Metabolic burden in recombinant CHO cells: effect of dhfr gene amplification and lacZ expression

Cytotechnology

Volumn 18, Issue 3, 1995, Pages 159-166

  Gu, Man Bock ^a   Todd, Paul ^b   Kompala, Dhinakar S ^a  

^a UNIVERSITY OF COLORADO   (United States)

^b UNIVERSITY OF DELAWARE   (United States)

Author keywords

CMV promoter; dhfr; foreign gene expression; gene amplification; growth rate reduction; metabolic burden; recombinant CHO cells; SV40 promoter

Indexed keywords

EID: 0030066488     PISSN: 09209069     EISSN: 15730778     Source Type: Journal    
DOI: 10.1007/BF00767763     Document Type: Article

References (20)

1. Selective multiplication of dihydrofolate reductase genes in methotrexate-resistant various of cultured murine cells
2. Amplification of the matallothionein-I gene in cadmium-resistant mouse cells
3. Mutation at autosomal loci of Chinese hamster ovary cells: Involvement of a high-frequency event silencing two linked alleles
4. Molecular cloning of DNA sequences complementary to mouse thymidylate synthase messenger RNA
5. Effect of amplification of dhfr and lacZ genes on growth and β-galactosidase expression in suspension cultures of recombinant CHO cells
6. Foreign gene expression (β-galactosidase) during the cell cycle phases in cerombinant CHO cells
7. Overexpression of the glucocorticoid receptor represses transcription from hormone responsive and non-responsive promoters
8. Relative promoter activity in human mammary epithelial cells assayed by transient expression
9. Coamplification and coexpression of human tissue-type plasminogen activator and murine dihydrofolate reductase sequences in Chinese hamster ovary cells
10. Synthesis, processing, and secretion of recombinant human factor VIII expressed in mammalian cells
11. Effect of cloned gene dosage on cell growth and Hepatitis B surface antigen synthesis and secretion in recombinant CHO cells
12. Amplification of P450c21 expression in cultured mammalian cells
13. Carbohydrate structure of erythropoietin expressed in Chinese hamster ovary cells by a human erythropoietin cDNA
14. Expression and characterization of the product of a human interferon cDNA gene in Chinese hamster ovary cells
15. Gene amplification and drug resistance in cultured murine cells
16. Relationship between sugar chain structure and biological activity of recombinant human erythropoietin produced in Chinese hamster ovary cells
17. Genetic Engineering in Mammalian Cells
18. Integration, amplification, and stability of plasmid suquences in CHO cell cultures
19. Mouse 3T6 cells that overproduce glutamine synthetase