Characterization of new mutations in pyrazinamide-resistant strains of Mycobacterium tuberculosis and identification of conserved regions important for the catalytic activity of the pyrazinamidase PncA

Antimicrobial Agents and Chemotherapy

Volumn 43, Issue 7, 1999, Pages 1761-1763

  Lemaitre, Nadine ^a,b   Sougakoff, Wladimir ^a   Truffot Pernot, Chantal ^a   Jarlier, Vincent ^a  

^a SORBONNE UNIVERSITÉ   (France)

^b PITIÉ SALPÊTRIÈRE HOSPITAL   (France)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL ENZYME; PYRAZINAMIDASE; PYRAZINAMIDE; UNCLASSIFIED DRUG;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL GENE; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; GENE INSERTION; GENE MUTATION; GENETIC RESISTANCE; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; SPECIES DIFFERENCE;

EID: 0032799334     PISSN: 00664804     EISSN: None     Source Type: Journal    
DOI: 10.1128/aac.43.7.1761     Document Type: Article

References (11)

1. Escalante, P., S. Ramaswamy, H. Sanabria, H. Soini, X. Pan, O. Valiente-Castillo, and J. M. Musser. 1998. Genotypic characterization of drug-resistant Mycobacterium tuberculosis isolates from Peru. Tuber. Lung Dis. 79:111-118.
2. Fang, K., and K. J. Forbes. 1997. A Mycobacterium tuberculosis IS6110 preferential locus (ipl) for insertion into the genome. J. Clin. Microbiol. 35:479-481.
3. Fomukong, N., M. Beggs, H. El Hajj, G. Templeton, K. Eisenach, and M. D. Cave. 1998. Differences in the prevalence of IS6110 insertion sites in Mycobacterium tuberculosis strains: low and high copy number of IS6110. Tuber. Lung Dis. 78:109-116.
4. Hermans, P. W. M., D. Van Soolingen, E. M. Bik, P. E. W. De Haas, J. W. Dale, and J. D. A. Van Embden. 1991. Insertion element IS987 from Mycobacterium bovis BCG is located in a hot-spot integration region for insertion elements in Mycobacterium tuberculosis complex strains. Infect. Immun. 59: 2695-2705.
5. Hirano, K., M. Takahashi, Y. Kasumi, Y. Fukasawa, and C. Abe. 1998. Mutation in pncA is a major mechanism of pyrazinamide resistance in Mycobacterium tuberculosis. Tuber. Lung Dis. 78:117-122.
6. Ramaswamy, S., and J. M. Musser. 1998. Molecular genetic basis of antimicrobial agent resistance in Mycobacterium tuberculosis: 1998 update. Tuber. Lung Dis. 79:3-29.
7. Romao, M. J., D. Turk, F.-X. Gomis-Ruth, R. Huber, G. Schumacher, H. Möllering, and L. Russmann. 1992. Crystal structure analysis, refinement and enzymatic reaction mechanism of N-carbamoylsarcosine amidohydrolase from Arthrobacter sp. at 2.0 Å resolution. J. Mol. Biol. 226:1111-1130.
8. Scorpio, A., and Y. Zhang. 1996. Mutations in pncA, a gene encoding pyrazinamidase/nicotinamidase, cause resistance to the antituberculous drug pyrazinamide in tubercle bacillus. Nat. Med. 2:662-667.
9. Scorpio, A., P. Lindholm-Levy, L. Heifels, R. Gilman, S. Siddiqi, M. Cynamon, and Y. Zhang. 1997. Characterization of pncA mutations in pyrazinamide-resistant Mycobacterium tuberculosis. Antimicrob. Agents Chemother. 41:540-543.
10. Sreevatsan, S., X. Pan, Y. Zhang, B. N. Kreiswirth, and J. M. Musser. 1997. Mutations associated with pyrazinamide resistance in pncA of Mycobacterium tuberculosis complex organisms. Antimicrob. Agents Chemother. 41: 636-640.
11. Wayne, L. G. 1974. Simple pyrazinamidase and urease tests for routine identification of mycobacteria. Am. Rev. Respir. Dis. 199:147-151.