Characterisation of G418-induced metabolic load in recombinant CHO and BHK cells: Effect on the activity and expression of central metabolic enzymes

Cytotechnology

Volumn 42, Issue 2, 2003, Pages 87-99

  Yallop, C A ^a,b   Norby P L ^a   Jensen, R ^a   Reinbach, H ^a   Svendsen, I ^a  

^a NOVO NORDISK A S   (Denmark)

^b CRUCELL HOLLAND BV   (Netherlands)

Author keywords

BHK; CHO; Enzymes; G418; Metabolic load; Metabolism; RT PCR

Indexed keywords

ANTIBIOTIC G 418; CELL ENZYME; CELL EXTRACT; CELL PROTEIN; GASTRIC INHIBITORY POLYPEPTIDE; GASTRIC INHIBITORY POLYPEPTIDE RECEPTOR; GLUCAGON RECEPTOR; GLUCOSE; GLUCOSE 6 PHOSPHATE DEHYDROGENASE; GLUTAMATE AMMONIA LIGASE; GLUTAMATE DEHYDROGENASE; GLUTAMINASE; GLUTAMINE; LACTATE DEHYDROGENASE; LACTIC ACID; MEMBRANE RECEPTOR; PYRUVATE CARBOXYLASE; RECOMBINANT RECEPTOR; UNCLASSIFIED DRUG;

AMINO ACID METABOLISM; ANALYTIC METHOD; ANIMAL CELL; ARTICLE; CALCULATION; CELL GROWTH; CELL METABOLISM; CELL STRAIN; CELL STRAIN BHK; CHEMICAL REACTION; CHO CELL; CITRIC ACID CYCLE; CONCENTRATION RESPONSE; CONTROLLED STUDY; CORRELATION ANALYSIS; CULTURE MEDIUM; ENZYME ACTIVITY; ENZYME ASSAY; GLUCOSE METABOLISM; GROWTH RATE; METABOLIC PARAMETERS; NONHUMAN; NUTRIENT DYNAMICS; PENTOSE PHOSPHATE CYCLE; PROTEIN DETERMINATION; PROTEIN EXPRESSION; PROTEIN PROCESSING; QUANTITATIVE ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

ANIMALIA;

EID: 1642460583     PISSN: 09209069     EISSN: None     Source Type: Journal    
DOI: 10.1023/B:CYTO.0000009821.82741.8c     Document Type: Article

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