DGGE is more sensitive for the detection of somatic point mutations than direct sequencing

BioTechniques

Volumn 27, Issue 2, 1999, Pages 266-268

  Trülzsch, Barbara ^a   Krohn, Knut ^a   Wonerow, Peter ^a   Paschke, Ralf ^a  

^a UNIVERSITY OF LEIPZIG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; THYROTROPIN RECEPTOR;

ARTICLE; CONTAMINATION; DENATURING GRADIENT GEL ELECTROPHORESIS; DNA SEQUENCE; HUMAN; INTERMETHOD COMPARISON; POINT MUTATION; POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS; THYROID NODULE;

EID: 0344562885     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/99272bm10     Document Type: Article

References (11)

1. Cremonesi, L., S. Firpo, M. Ferrari, P.G. Righetti and C. Gelfi. 1997. Double-gradient DGGE for optimized detection of DNA point mutations. BioTechniques 22:326-330.
2. sα protein gene in 31 toxic thyroid nodules. J. Clin. Endocrinol. Metab. 82:3885-3891.
3. Führer, D., C. Kubisch, U. Scheibler, P. Lamesch, K. Krohn and R. Paschke. 1998. The extracellular thyrotropin receptor domain is not a major candidate for mutations in toxic thyroid nodules. Thyroid 11:997-1001.
4. Holzapfel, H.-P., P. Wonerow, W. von Petrykowski, M. Henschen, W.A. Scherbaum and R. Paschke. 1997. Sporadic congenital hyperthyroidism due to a spontaneous germline mutation in the thyrotropin receptor gene. J. Clin. Endocrinol. Metab. 82:3879-3884.
5. Maynard, J.H. and M. Upadhyaya. 1998. High-throughput screening for the detection of unknown mutations: improved productivity using heteroduplex analysis. BioTechniques 25:648-651.
6. sα genes as a cause of toxic thyroid adenomas. J. Clin. Endocrinol. Metab. 82:2695-2701.
7. Paschke, R. and M. Ludgate. 1997. The thyrotropin receptor in thyroid diseases. N. Engl. J. Med. 337:1675-1681.
8. PE Biosystems. PRISM™ - Tips 4. Foster, City, CA.
9. Prosser, J. 1993. Detecting single-base mutations. Trends Biotechnol. 11:238-246.
10. Schwaab, R., J. Oldenburg, M.R. Lalloz, U. Schwaab, S.I. Pemberton, P. Hanfland, H.H. Brackmann, E.G. Tuddenham and K. Michaelides. 1997. Factor VIII gene mutations found by a comparative study of SSCP, DGGE, and GMC and their analysis on a molecular model of factor VIII protein. Hum. Genet. 101:323-332.
11. Tonacchera, M., L. Chiovato, A. Pinchera, P. Agretti, E. Fiore, F. Cetani, R. Rocchi, P. Viaccava, P. Miccoli and P. Vitti. 1998. Hyperfunctioning thyroid nodules in toxic multinodular goiter share activating thyrotropin receptor mutations with solitary toxic adenoma. J. Clin. Endocrinol. Metab. 83:492-498.