Mutations in pncA, a gene encoding pyrazinamidase/nicotinamidase, cause resistance to the antituberculous drug pyrazinamide in tubercle bacillus

Nature Medicine

Volumn 2, Issue 6, 1996, Pages 662-667

  Scorpio, Angelo ^a   Zhang, Ying ^a  

^a JOHNS HOPKINS BLOOMBERG SCHOOL OF PUBLIC HEALTH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMIDASE; PYRAZINAMIDE;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL GENE; DRUG MECHANISM; GENE MUTATION; MOLECULAR CLONING; MULTIDRUG RESISTANCE; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; PRIORITY JOURNAL; TUBERCULOSIS;

AMIDOHYDROLASES; AMINO ACID SEQUENCE; BASE SEQUENCE; CLONING, MOLECULAR; DRUG RESISTANCE, MICROBIAL; MOLECULAR SEQUENCE DATA; MUTATION; MYCOBACTERIUM BOVIS; MYCOBACTERIUM TUBERCULOSIS; NICOTINAMIDASE; TRANSFORMATION, GENETIC;

BACTERIA (MICROORGANISMS); MYCOBACTERIUM; MYCOBACTERIUM BOVIS; MYCOBACTERIUM TUBERCULOSIS;

EID: 0029954860     PISSN: 10788956     EISSN: None     Source Type: Journal    
DOI: 10.1038/nm0696-662     Document Type: Article

References (18)

1. Centers for Disease Control. Nosocomial transmission of multidrug-resistant tuberculosis among HIV-infected persons -Florida and New York, 1988-1991. Mortal. Morbid. Weekly Rep. 40, 585-591 (1991).
2. Block, A.B. et al. Nationwide survey of drug-resistant tuberculosis in the United States. JAMA 271, 665-671 (1994).
3. Bloom, B.R. & Murray, C.J.L. Tuberculosis: Commentary on a reemergent killer. Science 257, 1055-1064 (1992).
4. Zhang, Y., Heym, B., Allen, B., Young, D. & Cole, S. The catalase-peroxidase gene and isoniazid resistance of Mycobacterium tuberculosis. Nature 358, 591-593 (1992).
5. Banerjee, A. et al. inhA, a gene encoding a target for isoniazid and ethionamide in Mycobacterium tuberculosis. Science 263, 227-230 (1994).
6. Telenti, A. et al. Detection of rifampicin-resistant mutations in Mycobacterium tuberculosis. Lancet 341, 647-650 (1993).
7. Finken, M., Kirschner, P., Meier, A., Wrede, A. & Bottger, E.C. Molecular basis of streptomycin resistance in Mycobacterium tuberculosis: Alterations of the ribosomal protein S12 gene and point mutations within a functional 16S ribosomal RNA pseudoknot. Mol. Microbiol. 9, 1239-1246 (1993).
8. Mitchison, D.A. The action of antituberculosis drugs in short course chemotherapy. Tubercle 66, 219-225 (1985).
9. McCune, R.M., Tompsett, R. & McDermott, W. The fate of Mycobacterium tuberculosis in mouse tissues as determined by the microbial enumeration technique. II. The conversion of tuberculous infection to the latent state by administration of pyrazinamide and a companion drug. J. Exp. Med. 104, 763-802 (1956).
10. Heifets, L. & Lindholm-Levy, P. Pyrazinamide sterilizing activity in vitro against semi-dormant Mycobacterium tuberculosis bacterial populations. Am. Rev. Respir. Dis. 145, 1223-1225 (1992).
11. Yeager, R.L., Munroe, W.G. & Dessau, F.I. Pyrazinamide (Aldinamide) in the treatment of pulmonary tuberculosis. Am. Rev. Tuberc. 65, 523-534 (1952).
12. Konno, K., Feldman, F.M. & McDermott, W. Pyrazinamide susceptibility and amidase activity of tubercle bacilli. Am. Rev. Respir. Dis. 95, 461-469 (1967).
13. McClatchy, J.K., Tsang, A.Y. & Cernich, M.S. Use of pyrazinamidase activity in Mycobacterium tuberculosis as a rapid method for determination of pyrazinamide susceptibility. Antimicrob. Agent. Chemother. 20, 556-557 (1981).
14. Trivedi, S.S. & Desai, S.G. Pyrazinamidase activity of Mycobacterium tuberculosis - a test of sensitivity to pyrazinamide. Tubercle 68, 221-224 (1987).
15. Konno, K., Nagayama, H. & Oka, S. Nicotinamidase in mycobacteria: A method for distinguishing bovine type tubercle bacilli from other mycobacteria. Nature 184, 1743-1744 (1959).
16. Collins, C.H., Grange, J.M. & Yates, M.D. Tuberculosis Bacteriology. 59-66 (Butterworths, London, 1985).
17. Foster, J.W. & Moat, A.G. Nicotinamide adenine dinucleotide biosynthesis and pyridine nucleotide cycle metabolism in microbial systems. Microbiol. Rev. 44, 83-105 (1980).
18. Bonicke, R. & Lisboa, B.P. Typendifferenzierung der Tuberkulosebakterien mit Hilfe des Nikotinamidasetests. Tuberkuloseartzt 13, 377 (1959).