening of p53 mutations by denaturing gradient gel electrophoresis in colorectal tumor. Oncogene 8:2213-2220.
9. Loda, M.D. 1994. Polymerase chain reaction-based methods for the detection of mutations in oncogenes and tumor suppressor genes. Hum. Pathol. 25:564-571.
10. Lynch, H.T. 1981. Genetics and Breast Cancer. Van Nostrand Reinhold, New York.
11. Moyret, C., C. Theillet, P. Laurent-Puig, J.P. Molés, G. Thomas and R. Hamelin. 1994. Relative efficiency of denaturing gradient gel electrophoresis and single strand conformation polymorphism in the detection of mutations in exons 5 to 8 of the p53 gene. Oncogene 9:1739-1743.
12. Newmark, J.R., D.O. Hardy, D.C. Tonb, B.S. Carter, J.I. Epstein, W.B. Isaacs, T.R. Brown and E.R. Barrack. 1992. Androgen receptor gene mutations in human prostate cancer. Proc. Natl. Acad. Sci. USA 89:6319-6323.